Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes
We describe a method for culturing over 90% pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2003-03-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000200017 |
| _version_ | 1827831666008129536 |
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| author | Omar A Saldarriaga Jaime I Velásquez Jorge E Ossa Maria T Rugeles |
| author_facet | Omar A Saldarriaga Jaime I Velásquez Jorge E Ossa Maria T Rugeles |
| author_sort | Omar A Saldarriaga |
| collection | DOAJ |
| description | We describe a method for culturing over 90% pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before. |
| first_indexed | 2024-03-12T04:57:36Z |
| format | Article |
| id | doaj.art-54cc376293ee4acfa525c0e8ba74a5b7 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T04:57:36Z |
| publishDate | 2003-03-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-54cc376293ee4acfa525c0e8ba74a5b72023-09-03T09:14:42ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602003-03-0198226927110.1590/S0074-02762003000200017Standardization of bovine macrophage monolayers and isolation and culture of trypanosomesOmar A SaldarriagaJaime I VelásquezJorge E OssaMaria T RugelesWe describe a method for culturing over 90% pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000200017macrophagesbovinetrypanosomes |
| spellingShingle | Omar A Saldarriaga Jaime I Velásquez Jorge E Ossa Maria T Rugeles Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes Memorias do Instituto Oswaldo Cruz macrophages bovine trypanosomes |
| title | Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes |
| title_full | Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes |
| title_fullStr | Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes |
| title_full_unstemmed | Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes |
| title_short | Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes |
| title_sort | standardization of bovine macrophage monolayers and isolation and culture of trypanosomes |
| topic | macrophages bovine trypanosomes |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000200017 |
| work_keys_str_mv | AT omarasaldarriaga standardizationofbovinemacrophagemonolayersandisolationandcultureoftrypanosomes AT jaimeivelasquez standardizationofbovinemacrophagemonolayersandisolationandcultureoftrypanosomes AT jorgeeossa standardizationofbovinemacrophagemonolayersandisolationandcultureoftrypanosomes AT mariatrugeles standardizationofbovinemacrophagemonolayersandisolationandcultureoftrypanosomes |