Platinum Nanoparticles Suppress Osteoclastogenesis Through Scavenging of Reactive Oxygen Species Produced in RAW264.7 Cells
Abstract.: Recent research has shown that platinum nanoparticles (nano-Pt) efficiently quench reactive oxygen species (ROS) as a reducing catalyst. ROS have been suggested to regulate receptor activator of NF-κB ligand (RANKL)-stimulated osteoclast differentiation. In the present study, we examined...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2011-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319306115 |
| _version_ | 1818030710965927936 |
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| author | Mayumi Nomura Yoshitaka Yoshimura Takashi Kikuiri Tomokazu Hasegawa Yumi Taniguchi Yoshiaki Deyama Ken-ichi Koshiro Hidehiko Sano Kuniaki Suzuki Nobuo Inoue |
| author_facet | Mayumi Nomura Yoshitaka Yoshimura Takashi Kikuiri Tomokazu Hasegawa Yumi Taniguchi Yoshiaki Deyama Ken-ichi Koshiro Hidehiko Sano Kuniaki Suzuki Nobuo Inoue |
| author_sort | Mayumi Nomura |
| collection | DOAJ |
| description | Abstract.: Recent research has shown that platinum nanoparticles (nano-Pt) efficiently quench reactive oxygen species (ROS) as a reducing catalyst. ROS have been suggested to regulate receptor activator of NF-κB ligand (RANKL)-stimulated osteoclast differentiation. In the present study, we examined the direct effects of platinum nano-Pt on RANKL-induced osteoclast differentiation of murine pre-osteoclastic RAW 264.7 cells. The effect of the nano-Pt on the number of osteoclasts was measured and their effect on the mRNA expression for osteoclast differentiation was assayed using real-time PCR. Nano-Pt appeared to have a ROS-scavenging activity. Nano-Pt decreased the number of osteoclasts (2+ nuclei) and large osteoclasts (8+ nuclei) in a dose-dependent manner without affecting cell viability. In addition, this agent significantly blocked RANKL-induced mRNA expression of osteoclastic differentiation genes such as c-fms, NFATc1, NFATc2, and DC-STAMP as well as that of osteoclast-specific marker genes including MMP-9, Cath-K, CLC7, ATP6i, CTR, and TRAP. Although nano-Pt attenuated expression of the ROS-producing NOX-family oxidases, Nox1 and Nox4, they up-regulated expression of Nox2, the major Nox enzyme in macrophages. These findings suggest that the nano-Pt inhibit RANKL-stimulated osteoclast differentiation via their ROS scavenging property. The use of nano-Pt as scavengers of ROS that is generated by RANKL may be a novel and innovative therapy for bone diseases. Keywords:: platinum nanoparticle, osteoclast, reactive oxygen species (ROS), NOX, RAW cell |
| first_indexed | 2024-12-10T05:39:55Z |
| format | Article |
| id | doaj.art-54dc497ecc5a418a9b64da455f60b6e7 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-10T05:39:55Z |
| publishDate | 2011-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-54dc497ecc5a418a9b64da455f60b6e72022-12-22T02:00:18ZengElsevierJournal of Pharmacological Sciences1347-86132011-01-011174243252Platinum Nanoparticles Suppress Osteoclastogenesis Through Scavenging of Reactive Oxygen Species Produced in RAW264.7 CellsMayumi Nomura0Yoshitaka Yoshimura1Takashi Kikuiri2Tomokazu Hasegawa3Yumi Taniguchi4Yoshiaki Deyama5Ken-ichi Koshiro6Hidehiko Sano7Kuniaki Suzuki8Nobuo Inoue9Department of Gerodontology, Kita-ku, Sapporo 060-8586, Japan; Department of Molecular Cell Pharmacology, Kita-ku, Sapporo 060-8586, JapanDepartment of Molecular Cell Pharmacology, Kita-ku, Sapporo 060-8586, Japan; Corresponding author. yoshi@den.hokudai.ac.jpDepartment of Pediatric Dentistry, and Kita-ku, Sapporo 