Antimalarial activity of Toona ciliata MJ Roem aqueous methanolic leaf extract and its antioxidant and phytochemical properties
Background and aim: Malaria is a global health issue causing substantial morbidity and mortality. Screening of various traditionally important medicinal plants is a key source for the discovery of new antimalarials. We evaluated the antimalarial and antioxidant activities, and performed detailed phy...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Journal of Traditional and Complementary Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2225411023000597 |
| _version_ | 1797643594656382976 |
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| author | Nalini Singh Aditi Chatterjee Wahengbam Kabita Chanu Pradeep Mini Vaishalli Chingakham Brajakishor Singh Viswanathan Arun Nagaraj |
| author_facet | Nalini Singh Aditi Chatterjee Wahengbam Kabita Chanu Pradeep Mini Vaishalli Chingakham Brajakishor Singh Viswanathan Arun Nagaraj |
| author_sort | Nalini Singh |
| collection | DOAJ |
| description | Background and aim: Malaria is a global health issue causing substantial morbidity and mortality. Screening of various traditionally important medicinal plants is a key source for the discovery of new antimalarials. We evaluated the antimalarial and antioxidant activities, and performed detailed phytochemical analyses of Toona ciliata MJ Roem aqueous methanolic leaf extract (TcMLE). Experimental procedures: In vitro antiplasmodial studies in Plasmodium falciparum (Pf) 3D7 and PfCam3.IR539T strains were performed by [3H]-hypoxanthine uptake assays. In vitro cytotoxicity in HeLa and HEK293T cell lines was evaluated using MTT assays. Hemolysis assay was performed using RBCs. Phytochemical analysis by GC-MS and in vitro antioxidant studies by DPPH and ABTS assays were performed. In vivo antimalarial studies in Pb-infected mice were carried out using Rane's test and Peters' 4-day test. Results and conclusions: TcMLE showed significant in vitro antioxidant activity and had phytochemicals reported for antimalarial activity. In vitro studies showed prominent antiplasmodial activity against Pf3D7 strain (IC50 ∼22 μg/ml) and PfCam3. IR539Tstrain (IC50 value ∼43 μg/ml). In vitro cytotoxicity studies, in vitro hemolytic assays, and in vivo acute toxicity studies further suggested that TcMLE is nontoxic. In vivo antimalarial studies using Rane's test showed a significant decrease in parasitemia by ∼70% at 1200 mg/kg doses and delayed the mortality of mice by ∼10–14 days. Peters' 4-day test also showed a similar pattern. The present study demonstrated the antimalarial potential of TcMLE. These findings deliver a platform for further studies to identify the active components of TcMLE and discover new antimalarials. |
| first_indexed | 2024-03-11T14:17:09Z |
| format | Article |
| id | doaj.art-54caeab256cd4e45ab9cea69248bbaf6 |
| institution | Directory Open Access Journal |
| issn | 2225-4110 |
| language | English |
| last_indexed | 2024-03-11T14:17:09Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional and Complementary Medicine |
| spelling | doaj.art-54caeab256cd4e45ab9cea69248bbaf62023-11-01T04:47:18ZengElsevierJournal of Traditional and Complementary Medicine2225-41102023-11-01136550560Antimalarial activity of Toona ciliata MJ Roem aqueous methanolic leaf extract and its antioxidant and phytochemical propertiesNalini Singh0Aditi Chatterjee1Wahengbam Kabita Chanu2Pradeep Mini Vaishalli3Chingakham Brajakishor Singh4Viswanathan Arun Nagaraj5Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, 751023, Odisha, IndiaInfectious Disease Biology, Institute of Life Sciences, Bhubaneswar, 751023, Odisha, India; School of Biotechnology, Kalinga Institute of Industrial Technology, Bhubaneswar, 751024, Odisha, IndiaPlant Bioresources Division, Institute of Bioresources and Sustainable Development, Imphal, 795001, Manipur, IndiaInfectious Disease Biology, Institute of Life Sciences, Bhubaneswar, 751023, Odisha, IndiaPlant Bioresources Division, Institute of Bioresources and Sustainable Development, Imphal, 795001, Manipur, India; Corresponding author.Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, 751023, Odisha, India; Corresponding author.Background and aim: Malaria is a global health issue causing substantial morbidity and mortality. Screening of various traditionally important medicinal plants is a key source for the discovery of new antimalarials. We evaluated the antimalarial and antioxidant activities, and performed detailed phytochemical analyses of Toona ciliata MJ Roem aqueous methanolic leaf extract (TcMLE). Experimental procedures: In vitro antiplasmodial studies in Plasmodium falciparum (Pf) 3D7 and PfCam3.IR539T strains were performed by [3H]-hypoxanthine uptake assays. In vitro cytotoxicity in HeLa and HEK293T cell lines was evaluated using MTT assays. Hemolysis assay was performed using RBCs. Phytochemical analysis by GC-MS and in vitro antioxidant studies by DPPH and ABTS assays were performed. In vivo antimalarial studies in Pb-infected mice were carried out using Rane's test and Peters' 4-day test. Results and conclusions: TcMLE showed significant in vitro antioxidant activity and had phytochemicals reported for antimalarial activity. In vitro studies showed prominent antiplasmodial activity against Pf3D7 strain (IC50 ∼22 μg/ml) and PfCam3. IR539Tstrain (IC50 value ∼43 μg/ml). In vitro cytotoxicity studies, in vitro hemolytic assays, and in vivo acute toxicity studies further suggested that TcMLE is nontoxic. In vivo antimalarial studies using Rane's test showed a significant decrease in parasitemia by ∼70% at 1200 mg/kg doses and delayed the mortality of mice by ∼10–14 days. Peters' 4-day test also showed a similar pattern. The present study demonstrated the antimalarial potential of TcMLE. These findings deliver a platform for further studies to identify the active components of TcMLE and discover new antimalarials.http://www.sciencedirect.com/science/article/pii/S2225411023000597Medicinal plantsAntiplasmodialHemolyticCytotoxicityAcute toxicity |
| spellingShingle | Nalini Singh Aditi Chatterjee Wahengbam Kabita Chanu Pradeep Mini Vaishalli Chingakham Brajakishor Singh Viswanathan Arun Nagaraj Antimalarial activity of Toona ciliata MJ Roem aqueous methanolic leaf extract and its antioxidant and phytochemical properties Journal of Traditional and Complementary Medicine Medicinal plants Antiplasmodial Hemolytic Cytotoxicity Acute toxicity |
| title | Antimalarial activity of Toona ciliata MJ Roem aqueous methanolic leaf extract and its antioxidant and phytochemical properties |
| title_full | Antimalarial activity of Toona ciliata MJ Roem aqueous methanolic leaf extract and its antioxidant and phytochemical properties |
| title_fullStr | Antimalarial activity of Toona ciliata MJ Roem aqueous methanolic leaf extract and its antioxidant and phytochemical properties |
| title_full_unstemmed | Antimalarial activity of Toona ciliata MJ Roem aqueous methanolic leaf extract and its antioxidant and phytochemical properties |
| title_short | Antimalarial activity of Toona ciliata MJ Roem aqueous methanolic leaf extract and its antioxidant and phytochemical properties |
| title_sort | antimalarial activity of toona ciliata mj roem aqueous methanolic leaf extract and its antioxidant and phytochemical properties |
| topic | Medicinal plants Antiplasmodial Hemolytic Cytotoxicity Acute toxicity |
| url | http://www.sciencedirect.com/science/article/pii/S2225411023000597 |
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