Morphological and Molecular Characterization of Diplogasteroides sp., a Cryptic Population of the Haslacheri Group (Diplogastridae), and Parasitorhabditis terebranus (Rhabditidae) from Korea
Diplogasteroides sp., a cryptic population of D. haslacheri, and Parasitorhabditis terebranus were reported from the frass of Monochamus alternatus galleries in dead Pinus thunbergii for the first time in Korea. Females and males are morphologically characterized and their linked DNA barcodes (18S-r...
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| Format: | Article |
| Language: | English |
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Sciendo
2023-05-01
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| Series: | Journal of Nematology |
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| Online Access: | https://doi.org/10.2478/jofnem-2023-0017 |
| _version_ | 1797219112852652032 |
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| author | Mwamula Abraham Okki Lee Sang Myeong Jung Young Hak Lee Ho-wook Kim Yi Seul Kim Young Ho Lee Dong Woon |
| author_facet | Mwamula Abraham Okki Lee Sang Myeong Jung Young Hak Lee Ho-wook Kim Yi Seul Kim Young Ho Lee Dong Woon |
| author_sort | Mwamula Abraham Okki |
| collection | DOAJ |
| description | Diplogasteroides sp., a cryptic population of D. haslacheri, and Parasitorhabditis terebranus were reported from the frass of Monochamus alternatus galleries in dead Pinus thunbergii for the first time in Korea. Females and males are morphologically characterized and their linked DNA barcodes (18S-rRNA, 28S-rRNA, ITS-rRNA and COI) supplied. Females and males of the two species from Korea conform to the original species descriptions from Europe and the USA, with variations in a few details in morphometrics. Specifically, Diplogasteroides sp. is morphologically very similar to D. haslacheri. However, it cannot be designated as D. haslacheri due to the existence of cryptic species complex within the haslacheri group (D. haslacheri, D. asiaticus, D. nix, D. andrassyi, and D. carinthiacus), a condition requiring hybridization studies to test species identity within the group. Based on analysis of COI sequences, differences among these cryptic species are evident. Thus, in addition to hybridization tests, the COI might be a powerful DNA barcoding marker for the precise identification of these cryptic species within the genus. Additionally, this is the first molecular characterization of P. terebranus, and the species is herein recorded for the first time outside its type locality. |
| first_indexed | 2024-03-08T10:02:38Z |
| format | Article |
| id | doaj.art-54d54a16028e41468902d06b1fc41030 |
| institution | Directory Open Access Journal |
| issn | 2640-396X |
| language | English |
| last_indexed | 2024-04-24T12:28:28Z |
| publishDate | 2023-05-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Nematology |
| spelling | doaj.art-54d54a16028e41468902d06b1fc410302024-04-08T07:37:21ZengSciendoJournal of Nematology2640-396X2023-05-01551495910.2478/jofnem-2023-0017Morphological and Molecular Characterization of Diplogasteroides sp., a Cryptic Population of the Haslacheri Group (Diplogastridae), and Parasitorhabditis terebranus (Rhabditidae) from KoreaMwamula Abraham Okki0Lee Sang Myeong1Jung Young Hak2Lee Ho-wook3Kim Yi Seul4Kim Young Ho5Lee Dong Woon61Department of Entomology, Kyungpook National University, Sangju, 37224, Republic of Korea3SM Biovision Co., Jinju, 52849, Republic of Korea3SM Biovision Co., Jinju, 52849, Republic of Korea4Department of Ecological Science, Kyungpook National University, Sangju, 37224, Republic of Korea1Department of Entomology, Kyungpook National University, Sangju, 37224, Republic of Korea1Department of Entomology, Kyungpook National University, Sangju, 37224, Republic of Korea1Department of Entomology, Kyungpook National University, Sangju, 37224, Republic of KoreaDiplogasteroides sp., a cryptic population of D. haslacheri, and Parasitorhabditis terebranus were reported from the frass of Monochamus alternatus galleries in dead Pinus thunbergii for the first time in Korea. Females and males are morphologically characterized and their linked DNA barcodes (18S-rRNA, 28S-rRNA, ITS-rRNA and COI) supplied. Females and males of the two species from Korea conform to the original species descriptions from Europe and the USA, with variations in a few details in morphometrics. Specifically, Diplogasteroides sp. is morphologically very similar to D. haslacheri. However, it cannot be designated as D. haslacheri due to the existence of cryptic species complex within the haslacheri group (D. haslacheri, D. asiaticus, D. nix, D. andrassyi, and D. carinthiacus), a condition requiring hybridization studies to test species identity within the group. Based on analysis of COI sequences, differences among these cryptic species are evident. Thus, in addition to hybridization tests, the COI might be a powerful DNA barcoding marker for the precise identification of these cryptic species within the genus. Additionally, this is the first molecular characterization of P. terebranus, and the species is herein recorded for the first time outside its type locality.https://doi.org/10.2478/jofnem-2023-0017diplogasteroidesmorphologyparasitorhabditis terebranustaxonomy |
| spellingShingle | Mwamula Abraham Okki Lee Sang Myeong Jung Young Hak Lee Ho-wook Kim Yi Seul Kim Young Ho Lee Dong Woon Morphological and Molecular Characterization of Diplogasteroides sp., a Cryptic Population of the Haslacheri Group (Diplogastridae), and Parasitorhabditis terebranus (Rhabditidae) from Korea Journal of Nematology diplogasteroides morphology parasitorhabditis terebranus taxonomy |
| title | Morphological and Molecular Characterization of Diplogasteroides sp., a Cryptic Population of the Haslacheri Group (Diplogastridae), and Parasitorhabditis terebranus (Rhabditidae) from Korea |
| title_full | Morphological and Molecular Characterization of Diplogasteroides sp., a Cryptic Population of the Haslacheri Group (Diplogastridae), and Parasitorhabditis terebranus (Rhabditidae) from Korea |
| title_fullStr | Morphological and Molecular Characterization of Diplogasteroides sp., a Cryptic Population of the Haslacheri Group (Diplogastridae), and Parasitorhabditis terebranus (Rhabditidae) from Korea |
| title_full_unstemmed | Morphological and Molecular Characterization of Diplogasteroides sp., a Cryptic Population of the Haslacheri Group (Diplogastridae), and Parasitorhabditis terebranus (Rhabditidae) from Korea |
| title_short | Morphological and Molecular Characterization of Diplogasteroides sp., a Cryptic Population of the Haslacheri Group (Diplogastridae), and Parasitorhabditis terebranus (Rhabditidae) from Korea |
| title_sort | morphological and molecular characterization of diplogasteroides sp a cryptic population of the haslacheri group diplogastridae and parasitorhabditis terebranus rhabditidae from korea |
| topic | diplogasteroides morphology parasitorhabditis terebranus taxonomy |
| url | https://doi.org/10.2478/jofnem-2023-0017 |
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