Early-life environmental variation affects intestinal microbiota and immune development in new-born piglets.
<h4>Background</h4>Early-life environmental variation affects gut microbial colonization and immune competence development; however, the timing and additional specifics of these processes are unknown. The impact of early-life environmental variations, as experienced under real life circu...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24941112/?tool=EBI |
| _version_ | 1819240865282916352 |
|---|---|
| author | Dirkjan Schokker Jing Zhang Ling-Li Zhang Stéphanie A Vastenhouw Hans G H J Heilig Hauke Smidt Johanna M J Rebel Mari A Smits |
| author_facet | Dirkjan Schokker Jing Zhang Ling-Li Zhang Stéphanie A Vastenhouw Hans G H J Heilig Hauke Smidt Johanna M J Rebel Mari A Smits |
| author_sort | Dirkjan Schokker |
| collection | DOAJ |
| description | <h4>Background</h4>Early-life environmental variation affects gut microbial colonization and immune competence development; however, the timing and additional specifics of these processes are unknown. The impact of early-life environmental variations, as experienced under real life circumstances, on gut microbial colonization and immune development has not been studied extensively so far. We designed a study to investigate environmental variation, experienced early after birth, to gut microbial colonization and intestinal immune development.<h4>Methodology/principal findings</h4>To investigate effects of early-life environmental changes, the piglets of 16 piglet litters were divided into 3 groups per litter and experimentally treated on day 4 after birth. During the course of the experiment, the piglets were kept with their mother sow. Group 1 was not treated, group 2 was treated with an antibiotic, and group 3 was treated with an antibiotic and simultaneously exposed to several routine, but stressful management procedures, including docking, clipping and weighing. Thereafter, treatment effects were measured at day 8 after birth in 16 piglets per treatment group by community-scale analysis of gut microbiota and genome-wide intestinal transcriptome profiling. We observed that the applied antibiotic treatment affected the composition and diversity of gut microbiota and reduced the expression of a large number of immune-related processes. The effect of management procedures on top of the use of an antibiotic was limited.<h4>Conclusions/significance</h4>We provide direct evidence that different early-life conditions, specifically focusing on antibiotic treatment and exposure to stress, affect gut microbial colonization and intestinal immune development. This reinforces the notion that the early phase of life is critical for intestinal immune development, also under regular production circumstances. |
| first_indexed | 2024-12-23T14:14:48Z |
| format | Article |
| id | doaj.art-54d67d8313aa485d85f8b4f15fad14e2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T14:14:48Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-54d67d8313aa485d85f8b4f15fad14e22022-12-21T17:43:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0196e10004010.1371/journal.pone.0100040Early-life environmental variation affects intestinal microbiota and immune development in new-born piglets.Dirkjan SchokkerJing ZhangLing-Li ZhangStéphanie A VastenhouwHans G H J HeiligHauke SmidtJohanna M J RebelMari A Smits<h4>Background</h4>Early-life environmental variation affects gut microbial colonization and immune competence development; however, the timing and additional specifics of these processes are unknown. The impact of early-life environmental variations, as experienced under real life circumstances, on gut microbial colonization and immune development has not been studied extensively so far. We designed a study to investigate environmental variation, experienced early after birth, to gut microbial colonization and intestinal immune development.<h4>Methodology/principal findings</h4>To investigate effects of early-life environmental changes, the piglets of 16 piglet litters were divided into 3 groups per litter and experimentally treated on day 4 after birth. During the course of the experiment, the piglets were kept with their mother sow. Group 1 was not treated, group 2 was treated with an antibiotic, and group 3 was treated with an antibiotic and simultaneously exposed to several routine, but stressful management procedures, including docking, clipping and weighing. Thereafter, treatment effects were measured at day 8 after birth in 16 piglets per treatment group by community-scale analysis of gut microbiota and genome-wide intestinal transcriptome profiling. We observed that the applied antibiotic treatment affected the composition and diversity of gut microbiota and reduced the expression of a large number of immune-related processes. The effect of management procedures on top of the use of an antibiotic was limited.<h4>Conclusions/significance</h4>We provide direct evidence that different early-life conditions, specifically focusing on antibiotic treatment and exposure to stress, affect gut microbial colonization and intestinal immune development. This reinforces the notion that the early phase of life is critical for intestinal immune development, also under regular production circumstances.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24941112/?tool=EBI |
| spellingShingle | Dirkjan Schokker Jing Zhang Ling-Li Zhang Stéphanie A Vastenhouw Hans G H J Heilig Hauke Smidt Johanna M J Rebel Mari A Smits Early-life environmental variation affects intestinal microbiota and immune development in new-born piglets. PLoS ONE |
| title | Early-life environmental variation affects intestinal microbiota and immune development in new-born piglets. |
| title_full | Early-life environmental variation affects intestinal microbiota and immune development in new-born piglets. |
| title_fullStr | Early-life environmental variation affects intestinal microbiota and immune development in new-born piglets. |
| title_full_unstemmed | Early-life environmental variation affects intestinal microbiota and immune development in new-born piglets. |
| title_short | Early-life environmental variation affects intestinal microbiota and immune development in new-born piglets. |
| title_sort | early life environmental variation affects intestinal microbiota and immune development in new born piglets |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24941112/?tool=EBI |
| work_keys_str_mv | AT dirkjanschokker earlylifeenvironmentalvariationaffectsintestinalmicrobiotaandimmunedevelopmentinnewbornpiglets AT jingzhang earlylifeenvironmentalvariationaffectsintestinalmicrobiotaandimmunedevelopmentinnewbornpiglets AT linglizhang earlylifeenvironmentalvariationaffectsintestinalmicrobiotaandimmunedevelopmentinnewbornpiglets AT stephanieavastenhouw earlylifeenvironmentalvariationaffectsintestinalmicrobiotaandimmunedevelopmentinnewbornpiglets AT hansghjheilig earlylifeenvironmentalvariationaffectsintestinalmicrobiotaandimmunedevelopmentinnewbornpiglets AT haukesmidt earlylifeenvironmentalvariationaffectsintestinalmicrobiotaandimmunedevelopmentinnewbornpiglets AT johannamjrebel earlylifeenvironmentalvariationaffectsintestinalmicrobiotaandimmunedevelopmentinnewbornpiglets AT mariasmits earlylifeenvironmentalvariationaffectsintestinalmicrobiotaandimmunedevelopmentinnewbornpiglets |