Serum metabolism alteration behind different etiology, diagnosis, and prognosis of disorders of consciousness
Abstract Background Patients with disorders of consciousness (DoC) exhibit varied revival outcomes based on different etiologies and diagnoses, the mechanisms of which remain largely unknown. The fluctuating clinical presentations in DoC pose challenges in accurately assessing consciousness levels a...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | Chinese Neurosurgical Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41016-024-00365-4 |
| _version_ | 1827284447589826560 |
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| author | Qianqian Ge Hezhen Lu Xiaoli Geng Xueling Chen Xiaoyan Liu Haidan Sun Zhengguang Guo Jiameng Sun Feng Qi Xia Niu Aiwei Wang Jianghong He Wei Sun Long Xu |
| author_facet | Qianqian Ge Hezhen Lu Xiaoli Geng Xueling Chen Xiaoyan Liu Haidan Sun Zhengguang Guo Jiameng Sun Feng Qi Xia Niu Aiwei Wang Jianghong He Wei Sun Long Xu |
| author_sort | Qianqian Ge |
| collection | DOAJ |
| description | Abstract Background Patients with disorders of consciousness (DoC) exhibit varied revival outcomes based on different etiologies and diagnoses, the mechanisms of which remain largely unknown. The fluctuating clinical presentations in DoC pose challenges in accurately assessing consciousness levels and prognoses, often leading to misdiagnoses. There is an urgent need for a deeper understanding of the physiological changes in DoC and the development of objective diagnostic and prognostic biomarkers to improve treatment guidance. Methods To explore biomarkers and understand the biological processes, we conducted a comprehensive untargeted metabolomic analysis on serum samples from 48 patients with DoC. Patients were categorized based on etiology (TBI vs. non-TBI), CRS-R scores, and prognosis. Advanced analytical techniques, including PCA and OPLS-DA models, were employed to identify differential metabolites. Results Our analysis revealed a distinct separation in metabolomic profiles among the different groups. The primary differential metabolites distinguishing patients with varying etiologies were predominantly phospholipids, with a notable decrease in glycerophospholipids observed in the TBI group. Patients with higher CRS-R scores exhibited a pattern of impaired carbohydrate metabolism coupled with enhanced lipid metabolism. Notably, serum concentrations of both LysoPE and PE were reduced in patients with improved outcomes, suggesting their potential as prognostic biomarkers. Conclusions Our study underscores the critical role of phospholipid metabolism in the brain’s metabolic alterations in patients with DoC. It identifies key biomarkers for diagnosis and prognosis, offering insights that could lead to novel therapeutic targets. These findings highlight the value of metabolomic profiling in understanding and potentially treating DoC. |
| first_indexed | 2024-04-24T09:56:35Z |
| format | Article |
| id | doaj.art-54d1037f34d44edb9e07e515f0a3ec0c |
| institution | Directory Open Access Journal |
| issn | 2057-4967 |
| language | English |
| last_indexed | 2024-04-24T09:56:35Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Chinese Neurosurgical Journal |
| spelling | doaj.art-54d1037f34d44edb9e07e515f0a3ec0c2024-04-14T11:08:11ZengBMCChinese Neurosurgical Journal2057-49672024-04-0110111210.1186/s41016-024-00365-4Serum metabolism alteration behind different etiology, diagnosis, and prognosis of disorders of consciousnessQianqian Ge0Hezhen Lu1Xiaoli Geng2Xueling Chen3Xiaoyan Liu4Haidan Sun5Zhengguang Guo6Jiameng Sun7Feng Qi8Xia Niu9Aiwei Wang10Jianghong He11Wei Sun12Long Xu13Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical UniversityChina-Japan Union Hospital of Jilin UniversityDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical UniversityDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical UniversityCore Instrument Facility, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical CollegeCore Instrument Facility, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical CollegeCore Instrument Facility, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical CollegeCore Instrument Facility, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical CollegeCore Instrument Facility, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical CollegeCore Instrument Facility, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical CollegeCore Instrument Facility, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical CollegeDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical UniversityCore Instrument Facility, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical CollegeDepartment of Neurosurgery, Beijing Tiantan Hospital, Capital Medical UniversityAbstract Background Patients with disorders of consciousness (DoC) exhibit varied revival outcomes based on different etiologies and diagnoses, the mechanisms of which remain largely unknown. The fluctuating clinical presentations in DoC pose challenges in accurately assessing consciousness levels and prognoses, often leading to misdiagnoses. There is an urgent need for a deeper understanding of the physiological changes in DoC and the development of objective diagnostic and prognostic biomarkers to improve treatment guidance. Methods To explore biomarkers and understand the biological processes, we conducted a comprehensive untargeted metabolomic analysis on serum samples from 48 patients with DoC. Patients were categorized based on etiology (TBI vs. non-TBI), CRS-R scores, and prognosis. Advanced analytical techniques, including PCA and OPLS-DA models, were employed to identify differential metabolites. Results Our analysis revealed a distinct separation in metabolomic profiles among the different groups. The primary differential metabolites distinguishing patients with varying etiologies were predominantly phospholipids, with a notable decrease in glycerophospholipids observed in the TBI group. Patients with higher CRS-R scores exhibited a pattern of impaired carbohydrate metabolism coupled with enhanced lipid metabolism. Notably, serum concentrations of both LysoPE and PE were reduced in patients with improved outcomes, suggesting their potential as prognostic biomarkers. Conclusions Our study underscores the critical role of phospholipid metabolism in the brain’s metabolic alterations in patients with DoC. It identifies key biomarkers for diagnosis and prognosis, offering insights that could lead to novel therapeutic targets. These findings highlight the value of metabolomic profiling in understanding and potentially treating DoC.https://doi.org/10.1186/s41016-024-00365-4Disorders of consciousnessSerum biomarkersUntargeted metabolomic analysis |
| spellingShingle | Qianqian Ge Hezhen Lu Xiaoli Geng Xueling Chen Xiaoyan Liu Haidan Sun Zhengguang Guo Jiameng Sun Feng Qi Xia Niu Aiwei Wang Jianghong He Wei Sun Long Xu Serum metabolism alteration behind different etiology, diagnosis, and prognosis of disorders of consciousness Chinese Neurosurgical Journal Disorders of consciousness Serum biomarkers Untargeted metabolomic analysis |
| title | Serum metabolism alteration behind different etiology, diagnosis, and prognosis of disorders of consciousness |
| title_full | Serum metabolism alteration behind different etiology, diagnosis, and prognosis of disorders of consciousness |
| title_fullStr | Serum metabolism alteration behind different etiology, diagnosis, and prognosis of disorders of consciousness |
| title_full_unstemmed | Serum metabolism alteration behind different etiology, diagnosis, and prognosis of disorders of consciousness |
| title_short | Serum metabolism alteration behind different etiology, diagnosis, and prognosis of disorders of consciousness |
| title_sort | serum metabolism alteration behind different etiology diagnosis and prognosis of disorders of consciousness |
| topic | Disorders of consciousness Serum biomarkers Untargeted metabolomic analysis |
| url | https://doi.org/10.1186/s41016-024-00365-4 |
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