Artificial fertilization and generating families for a selective breeding programme of large yellow croaker (Larimichthys crocea)
Abstract Large yellow croaker is an important marine aquaculture species in China. The aim was to determine an appropriate protocol of artificial fertilization for family construction in the breeding programme based on two trials. In trial 1, luteinizing hormone-releasing hormone A3 (LHRHA3) was inj...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Islamic Azad University
2017-04-01
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| Series: | International Aquatic Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1007/s40071-017-0164-3 |
| _version_ | 1818172213863383040 |
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| author | Xinxiu Yu Changwen Wu Hans Magnus Gjøen |
| author_facet | Xinxiu Yu Changwen Wu Hans Magnus Gjøen |
| author_sort | Xinxiu Yu |
| collection | DOAJ |
| description | Abstract Large yellow croaker is an important marine aquaculture species in China. The aim was to determine an appropriate protocol of artificial fertilization for family construction in the breeding programme based on two trials. In trial 1, luteinizing hormone-releasing hormone A3 (LHRHA3) was injected once, with a dosage of 2 μg/kg for females and 1 μg/kg for males. The latency time was in the range of 30–34 h. The maturation stage was checked by extracting a few eggs with a Pasteur pipette. The fertilization rate and hatching rate were 27 and 52%, respectively. The percentage of females with spawning difficulties was 30%. In trial 2, the females were injected LHRHA3 twice: with a first dose of 0.8 μg/kg and a second dose of 2 μg/kg, at an interval of 10 h, whereas the males were still injected once. The latency time was in the range of 29.5–35 h, determined by only observing courtship behaviour of males. The females with spawning difficulties decreased to 10%, and the fertilization rate and hatching rate also improved to 41 and 62%, respectively. |
| first_indexed | 2024-12-11T19:09:03Z |
| format | Article |
| id | doaj.art-54cbecfd35ba40e0b4058989ff2e5b67 |
| institution | Directory Open Access Journal |
| issn | 2008-4935 2008-6970 |
| language | English |
| last_indexed | 2024-12-11T19:09:03Z |
| publishDate | 2017-04-01 |
| publisher | Islamic Azad University |
| record_format | Article |
| series | International Aquatic Research |
| spelling | doaj.art-54cbecfd35ba40e0b4058989ff2e5b672022-12-22T00:53:49ZengIslamic Azad UniversityInternational Aquatic Research2008-49352008-69702017-04-019216116710.1007/s40071-017-0164-3Artificial fertilization and generating families for a selective breeding programme of large yellow croaker (Larimichthys crocea)Xinxiu Yu0Changwen Wu1Hans Magnus Gjøen2Department of Animal and Aquacultural Sciences, Norwegian University of Life SciencesNational Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean UniversityDepartment of Animal and Aquacultural Sciences, Norwegian University of Life SciencesAbstract Large yellow croaker is an important marine aquaculture species in China. The aim was to determine an appropriate protocol of artificial fertilization for family construction in the breeding programme based on two trials. In trial 1, luteinizing hormone-releasing hormone A3 (LHRHA3) was injected once, with a dosage of 2 μg/kg for females and 1 μg/kg for males. The latency time was in the range of 30–34 h. The maturation stage was checked by extracting a few eggs with a Pasteur pipette. The fertilization rate and hatching rate were 27 and 52%, respectively. The percentage of females with spawning difficulties was 30%. In trial 2, the females were injected LHRHA3 twice: with a first dose of 0.8 μg/kg and a second dose of 2 μg/kg, at an interval of 10 h, whereas the males were still injected once. The latency time was in the range of 29.5–35 h, determined by only observing courtship behaviour of males. The females with spawning difficulties decreased to 10%, and the fertilization rate and hatching rate also improved to 41 and 62%, respectively.http://link.springer.com/article/10.1007/s40071-017-0164-3Large yellow croakerArtificial fertilizationHormone injectionsLatency time |
| spellingShingle | Xinxiu Yu Changwen Wu Hans Magnus Gjøen Artificial fertilization and generating families for a selective breeding programme of large yellow croaker (Larimichthys crocea) International Aquatic Research Large yellow croaker Artificial fertilization Hormone injections Latency time |
| title | Artificial fertilization and generating families for a selective breeding programme of large yellow croaker (Larimichthys crocea) |
| title_full | Artificial fertilization and generating families for a selective breeding programme of large yellow croaker (Larimichthys crocea) |
| title_fullStr | Artificial fertilization and generating families for a selective breeding programme of large yellow croaker (Larimichthys crocea) |
| title_full_unstemmed | Artificial fertilization and generating families for a selective breeding programme of large yellow croaker (Larimichthys crocea) |
| title_short | Artificial fertilization and generating families for a selective breeding programme of large yellow croaker (Larimichthys crocea) |
| title_sort | artificial fertilization and generating families for a selective breeding programme of large yellow croaker larimichthys crocea |
| topic | Large yellow croaker Artificial fertilization Hormone injections Latency time |
| url | http://link.springer.com/article/10.1007/s40071-017-0164-3 |
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