Rapid detection of tetracycline residues at clinically relevant levels via a novel, inexpensive and analytically-sensitive magneto-immunoassay – A feasibility study
Expensive, time-consuming and labour-intensive solvent-extraction and liquid-chromatography methods are the industry's current gold standard for antibiotic residue quantification. A novel immunoassay methodology and system for the rapid detection of clinically relevant levels of tetracycline re...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Sensing and Bio-Sensing Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214180423000181 |
| _version_ | 1827859560555085824 |
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| author | Owen J. Harrison Bertrand Monnier Ed Regan Dave West Hugh Ballantine Dykes Jeffrey S. Davey Janice Kiely Richard Luxton |
| author_facet | Owen J. Harrison Bertrand Monnier Ed Regan Dave West Hugh Ballantine Dykes Jeffrey S. Davey Janice Kiely Richard Luxton |
| author_sort | Owen J. Harrison |
| collection | DOAJ |
| description | Expensive, time-consuming and labour-intensive solvent-extraction and liquid-chromatography methods are the industry's current gold standard for antibiotic residue quantification. A novel immunoassay methodology and system for the rapid detection of clinically relevant levels of tetracycline residues found in food-producing animal tissues is described. Anti-tetracycline antibody-coated paramagnetic particles were used for the specific capture of tetracycline in spiked buffer (with and without a 1% pork muscle tissue suspension) and quantified via an analytically-sensitive in-house magnetometer instrument. Detection of tetracycline between 0.1 μg/mL - 1 μg/mL was achieved, with a readout time (including sample treatment) presented in 20 min. The magneto-immunoassay described provides a rapid, low-cost, de-skilled and analytically-sensitive solution for tetracycline screening at the point-of-sampling, with potential applications for other prevalent antibiotic families used in the international farming and food industry. |
| first_indexed | 2024-03-12T13:10:12Z |
| format | Article |
| id | doaj.art-54c02ab38ea24639933721fa7688cc28 |
| institution | Directory Open Access Journal |
| issn | 2214-1804 |
| language | English |
| last_indexed | 2024-03-12T13:10:12Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensing and Bio-Sensing Research |
| spelling | doaj.art-54c02ab38ea24639933721fa7688cc282023-08-28T04:22:47ZengElsevierSensing and Bio-Sensing Research2214-18042023-08-0141100566Rapid detection of tetracycline residues at clinically relevant levels via a novel, inexpensive and analytically-sensitive magneto-immunoassay – A feasibility studyOwen J. Harrison0Bertrand Monnier1Ed Regan2Dave West3Hugh Ballantine Dykes4Jeffrey S. Davey5Janice Kiely6Richard Luxton7Institute of Bio-Sensing Technology, University of the West of England, Bristol BS16 1QY, UKCentre for Research in Biomedicine, University of the West of England, Bristol BS16 1QY, UKMIATech BioSolutions, Porton Science Park, Porton Down, Salisbury SP40BF, UKMIATech BioSolutions, Porton Science Park, Porton Down, Salisbury SP40BF, UKMIATech BioSolutions, Porton Science Park, Porton Down, Salisbury SP40BF, UKInstitute of Bio-Sensing Technology, University of the West of England, Bristol BS16 1QY, UKInstitute of Bio-Sensing Technology, University of the West of England, Bristol BS16 1QY, UKInstitute of Bio-Sensing Technology, University of the West of England, Bristol BS16 1QY, UK; Corresponding author.Expensive, time-consuming and labour-intensive solvent-extraction and liquid-chromatography methods are the industry's current gold standard for antibiotic residue quantification. A novel immunoassay methodology and system for the rapid detection of clinically relevant levels of tetracycline residues found in food-producing animal tissues is described. Anti-tetracycline antibody-coated paramagnetic particles were used for the specific capture of tetracycline in spiked buffer (with and without a 1% pork muscle tissue suspension) and quantified via an analytically-sensitive in-house magnetometer instrument. Detection of tetracycline between 0.1 μg/mL - 1 μg/mL was achieved, with a readout time (including sample treatment) presented in 20 min. The magneto-immunoassay described provides a rapid, low-cost, de-skilled and analytically-sensitive solution for tetracycline screening at the point-of-sampling, with potential applications for other prevalent antibiotic families used in the international farming and food industry.http://www.sciencedirect.com/science/article/pii/S2214180423000181Magneto-immunoassayMagnetometerBiosensorParamagnetic particlesTetracyclineAntibiotic detection |
| spellingShingle | Owen J. Harrison Bertrand Monnier Ed Regan Dave West Hugh Ballantine Dykes Jeffrey S. Davey Janice Kiely Richard Luxton Rapid detection of tetracycline residues at clinically relevant levels via a novel, inexpensive and analytically-sensitive magneto-immunoassay – A feasibility study Sensing and Bio-Sensing Research Magneto-immunoassay Magnetometer Biosensor Paramagnetic particles Tetracycline Antibiotic detection |
| title | Rapid detection of tetracycline residues at clinically relevant levels via a novel, inexpensive and analytically-sensitive magneto-immunoassay – A feasibility study |
| title_full | Rapid detection of tetracycline residues at clinically relevant levels via a novel, inexpensive and analytically-sensitive magneto-immunoassay – A feasibility study |
| title_fullStr | Rapid detection of tetracycline residues at clinically relevant levels via a novel, inexpensive and analytically-sensitive magneto-immunoassay – A feasibility study |
| title_full_unstemmed | Rapid detection of tetracycline residues at clinically relevant levels via a novel, inexpensive and analytically-sensitive magneto-immunoassay – A feasibility study |
| title_short | Rapid detection of tetracycline residues at clinically relevant levels via a novel, inexpensive and analytically-sensitive magneto-immunoassay – A feasibility study |
| title_sort | rapid detection of tetracycline residues at clinically relevant levels via a novel inexpensive and analytically sensitive magneto immunoassay a feasibility study |
| topic | Magneto-immunoassay Magnetometer Biosensor Paramagnetic particles Tetracycline Antibiotic detection |
| url | http://www.sciencedirect.com/science/article/pii/S2214180423000181 |
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