060-8586, JapanDepartment of Pediatric Dentistry, Faculty of Dentistry, Tokushima University, 3-18-15, Kuramoto-cho, Tokushima 770-8504, JapanDepartment of Pediatric Dentistry, and Kita-ku, Sapporo 060-8586, JapanDepartment of Molecular Cell Pharmacology, Kita-ku, Sapporo 060-8586, JapanDepartment of Restorative Dentistry, Hokkaido University Graduate School of Dental Medicine, Kita-ku, Sapporo 060-8586, JapanDepartment of Restorative Dentistry, Hokkaido University Graduate School of Dental Medicine, Kita-ku, Sapporo 060-8586, JapanDepartment of Molecular Cell Pharmacology, Kita-ku, Sapporo 060-8586, JapanDepartment of Gerodontology, Kita-ku, Sapporo 060-8586, JapanAbstract.: Recent research has shown that platinum nanoparticles (nano-Pt) efficiently quench reactive oxygen species (ROS) as a reducing catalyst. ROS have been suggested to regulate receptor activator of NF-κB ligand (RANKL)-stimulated osteoclast differentiation. In the present study, we examined the direct effects of platinum nano-Pt on RANKL-induced osteoclast differentiation of murine pre-osteoclastic RAW 264.7 cells. The effect of the nano-Pt on the number of osteoclasts was measured and their effect on the mRNA expression for osteoclast differentiation was assayed using real-time PCR. Nano-Pt appeared to have a ROS-scavenging activity. Nano-Pt decreased the number of osteoclasts (2+ nuclei) and large osteoclasts (8+ nuclei) in a dose-dependent manner without affecting cell viability. In addition, this agent significantly blocked RANKL-induced mRNA expression of osteoclastic differentiation genes such as c-fms, NFATc1, NFATc2, and DC-STAMP as well as that of osteoclast-specific marker genes including MMP-9, Cath-K, CLC7, ATP6i, CTR, and TRAP. Although nano-Pt attenuated expression of the ROS-producing NOX-family oxidases, Nox1 and Nox4, they up-regulated expression of Nox2, the major Nox enzyme in macrophages. These findings suggest that the nano-Pt inhibit RANKL-stimulated osteoclast differentiation via their ROS scavenging property. The use of nano-Pt as scavengers of ROS that is generated by RANKL may be a novel and innovative therapy for bone diseases. Keywords:: platinum nanoparticle, osteoclast, reactive oxygen species (ROS), NOX, RAW cellhttp://www.sciencedirect.com/science/article/pii/S1347861319306115 |
| spellingShingle | Mayumi Nomura Yoshitaka Yoshimura Takashi Kikuiri Tomokazu Hasegawa Yumi Taniguchi Yoshiaki Deyama Ken-ichi Koshiro Hidehiko Sano Kuniaki Suzuki Nobuo Inoue Platinum Nanoparticles Suppress Osteoclastogenesis Through Scavenging of Reactive Oxygen Species Produced in RAW264.7 Cells Journal of Pharmacological Sciences |
| title | Platinum Nanoparticles Suppress Osteoclastogenesis Through Scavenging of Reactive Oxygen Species Produced in RAW264.7 Cells |
| title_full | Platinum Nanoparticles Suppress Osteoclastogenesis Through Scavenging of Reactive Oxygen Species Produced in RAW264.7 Cells |
| title_fullStr | Platinum Nanoparticles Suppress Osteoclastogenesis Through Scavenging of Reactive Oxygen Species Produced in RAW264.7 Cells |
| title_full_unstemmed | Platinum Nanoparticles Suppress Osteoclastogenesis Through Scavenging of Reactive Oxygen Species Produced in RAW264.7 Cells |
| title_short | Platinum Nanoparticles Suppress Osteoclastogenesis Through Scavenging of Reactive Oxygen Species Produced in RAW264.7 Cells |
| title_sort | platinum nanoparticles suppress osteoclastogenesis through scavenging of reactive oxygen species produced in raw264 7 cells |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319306115 |
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