Effect of Diesel Exhaust Particles on House Dust Mite–Induced Airway Eosinophilic Inflammation and Remodeling in Mice
Recent research has focused on the effects of ambient particulate pollution and much evidence has indicated that particulate pollution is associated with the onset of asthma and allergy; however, the effect of diesel exhaust particles (DEP) on the development of allergen-induced airway remodeling ha...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2010-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319310229 |
| _version_ | 1818922475653693440 |
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| author | Go Takahashi Hiroyuki Tanaka Keiko Wakahara Reishi Nasu Mikiko Hashimoto Kosuke Miyoshi Hirohisa Takano Hirotaka Yamashita Naoki Inagaki Hiroichi Nagai |
| author_facet | Go Takahashi Hiroyuki Tanaka Keiko Wakahara Reishi Nasu Mikiko Hashimoto Kosuke Miyoshi Hirohisa Takano Hirotaka Yamashita Naoki Inagaki Hiroichi Nagai |
| author_sort | Go Takahashi |
| collection | DOAJ |
| description | Recent research has focused on the effects of ambient particulate pollution and much evidence has indicated that particulate pollution is associated with the onset of asthma and allergy; however, the effect of diesel exhaust particles (DEP) on the development of allergen-induced airway remodeling has not been fully investigated in vivo. In the present study, we examined the effects of DEP on Dermatophagoides farinae allergens (Der f)–induced asthma-like phenotypes in mice. Mice were administered i.t. 8 times with Der f. DEP were injected i.t. with Der f 4 times throughout the experiment or twice at the sensitization period. In both cases, DEP aggravated Der f–induced increases in airway responsiveness to acetylcholine, the number of eosinophils and neutrophils in the bronchoalveolar lavage fluid (BALF), serum Der f–specific IgG1 levels, Th2 cytokines and transforming growth factor-β 1 levels in BALF, and amount of hydroxyproline in the right lungs. Furthermore, goblet cell hyperplasia and subepithelial fibrosis were also markedly aggravated. These findings indicate that DEP can potentiate airway remodeling induced by repeated allergen challenge as well as Th2-drived airway hyperresponsiveness, eosinophilic inflammation, and IgG1 production and that DEP can exhibit adjuvant activity for airway remodeling, probably due to the enhancement of allergen sensitization and/or of Th2 polarizing pathways. Keywords:: bronchial asthma, Dermatophagoides farinae, goblet cell, subepithelial fibrosis, transforming growth factor-β1 |
| first_indexed | 2024-12-20T01:54:08Z |
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| id | doaj.art-54c7be3c20c94408bb4550aac958666e |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-20T01:54:08Z |
| publishDate | 2010-01-01 |
| publisher | Elsevier |
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| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-54c7be3c20c94408bb4550aac958666e2022-12-21T19:57:32ZengElsevierJournal of Pharmacological Sciences1347-86132010-01-011122192202Effect of Diesel Exhaust Particles on House Dust Mite–Induced Airway Eosinophilic Inflammation and Remodeling in MiceGo Takahashi0Hiroyuki Tanaka1Keiko Wakahara2Reishi Nasu3Mikiko Hashimoto4Kosuke Miyoshi5Hirohisa Takano6Hirotaka Yamashita7Naoki Inagaki8Hiroichi Nagai9Laboratory of Pharmacology, Department of Bioactive Molecules, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, Japan; United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Gifu 501-1193, JapanLaboratory of Pharmacology, Department of Bioactive Molecules, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, Japan; United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Gifu 501-1193, Japan; Corresponding author. hirotnk@gifu-pu.ac.jpLaboratory of Pharmacology, Department of Bioactive Molecules, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, Japan; Division of Respiratory Medicine, Nagoya University Graduate School of Medicine, 65 Tsurumai, Syowa-ku, Nagoya, Aichi 466-8550, JapanLaboratory of Pharmacology, Department of Bioactive Molecules, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, JapanLaboratory of Pharmacology, Department of Bioactive Molecules, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, JapanLaboratory of Pharmacology, Department of Bioactive Molecules, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, JapanNational Institute for Environmental Studies, 16-2 Onokawa, Tsukuba, Ibaraki 305-8506, JapanLaboratory of Pharmacology, Department of Bioactive Molecules, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, Japan; United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Gifu 501-1193, JapanLaboratory of Pharmacology, Department of Bioactive Molecules, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, Japan; United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Gifu 501-1193, JapanLaboratory of Pharmacology, Department of Bioactive Molecules, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, JapanRecent research has focused on the effects of ambient particulate pollution and much evidence has indicated that particulate pollution is associated with the onset of asthma and allergy; however, the effect of diesel exhaust particles (DEP) on the development of allergen-induced airway remodeling has not been fully investigated in vivo. In the present study, we examined the effects of DEP on Dermatophagoides farinae allergens (Der f)–induced asthma-like phenotypes in mice. Mice were administered i.t. 8 times with Der f. DEP were injected i.t. with Der f 4 times throughout the experiment or twice at the sensitization period. In both cases, DEP aggravated Der f–induced increases in airway responsiveness to acetylcholine, the number of eosinophils and neutrophils in the bronchoalveolar lavage fluid (BALF), serum Der f–specific IgG1 levels, Th2 cytokines and transforming growth factor-β 1 levels in BALF, and amount of hydroxyproline in the right lungs. Furthermore, goblet cell hyperplasia and subepithelial fibrosis were also markedly aggravated. These findings indicate that DEP can potentiate airway remodeling induced by repeated allergen challenge as well as Th2-drived airway hyperresponsiveness, eosinophilic inflammation, and IgG1 production and that DEP can exhibit adjuvant activity for airway remodeling, probably due to the enhancement of allergen sensitization and/or of Th2 polarizing pathways. Keywords:: bronchial asthma, Dermatophagoides farinae, goblet cell, subepithelial fibrosis, transforming growth factor-β1http://www.sciencedirect.com/science/article/pii/S1347861319310229 |
| spellingShingle | Go Takahashi Hiroyuki Tanaka Keiko Wakahara Reishi Nasu Mikiko Hashimoto Kosuke Miyoshi Hirohisa Takano Hirotaka Yamashita Naoki Inagaki Hiroichi Nagai Effect of Diesel Exhaust Particles on House Dust Mite–Induced Airway Eosinophilic Inflammation and Remodeling in Mice Journal of Pharmacological Sciences |
| title | Effect of Diesel Exhaust Particles on House Dust Mite–Induced Airway Eosinophilic Inflammation and Remodeling in Mice |
| title_full | Effect of Diesel Exhaust Particles on House Dust Mite–Induced Airway Eosinophilic Inflammation and Remodeling in Mice |
| title_fullStr | Effect of Diesel Exhaust Particles on House Dust Mite–Induced Airway Eosinophilic Inflammation and Remodeling in Mice |
| title_full_unstemmed | Effect of Diesel Exhaust Particles on House Dust Mite–Induced Airway Eosinophilic Inflammation and Remodeling in Mice |
| title_short | Effect of Diesel Exhaust Particles on House Dust Mite–Induced Airway Eosinophilic Inflammation and Remodeling in Mice |
| title_sort | effect of diesel exhaust particles on house dust mite induced airway eosinophilic inflammation and remodeling in mice |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319310229 |
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