Antihyperlipidemic effects of apple peel extract in high-fat diet-induced hyperlipidemic rats
Hyperlipidemia is generally managed with statin-based drugs. Simvastatin serves as a 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) inhibitor, with prolonged use proven to cause side effects. In the present study, antihyperlipidemic material is tested for its effect in lowering lipid in anim...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2020-01-01
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| Series: | Journal of Advanced Pharmaceutical Technology & Research |
| Subjects: | |
| Online Access: | http://www.japtr.org/article.asp?issn=2231-4040;year=2020;volume=11;issue=3;spage=128;epage=133;aulast=Susilowati |
| _version_ | 1819117354456449024 |
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| author | Retno Susilowati Jauharotul Jannah Zahrotul Maghfuroh Meike Tiya Kusuma |
| author_facet | Retno Susilowati Jauharotul Jannah Zahrotul Maghfuroh Meike Tiya Kusuma |
| author_sort | Retno Susilowati |
| collection | DOAJ |
| description | Hyperlipidemia is generally managed with statin-based drugs. Simvastatin serves as a 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) inhibitor, with prolonged use proven to cause side effects. In the present study, antihyperlipidemic material is tested for its effect in lowering lipid in animals and its proven ability to bind to HMGR. Hyperlipidemia rats were divided into four groups, with different doses of 0, 57, and 114 mg/kg BW of apple peel extract (APE) and simvastatin (3.6 mg/kg BW). The total cholesterol (TC), total triglyceride (TG), low-density lipoprotein cholesterol (LDLc), and high-density lipoprotein cholesterol (HDLc) serum were measured. In silico inhibition test of HMGR activity was conducted by molecular docking using PyRx software. This process places HMGR as a receptor and active compound of apple peels as a ligand. APE treatment with a dose of 114 mg/kg BW could significantly reduce LDLc and increase serum HDLc levels. Docking tests confirmed that quercetin, chlorogenic acid, epicatechin, and catechins depicted HMGR inhibition. Quercetin could bind to HMGR at a similar location to amino acid residues as simvastatin. These material extracts have inhibited cholesterol synthesis through a stronger HMGR inhibition than simvastatin. |
| first_indexed | 2024-12-22T05:31:39Z |
| format | Article |
| id | doaj.art-54d9b3454961491ba8ccb8997de1ad0b |
| institution | Directory Open Access Journal |
| issn | 2231-4040 0976-2094 |
| language | English |
| last_indexed | 2024-12-22T05:31:39Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Advanced Pharmaceutical Technology & Research |
| spelling | doaj.art-54d9b3454961491ba8ccb8997de1ad0b2022-12-21T18:37:26ZengWolters Kluwer Medknow PublicationsJournal of Advanced Pharmaceutical Technology & Research2231-40400976-20942020-01-0111312813310.4103/japtr.JAPTR_28_20Antihyperlipidemic effects of apple peel extract in high-fat diet-induced hyperlipidemic ratsRetno SusilowatiJauharotul JannahZahrotul MaghfurohMeike Tiya KusumaHyperlipidemia is generally managed with statin-based drugs. Simvastatin serves as a 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) inhibitor, with prolonged use proven to cause side effects. In the present study, antihyperlipidemic material is tested for its effect in lowering lipid in animals and its proven ability to bind to HMGR. Hyperlipidemia rats were divided into four groups, with different doses of 0, 57, and 114 mg/kg BW of apple peel extract (APE) and simvastatin (3.6 mg/kg BW). The total cholesterol (TC), total triglyceride (TG), low-density lipoprotein cholesterol (LDLc), and high-density lipoprotein cholesterol (HDLc) serum were measured. In silico inhibition test of HMGR activity was conducted by molecular docking using PyRx software. This process places HMGR as a receptor and active compound of apple peels as a ligand. APE treatment with a dose of 114 mg/kg BW could significantly reduce LDLc and increase serum HDLc levels. Docking tests confirmed that quercetin, chlorogenic acid, epicatechin, and catechins depicted HMGR inhibition. Quercetin could bind to HMGR at a similar location to amino acid residues as simvastatin. These material extracts have inhibited cholesterol synthesis through a stronger HMGR inhibition than simvastatin.http://www.japtr.org/article.asp?issn=2231-4040;year=2020;volume=11;issue=3;spage=128;epage=133;aulast=Susilowatiantihyperlipidemicapple peelshigh-density lipoprotein cholesterolhydroxy-3-methylglutaryl coenzyme a reductaselow-density lipoprotein cholesteroltriglyceride |
| spellingShingle | Retno Susilowati Jauharotul Jannah Zahrotul Maghfuroh Meike Tiya Kusuma Antihyperlipidemic effects of apple peel extract in high-fat diet-induced hyperlipidemic rats Journal of Advanced Pharmaceutical Technology & Research antihyperlipidemic apple peels high-density lipoprotein cholesterol hydroxy-3-methylglutaryl coenzyme a reductase low-density lipoprotein cholesterol triglyceride |
| title | Antihyperlipidemic effects of apple peel extract in high-fat diet-induced hyperlipidemic rats |
| title_full | Antihyperlipidemic effects of apple peel extract in high-fat diet-induced hyperlipidemic rats |
| title_fullStr | Antihyperlipidemic effects of apple peel extract in high-fat diet-induced hyperlipidemic rats |
| title_full_unstemmed | Antihyperlipidemic effects of apple peel extract in high-fat diet-induced hyperlipidemic rats |
| title_short | Antihyperlipidemic effects of apple peel extract in high-fat diet-induced hyperlipidemic rats |
| title_sort | antihyperlipidemic effects of apple peel extract in high fat diet induced hyperlipidemic rats |
| topic | antihyperlipidemic apple peels high-density lipoprotein cholesterol hydroxy-3-methylglutaryl coenzyme a reductase low-density lipoprotein cholesterol triglyceride |
| url | http://www.japtr.org/article.asp?issn=2231-4040;year=2020;volume=11;issue=3;spage=128;epage=133;aulast=Susilowati |
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