Analysis of genetic diversity in Pa Co pine (Pinus kwangtungensis Chun ex Tsiang) using RAPD and ISSR markers
Pinus kwangtungensis (Pa Co pine) is one of three five-needle pine species in Vietnam, found on the slopes of limestone mountains at altitudes between 1200 and 1500 m. Global warming and long-term deforestation threaten the existence of the species in nature. The genetic diversity of plant populati...
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| Format: | Article |
| Language: | English |
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Vietnam Ministry of Science and Technology
2022-03-01
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| Series: | Vietnam Journal of Science, Technology and Engineering |
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| Online Access: | https://vietnamscience.vjst.vn/index.php/vjste/article/view/143 |
| _version_ | 1828047893671444480 |
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| author | Mai Thi Phuong Thuy Tran Thi Thu Ha Tran Ho Quang |
| author_facet | Mai Thi Phuong Thuy Tran Thi Thu Ha Tran Ho Quang |
| author_sort | Mai Thi Phuong Thuy |
| collection | DOAJ |
| description |
Pinus kwangtungensis (Pa Co pine) is one of three five-needle pine species in Vietnam, found on the slopes of limestone mountains at altitudes between 1200 and 1500 m. Global warming and long-term deforestation threaten the existence of the species in nature. The genetic diversity of plant populations provides a background for future conservation and improvement programmes. However, the genetic diversity of Pa Co pine is unknown. This study aimed to use inter-simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) genetic markers to evaluate the genetic-diversity parameters of P. kwangtungensis to understand the genetic effects of small and fragmented populations, as well as provide the genetic background for its conservation. Total genomic DNA was extracted from fresh needles of 40 trees in four different areas and amplified with 15 RAPD and 16 ISSR markers. Results indicated that the genetic diversity index (h) of P. kwangtungensis was 0.2530 with RAPD and 0.223 with ISSR. High genetic variation was found within populations (72% with RAPD and 87% with ISSR). Principal coordinates analysis based on RAPD analysis revealed that the presence of three groups was in accordance, whereas no clear cluster was formed according to ISSR analysis. The results from this study enhance the understanding of the genetic effects of small and fragmented populations of native species that are rare, vulnerable, and require conservation.
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| first_indexed | 2024-04-10T18:50:17Z |
| format | Article |
| id | doaj.art-54d9154f025a44b1aa2a17e759703e1a |
| institution | Directory Open Access Journal |
| issn | 2525-2461 2615-9937 |
| language | English |
| last_indexed | 2024-04-10T18:50:17Z |
| publishDate | 2022-03-01 |
| publisher | Vietnam Ministry of Science and Technology |
| record_format | Article |
| series | Vietnam Journal of Science, Technology and Engineering |
| spelling | doaj.art-54d9154f025a44b1aa2a17e759703e1a2023-02-01T08:16:23ZengVietnam Ministry of Science and TechnologyVietnam Journal of Science, Technology and Engineering2525-24612615-99372022-03-0162110.31276/VJSTE.62(1).62-68Analysis of genetic diversity in Pa Co pine (Pinus kwangtungensis Chun ex Tsiang) using RAPD and ISSR markersMai Thi Phuong Thuy0Tran Thi Thu Ha1Tran Ho Quang2Institute of Forest Tree Improvement and Biotechnology, Vietnamese Academy of Forest SciencesInstitute of Forest Tree Improvement and Biotechnology, Vietnamese Academy of Forest SciencesInstitute of Biotechnology, Vietnam Academy of Science and Technology Pinus kwangtungensis (Pa Co pine) is one of three five-needle pine species in Vietnam, found on the slopes of limestone mountains at altitudes between 1200 and 1500 m. Global warming and long-term deforestation threaten the existence of the species in nature. The genetic diversity of plant populations provides a background for future conservation and improvement programmes. However, the genetic diversity of Pa Co pine is unknown. This study aimed to use inter-simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) genetic markers to evaluate the genetic-diversity parameters of P. kwangtungensis to understand the genetic effects of small and fragmented populations, as well as provide the genetic background for its conservation. Total genomic DNA was extracted from fresh needles of 40 trees in four different areas and amplified with 15 RAPD and 16 ISSR markers. Results indicated that the genetic diversity index (h) of P. kwangtungensis was 0.2530 with RAPD and 0.223 with ISSR. High genetic variation was found within populations (72% with RAPD and 87% with ISSR). Principal coordinates analysis based on RAPD analysis revealed that the presence of three groups was in accordance, whereas no clear cluster was formed according to ISSR analysis. The results from this study enhance the understanding of the genetic effects of small and fragmented populations of native species that are rare, vulnerable, and require conservation. https://vietnamscience.vjst.vn/index.php/vjste/article/view/143conservationgenetic diversityISSRPinus kwangtungensisRAPD |
| spellingShingle | Mai Thi Phuong Thuy Tran Thi Thu Ha Tran Ho Quang Analysis of genetic diversity in Pa Co pine (Pinus kwangtungensis Chun ex Tsiang) using RAPD and ISSR markers Vietnam Journal of Science, Technology and Engineering conservation genetic diversity ISSR Pinus kwangtungensis RAPD |
| title | Analysis of genetic diversity in Pa Co pine (Pinus kwangtungensis Chun ex Tsiang) using RAPD and ISSR markers |
| title_full | Analysis of genetic diversity in Pa Co pine (Pinus kwangtungensis Chun ex Tsiang) using RAPD and ISSR markers |
| title_fullStr | Analysis of genetic diversity in Pa Co pine (Pinus kwangtungensis Chun ex Tsiang) using RAPD and ISSR markers |
| title_full_unstemmed | Analysis of genetic diversity in Pa Co pine (Pinus kwangtungensis Chun ex Tsiang) using RAPD and ISSR markers |
| title_short | Analysis of genetic diversity in Pa Co pine (Pinus kwangtungensis Chun ex Tsiang) using RAPD and ISSR markers |
| title_sort | analysis of genetic diversity in pa co pine pinus kwangtungensis chun ex tsiang using rapd and issr markers |
| topic | conservation genetic diversity ISSR Pinus kwangtungensis RAPD |
| url | https://vietnamscience.vjst.vn/index.php/vjste/article/view/143 |
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