Association between Yogurt Consumption and Intestinal Microbiota in Healthy Young Adults Differs by Host Gender
Human intestinal microbiota are influenced by various factors viz. diet, environment, age, gender, geographical, and socioeconomic situation, etc. among which diet has the most profound impact. However, studies investigating this impact have mostly included subjects from diverse geographic/socioecon...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00847/full |
| _version_ | 1828412545037238272 |
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| author | Yoshio Suzuki Keiichi Ikeda Kazuhiko Sakuma Sachio Kawai Keisuke Sawaki Takashi Asahara Takashi Asahara Takuya Takahashi Takuya Takahashi Hirokazu Tsuji Hirokazu Tsuji Koji Nomoto Koji Nomoto Ravinder Nagpal Chongxin Wang Satoru Nagata Satoru Nagata Yuichiro Yamashiro |
| author_facet | Yoshio Suzuki Keiichi Ikeda Kazuhiko Sakuma Sachio Kawai Keisuke Sawaki Takashi Asahara Takashi Asahara Takuya Takahashi Takuya Takahashi Hirokazu Tsuji Hirokazu Tsuji Koji Nomoto Koji Nomoto Ravinder Nagpal Chongxin Wang Satoru Nagata Satoru Nagata Yuichiro Yamashiro |
| author_sort | Yoshio Suzuki |
| collection | DOAJ |
| description | Human intestinal microbiota are influenced by various factors viz. diet, environment, age, gender, geographical, and socioeconomic situation, etc. among which diet has the most profound impact. However, studies investigating this impact have mostly included subjects from diverse geographic/socioeconomic backgrounds and hence the precise effects of dietary factors on gut microbiota composition remain largely confounded. Herein, with an aim to evaluate the association between dietary habits, specifically yogurt consumption, and the gut microbiota in healthy young adults sharing similar age, lifestyle routine, geographical setting, etc., we conducted a cross-sectional study wherein 293 collegiate freshmen answered a questionnaire about their frequency of yogurt consumption over the last 2 months and provided stool specimens for microbiota analysis. Fecal microbiota were analyzed by highly sensitive reverse-transcription-quantitative-PCR assays targeting bacterial 16S rRNA molecules. Fecal organic acids were measured by HPLC. Overall, the gut microbiota were predominated (97.1 ± 8.6%) by Clostridium coccoides group, Clostridium leptum subgroup, Bacteroides fragilis group, Bifidobacterium and Atopobium cluster. Interestingly, after adjusting the data for yogurt consumption, females were found to have higher total bacterial (P = 0.013) and Bifidobacterium (P = 0.046) count and fecal pH (P = 0.007) and lower fecal concentration of total organic acids (P = 0.030), succinic acid (P = 0.007) and formic acid (P = 0.046) as compared to males. Altogether, yogurt consumption showed positive linear association with Lactobacillus and Lactobacillus gasseri subgroup in both male and female subjects; however, several gender-specific disparities were also detected in this yogurt-microbiota association. Yogurt consumption demonstrated a negative association with L. sakei subgroup, Enterobacteriaceae and Staphylococcus in males but shared a positive association with L. casei subgroup and succinic acid in female subjects. The study manifests the association between yogurt consumption and gut microbiota in a healthy homogeneous cohort and show how this association can differ by host gender. The findings should be helpful for prospective studies investigating the diet–microbiome interaction in human health and disease. |
| first_indexed | 2024-12-10T12:51:11Z |
| format | Article |
| id | doaj.art-54c8f3d0a75240e483194c76f6025ce2 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T12:51:11Z |
| publishDate | 2017-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-54c8f3d0a75240e483194c76f6025ce22022-12-22T01:48:15ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-05-01810.3389/fmicb.2017.00847262929Association between Yogurt Consumption and Intestinal Microbiota in Healthy Young Adults Differs by Host GenderYoshio Suzuki0Keiichi Ikeda1Kazuhiko Sakuma2Sachio Kawai3Keisuke Sawaki4Takashi Asahara5Takashi Asahara6Takuya Takahashi7Takuya Takahashi8Hirokazu Tsuji9Hirokazu Tsuji10Koji Nomoto11Koji Nomoto12Ravinder Nagpal13Chongxin Wang14Satoru Nagata15Satoru Nagata16Yuichiro Yamashiro17Juntendo University School of Health and Sports ScienceChiba, JapanJuntendo University School of Health and Sports ScienceChiba, JapanJuntendo University School of Health and Sports ScienceChiba, JapanJuntendo University School of Health and Sports ScienceChiba, JapanJuntendo University School of Health and Sports ScienceChiba, JapanYakult Central InstituteTokyo, JapanProbiotics Research Laboratory, Juntendo University Graduate