The effect of oral and intraurethral trimetazidine use on urethral healing
Objective(s): We aimed to study the effect of trimetazidine (TMZ) on urethral wound repair. Materials and Methods: A total of 52 male rats were used; 8 groups were formed: 1-week and 3-week control (C1,C3), sham (S1, S3), oral (OT1, OT3), and intraurethral TMZ (IUT1, IUT3) groups. Serum and urine to...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2016-09-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/article_7592_dd2ecf4a40f637ea35f5d633f431c69b.pdf |
| _version_ | 1818777175134830592 |
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| author | Ali Ayyildiz Sema Nur Ayyildiz Erdal Benli Havva Erdem Selma Cirrik Tevfik Noyan R. Cankon Germiyanoglu Abdullah Çirakoglu |
| author_facet | Ali Ayyildiz Sema Nur Ayyildiz Erdal Benli Havva Erdem Selma Cirrik Tevfik Noyan R. Cankon Germiyanoglu Abdullah Çirakoglu |
| author_sort | Ali Ayyildiz |
| collection | DOAJ |
| description | Objective(s): We aimed to study the effect of trimetazidine (TMZ) on urethral wound repair.
Materials and Methods: A total of 52 male rats were used; 8 groups were formed: 1-week and 3-week control (C1,C3), sham (S1, S3), oral (OT1, OT3), and intraurethral TMZ (IUT1, IUT3) groups. Serum and urine total antioxidant capacity (TAC), total oxidant capacity (TOC), and 8-hydroxy-deoxy-guanosine (8-OHdG) were studied. Hematoxyline-Eosin was used for the histopathological study. In addition, tumor necrosis factor alpha (TNF- α), interleukin 1α, and β levels were compared across groups by an immunohistochemical method.
Results: There were significant differences between C3 and IUT3, OT3 and IUT3 with respect to serum TAC in 3-week groups (P=0.013; P =0.001). Serum TOC levels were significantly different between C3 and IUT3; S3 and OT3; and OT3 and IUT3 groups (P =0.024; P =0.019; P =0.000, respectively). Serum 8-OHdG levels were significantly different between C3 and OT3 groups (P=0.033). In the immunohistochemical examination, C1 and OT1; C1 and IUT1; and S1, S3, OT1, OT3, IUT1 groups were significantly different with respect to IL-1β staining (P=0.007; P =0.000; P=0.009), while there was a significant difference between C3 and S3 with respect to IL-1β (P =0.000).
Conclusion: TMZ increased urinary total oxidant level; while increasing serum TAC levels in the long-term. It also reduced serum TAC levels in urethral use and caused an increase in serum TOC levels with minimal effects on DNA injury and repair. No effect was detected on IL1 α and TNF, but partially reduced the effect on IL-1 β levels. |
| first_indexed | 2024-12-18T11:24:39Z |
| format | Article |
| id | doaj.art-54cc0eb2890f416682becade206ef5fa |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-18T11:24:39Z |
| publishDate | 2016-09-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-54cc0eb2890f416682becade206ef5fa2022-12-21T21:09:45ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742016-09-0119993293910.22038/ijbms.2016.75927592The effect of oral and intraurethral trimetazidine use on urethral healingAli Ayyildiz0Sema Nur Ayyildiz1Erdal Benli2Havva Erdem3Selma Cirrik4Tevfik Noyan5R. Cankon Germiyanoglu6Abdullah Çirakoglu7Ordu University Medical Faculty, Urology Department, Ordu, TurkeyOrdu University Medical Faculty, Clinical Biochemistry Department, Ordu, TurkeyOrdu University Medical Faculty, Urology Department, Ordu, TurkeyOrdu University Medical Faculty, Pathology Department, Ordu, TurkeyOrdu University Medical Faculty, Pathology Department, Ordu, TurkeyOrdu University Medical Faculty, Clinical Biochemistry Department, Ordu, TurkeyOndokuz Mayıs University Medical Faculty, Urology Department, Samsun, TurkeyOrdu University Medical Faculty, Urology Department, Ordu, TurkeyObjective(s): We aimed to study the effect of trimetazidine (TMZ) on urethral wound repair. Materials and Methods: A total of 52 male rats were used; 8 groups were formed: 1-week and 3-week control (C1,C3), sham (S1, S3), oral (OT1, OT3), and intraurethral TMZ (IUT1, IUT3) groups. Serum and urine total antioxidant capacity (TAC), total oxidant capacity (TOC), and 8-hydroxy-deoxy-guanosine (8-OHdG) were studied. Hematoxyline-Eosin was used for the histopathological study. In addition, tumor necrosis factor alpha (TNF- α), interleukin 1α, and β levels were compared across groups by an immunohistochemical method. Results: There were significant differences between C3 and IUT3, OT3 and IUT3 with respect to serum TAC in 3-week groups (P=0.013; P =0.001). Serum TOC levels were significantly different between C3 and IUT3; S3 and OT3; and OT3 and IUT3 groups (P =0.024; P =0.019; P =0.000, respectively). Serum 8-OHdG levels were significantly different between C3 and OT3 groups (P=0.033). In the immunohistochemical examination, C1 and OT1; C1 and IUT1; and S1, S3, OT1, OT3, IUT1 groups were significantly different with respect to IL-1β staining (P=0.007; P =0.000; P=0.009), while there was a significant difference between C3 and S3 with respect to IL-1β (P =0.000). Conclusion: TMZ increased urinary total oxidant level; while increasing serum TAC levels in the long-term. It also reduced serum TAC levels in urethral use and caused an increase in serum TOC levels with minimal effects on DNA injury and repair. No effect was detected on IL1 α and TNF, but partially reduced the effect on IL-1 β levels.http://ijbms.mums.ac.ir/article_7592_dd2ecf4a40f637ea35f5d633f431c69b.pdfOxidative stressTrimetazidineUrethral healingUrethral injury |
| spellingShingle | Ali Ayyildiz Sema Nur Ayyildiz Erdal Benli Havva Erdem Selma Cirrik Tevfik Noyan R. Cankon Germiyanoglu Abdullah Çirakoglu The effect of oral and intraurethral trimetazidine use on urethral healing Iranian Journal of Basic Medical Sciences Oxidative stress Trimetazidine Urethral healing Urethral injury |
| title | The effect of oral and intraurethral trimetazidine use on urethral healing |
| title_full | The effect of oral and intraurethral trimetazidine use on urethral healing |
| title_fullStr | The effect of oral and intraurethral trimetazidine use on urethral healing |
| title_full_unstemmed | The effect of oral and intraurethral trimetazidine use on urethral healing |
| title_short | The effect of oral and intraurethral trimetazidine use on urethral healing |
| title_sort | effect of oral and intraurethral trimetazidine use on urethral healing |
| topic | Oxidative stress Trimetazidine Urethral healing Urethral injury |
| url | http://ijbms.mums.ac.ir/article_7592_dd2ecf4a40f637ea35f5d633f431c69b.pdf |
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