Two single nucleotide polymorphisms sites in α1-AT gene and their association with somatic cell score in Chinese Holstein cows
Abstract Background Alpha 1-antitrypsin (α1-AT) may affect the susceptibility of mastitis in dairy cattle for its possible role in the protection of lactoferrin from proteolytic degradation in the mammary. Milk somatic cell score (SCS) is a logarithmic transformation of the milk somatic cell count w...
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| Format: | Article |
| Language: | English |
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Aristotle University of Thessaloniki
2017-04-01
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| Series: | Journal of Biological Research - Thessaloniki |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40709-017-0065-z |
| _version_ | 1828303393129496576 |
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| author | Xiao-Fei Guo Wen-Ping Hu Xian-Zheng Lang Qiu-Ling Li Xiang-Yu Wang Ran Di Qiu-Yue Liu Xiao-Lin Liu Yong-Fu An Ming-Xing Chu |
| author_facet | Xiao-Fei Guo Wen-Ping Hu Xian-Zheng Lang Qiu-Ling Li Xiang-Yu Wang Ran Di Qiu-Yue Liu Xiao-Lin Liu Yong-Fu An Ming-Xing Chu |
| author_sort | Xiao-Fei Guo |
| collection | DOAJ |
| description | Abstract Background Alpha 1-antitrypsin (α1-AT) may affect the susceptibility of mastitis in dairy cattle for its possible role in the protection of lactoferrin from proteolytic degradation in the mammary. Milk somatic cell score (SCS) is a logarithmic transformation of the milk somatic cell count widely used as an index to evaluate mastitis. To study the relationships of α1-AT gene and SCS in Chinese Holstein cows, methods of PCR-SSCP, DNA sequencing, PCR-RFLP, and CRS-PCR technologies were used to detect single nucleotide polymorphisms sites in α1-AT gene. Results Two polymorphic sites at G5503A and G5746C of α1-AT gene were found. AA (0.3633), AB (0.4644) and BB (0.1723) genotypes were detected at G5503A site, CC (0.3483), CD (0.4906) and DD (0.1611) genotypes were found at G5746C in Chinese Holstein cows. Least squares mean of SCS for individuals with BB genotype was significantly lower than that with AA and AB genotype (p < 0.01), and that with AB genotype was significantly lower than that with AA (p < 0.05). There was no significant difference among individuals with CC, CD and DD genotypes (p > 0.05). Least squares mean of SCS for individuals with BBDD genotype combination were significantly lower than those with AACC and AACD (p < 0.05). Conclusions Statistical analysis indicated that B allele and BBDD genotype combination of α1-AT can improve mastitis resistance in dairy cattle. |
| first_indexed | 2024-04-13T13:53:15Z |
| format | Article |
| id | doaj.art-54cdb782d4194f9bb852927de97f020a |
| institution | Directory Open Access Journal |
| issn | 2241-5793 |
| language | English |
| last_indexed | 2024-04-13T13:53:15Z |
| publishDate | 2017-04-01 |
| publisher | Aristotle University of Thessaloniki |
| record_format | Article |
| series | Journal of Biological Research - Thessaloniki |
| spelling | doaj.art-54cdb782d4194f9bb852927de97f020a2022-12-22T02:44:17ZengAristotle University of ThessalonikiJournal of Biological Research - Thessaloniki2241-57932017-04-012411810.1186/s40709-017-0065-zTwo single nucleotide polymorphisms sites in α1-AT gene and their association with somatic cell score in Chinese Holstein cowsXiao-Fei Guo0Wen-Ping Hu1Xian-Zheng Lang2Qiu-Ling Li3Xiang-Yu Wang4Ran Di5Qiu-Yue Liu6Xiao-Lin Liu7Yong-Fu An8Ming-Xing Chu9Key Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural SciencesKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural SciencesCollege of Animal Science and Technology, Northwest A &F UniversityLife Sciences College, Langfang Teachers UniversityKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural SciencesKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural SciencesKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural SciencesCollege of Animal Science and Technology, Northwest A &F UniversityHebei Animal Science and Veterinary Medicine InstituteKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural SciencesAbstract Background Alpha 1-antitrypsin (α1-AT) may affect the susceptibility of mastitis in dairy cattle for its possible role in the protection of lactoferrin from proteolytic degradation in the mammary. Milk somatic cell score (SCS) is a logarithmic transformation of the milk somatic cell count widely used as an index to evaluate mastitis. To study the relationships of α1-AT gene and SCS in Chinese Holstein cows, methods of PCR-SSCP, DNA sequencing, PCR-RFLP, and CRS-PCR technologies were used to detect single nucleotide polymorphisms sites in α1-AT gene. Results Two polymorphic sites at G5503A and G5746C of α1-AT gene were found. AA (0.3633), AB (0.4644) and BB (0.1723) genotypes were detected at G5503A site, CC (0.3483), CD (0.4906) and DD (0.1611) genotypes were found at G5746C in Chinese Holstein cows. Least squares mean of SCS for individuals with BB genotype was significantly lower than that with AA and AB genotype (p < 0.01), and that with AB genotype was significantly lower than that with AA (p < 0.05). There was no significant difference among individuals with CC, CD and DD genotypes (p > 0.05). Least squares mean of SCS for individuals with BBDD genotype combination were significantly lower than those with AACC and AACD (p < 0.05). Conclusions Statistical analysis indicated that B allele and BBDD genotype combination of α1-AT can improve mastitis resistance in dairy cattle.http://link.springer.com/article/10.1186/s40709-017-0065-zSNPα1-AT genePolymorphismSomatic cell scoreChinese Holstein cows |
| spellingShingle | Xiao-Fei Guo Wen-Ping Hu Xian-Zheng Lang Qiu-Ling Li Xiang-Yu Wang Ran Di Qiu-Yue Liu Xiao-Lin Liu Yong-Fu An Ming-Xing Chu Two single nucleotide polymorphisms sites in α1-AT gene and their association with somatic cell score in Chinese Holstein cows Journal of Biological Research - Thessaloniki SNP α1-AT gene Polymorphism Somatic cell score Chinese Holstein cows |
| title | Two single nucleotide polymorphisms sites in α1-AT gene and their association with somatic cell score in Chinese Holstein cows |
| title_full | Two single nucleotide polymorphisms sites in α1-AT gene and their association with somatic cell score in Chinese Holstein cows |
| title_fullStr | Two single nucleotide polymorphisms sites in α1-AT gene and their association with somatic cell score in Chinese Holstein cows |
| title_full_unstemmed | Two single nucleotide polymorphisms sites in α1-AT gene and their association with somatic cell score in Chinese Holstein cows |
| title_short | Two single nucleotide polymorphisms sites in α1-AT gene and their association with somatic cell score in Chinese Holstein cows |
| title_sort | two single nucleotide polymorphisms sites in α1 at gene and their association with somatic cell score in chinese holstein cows |
| topic | SNP α1-AT gene Polymorphism Somatic cell score Chinese Holstein cows |
| url | http://link.springer.com/article/10.1186/s40709-017-0065-z |
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