School of MedicineTokyo, JapanYakult Central InstituteTokyo, JapanProbiotics Research Laboratory, Juntendo University Graduate School of MedicineTokyo, JapanYakult Central InstituteTokyo, JapanProbiotics Research Laboratory, Juntendo University Graduate School of MedicineTokyo, JapanYakult Central InstituteTokyo, JapanProbiotics Research Laboratory, Juntendo University Graduate School of MedicineTokyo, JapanProbiotics Research Laboratory, Juntendo University Graduate School of MedicineTokyo, JapanProbiotics Research Laboratory, Juntendo University Graduate School of MedicineTokyo, JapanProbiotics Research Laboratory, Juntendo University Graduate School of MedicineTokyo, JapanDepartment of Pediatrics, Tokyo Women’s Medical UniversityTokyo, JapanProbiotics Research Laboratory, Juntendo University Graduate School of MedicineTokyo, JapanHuman intestinal microbiota are influenced by various factors viz. diet, environment, age, gender, geographical, and socioeconomic situation, etc. among which diet has the most profound impact. However, studies investigating this impact have mostly included subjects from diverse geographic/socioeconomic backgrounds and hence the precise effects of dietary factors on gut microbiota composition remain largely confounded. Herein, with an aim to evaluate the association between dietary habits, specifically yogurt consumption, and the gut microbiota in healthy young adults sharing similar age, lifestyle routine, geographical setting, etc., we conducted a cross-sectional study wherein 293 collegiate freshmen answered a questionnaire about their frequency of yogurt consumption over the last 2 months and provided stool specimens for microbiota analysis. Fecal microbiota were analyzed by highly sensitive reverse-transcription-quantitative-PCR assays targeting bacterial 16S rRNA molecules. Fecal organic acids were measured by HPLC. Overall, the gut microbiota were predominated (97.1 ± 8.6%) by Clostridium coccoides group, Clostridium leptum subgroup, Bacteroides fragilis group, Bifidobacterium and Atopobium cluster. Interestingly, after adjusting the data for yogurt consumption, females were found to have higher total bacterial (P = 0.013) and Bifidobacterium (P = 0.046) count and fecal pH (P = 0.007) and lower fecal concentration of total organic acids (P = 0.030), succinic acid (P = 0.007) and formic acid (P = 0.046) as compared to males. Altogether, yogurt consumption showed positive linear association with Lactobacillus and Lactobacillus gasseri subgroup in both male and female subjects; however, several gender-specific disparities were also detected in this yogurt-microbiota association. Yogurt consumption demonstrated a negative association with L. sakei subgroup, Enterobacteriaceae and Staphylococcus in males but shared a positive association with L. casei subgroup and succinic acid in female subjects. The study manifests the association between yogurt consumption and gut microbiota in a healthy homogeneous cohort and show how this association can differ by host gender. The findings should be helpful for prospective studies investigating the diet–microbiome interaction in human health and disease.http://journal.frontiersin.org/article/10.3389/fmicb.2017.00847/fulldietgenderyogurtprobioticsgut bacteriaLactobacillus |
| spellingShingle | Yoshio Suzuki Keiichi Ikeda Kazuhiko Sakuma Sachio Kawai Keisuke Sawaki Takashi Asahara Takashi Asahara Takuya Takahashi Takuya Takahashi Hirokazu Tsuji Hirokazu Tsuji Koji Nomoto Koji Nomoto Ravinder Nagpal Chongxin Wang Satoru Nagata Satoru Nagata Yuichiro Yamashiro Association between Yogurt Consumption and Intestinal Microbiota in Healthy Young Adults Differs by Host Gender Frontiers in Microbiology diet gender yogurt probiotics gut bacteria Lactobacillus |
| title | Association between Yogurt Consumption and Intestinal Microbiota in Healthy Young Adults Differs by Host Gender |
| title_full | Association between Yogurt Consumption and Intestinal Microbiota in Healthy Young Adults Differs by Host Gender |
| title_fullStr | Association between Yogurt Consumption and Intestinal Microbiota in Healthy Young Adults Differs by Host Gender |
| title_full_unstemmed | Association between Yogurt Consumption and Intestinal Microbiota in Healthy Young Adults Differs by Host Gender |
| title_short | Association between Yogurt Consumption and Intestinal Microbiota in Healthy Young Adults Differs by Host Gender |
| title_sort | association between yogurt consumption and intestinal microbiota in healthy young adults differs by host gender |
| topic | diet gender yogurt probiotics gut bacteria Lactobacillus |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.00847/full |
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