Characterization of the <i>BnA10.tfl1</i> Gene Controls Determinate Inflorescence Trait in <i>Brassica napus</i> L.
Determinate inflorescences have a significant effect on the genetic improvement of rapeseed, so understanding the molecular function underlying the inflorescence trait may be beneficial to oilseed breeding. A previous study found candidate gene <i>BnTFL1</i> (<i>Terminal Flower 1&l...
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MDPI AG
2019-11-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/9/11/722 |
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| author | Yongpeng Jia Kaixiang Li Haidong Liu Lingxiong Zan Dezhi Du |
| author_facet | Yongpeng Jia Kaixiang Li Haidong Liu Lingxiong Zan Dezhi Du |
| author_sort | Yongpeng Jia |
| collection | DOAJ |
| description | Determinate inflorescences have a significant effect on the genetic improvement of rapeseed, so understanding the molecular function underlying the inflorescence trait may be beneficial to oilseed breeding. A previous study found candidate gene <i>BnTFL1</i> (<i>Terminal Flower 1</i>) for control of the inflorescence trait on <i>Brassica napus</i> chromosome A10 (16,627−16,847 kb). However, little is known about the function of the <i>BnTFL1</i> gene in <i>B. napus</i>. In this study, we firstly studied the formation of the shoot apical meristem and gene expression in indeterminate and determinate inflorescences; the results showed that the inflorescence architecture and <i>BnA10.TFL1</i> expression showed significant differences in the shoot apex at the budding stage. Then, two alleles (named <i>BnA10.TFL1</i> gene from indeterminate and <i>BnA10.tfl1</i> gene from determinate) were cloned and sequence-analyzed; the results suggest that the open reading frame of the alleles comprises 537 bp, encodes 178 amino acids containing a conserved phosphatidylethanolamine-binding protein (PEBP) domain, and shares high similarity with <i>Arabidopsis</i> <i>thaliana</i> TFL1. To analyze the function of <i>BnA10.TFL1</i>, the <i>BnA10.TFL1</i> gene was introduced into the determinate <i>A. thaliana</i> <i>tfl1</i> mutant and <i>B. napus</i> 571 line by complementation experiment. The determinate traits were restored to indeterminate, and expression of <i>BnA10.TFL1</i> was increased in the indeterminate shoot apex. These results reveal that <i>BnA10.tfl1</i> is a gene controlling the determinate inflorescence trait. Moreover, the BnA10.TFL1 protein was localized to the nucleus, cytoplasm, and plasma membrane. Collectively, the results of this study help us to understand the molecular mechanism of determinate inflorescences and will provide a reliable research basis for the application of determinate inflorescences in <i>B. napus</i>. |
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| institution | Directory Open Access Journal |
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| spelling | doaj.art-54c2b2a4330e47f48a3a4b5e6724102f2022-12-21T23:35:52ZengMDPI AGAgronomy2073-43952019-11-0191172210.3390/agronomy9110722agronomy9110722Characterization of the <i>BnA10.tfl1</i> Gene Controls Determinate Inflorescence Trait in <i>Brassica napus</i> L.Yongpeng Jia0Kaixiang Li1Haidong Liu2Lingxiong Zan3Dezhi Du4State Key Laboratory of Plateau Ecology and Agriculture of Qinghai University, Academy of Agricultural and Forestry Sciences, Qinghai University, Xining 810016, Qinghai, ChinaState Key Laboratory of Plateau Ecology and Agriculture of Qinghai University, Academy of Agricultural and Forestry Sciences, Qinghai University, Xining 810016, Qinghai, ChinaState Key Laboratory of Plateau Ecology and Agriculture of Qinghai University, Academy of Agricultural and Forestry Sciences, Qinghai University, Xining 810016, Qinghai, ChinaState Key Laboratory of Plateau Ecology and Agriculture of Qinghai University, Academy of Agricultural and Forestry Sciences, Qinghai University, Xining 810016, Qinghai, ChinaState Key Laboratory of Plateau Ecology and Agriculture of Qinghai University, Academy of Agricultural and Forestry Sciences, Qinghai University, Xining 810016, Qinghai, ChinaDeterminate inflorescences have a significant effect on the genetic improvement of rapeseed, so understanding the molecular function underlying the inflorescence trait may be beneficial to oilseed breeding. A previous study found candidate gene <i>BnTFL1</i> (<i>Terminal Flower 1</i>) for control of the inflorescence trait on <i>Brassica napus</i> chromosome A10 (16,627−16,847 kb). However, little is known about the function of the <i>BnTFL1</i> gene in <i>B. napus</i>. In this study, we firstly studied the formation of the shoot apical meristem and gene expression in indeterminate and determinate inflorescences; the results showed that the inflorescence architecture and <i>BnA10.TFL1</i> expression showed significant differences in the shoot apex at the budding stage. Then, two alleles (named <i>BnA10.TFL1</i> gene from indeterminate and <i>BnA10.tfl1</i> gene from determinate) were cloned and sequence-analyzed; the results suggest that the open reading frame of the alleles comprises 537 bp, encodes 178 amino acids containing a conserved phosphatidylethanolamine-binding protein (PEBP) domain, and shares high similarity with <i>Arabidopsis</i> <i>thaliana</i> TFL1. To analyze the function of <i>BnA10.TFL1</i>, the <i>BnA10.TFL1</i> gene was introduced into the determinate <i>A. thaliana</i> <i>tfl1</i> mutant and <i>B. napus</i> 571 line by complementation experiment. The determinate traits were restored to indeterminate, and expression of <i>BnA10.TFL1</i> was increased in the indeterminate shoot apex. These results reveal that <i>BnA10.tfl1</i> is a gene controlling the determinate inflorescence trait. Moreover, the BnA10.TFL1 protein was localized to the nucleus, cytoplasm, and plasma membrane. Collectively, the results of this study help us to understand the molecular mechanism of determinate inflorescences and will provide a reliable research basis for the application of determinate inflorescences in <i>B. napus</i>.https://www.mdpi.com/2073-4395/9/11/722<i>brassica napus</i><i>terminal flower 1</i>indeterminatedeterminatetransformationquantitative real-time pcr |
| spellingShingle | Yongpeng Jia Kaixiang Li Haidong Liu Lingxiong Zan Dezhi Du Characterization of the <i>BnA10.tfl1</i> Gene Controls Determinate Inflorescence Trait in <i>Brassica napus</i> L. Agronomy <i>brassica napus</i> <i>terminal flower 1</i> indeterminate determinate transformation quantitative real-time pcr |
| title | Characterization of the <i>BnA10.tfl1</i> Gene Controls Determinate Inflorescence Trait in <i>Brassica napus</i> L. |
| title_full | Characterization of the <i>BnA10.tfl1</i> Gene Controls Determinate Inflorescence Trait in <i>Brassica napus</i> L. |
| title_fullStr | Characterization of the <i>BnA10.tfl1</i> Gene Controls Determinate Inflorescence Trait in <i>Brassica napus</i> L. |
| title_full_unstemmed | Characterization of the <i>BnA10.tfl1</i> Gene Controls Determinate Inflorescence Trait in <i>Brassica napus</i> L. |
| title_short | Characterization of the <i>BnA10.tfl1</i> Gene Controls Determinate Inflorescence Trait in <i>Brassica napus</i> L. |
| title_sort | characterization of the i bna10 tfl1 i gene controls determinate inflorescence trait in i brassica napus i l |
| topic | <i>brassica napus</i> <i>terminal flower 1</i> indeterminate determinate transformation quantitative real-time pcr |
| url | https://www.mdpi.com/2073-4395/9/11/722 |
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