A label-free thrombin photoelectrochemical aptasensor based on structure-switching in G-quadruplexes
In this work, an Au/GaN photoelectrode was prepared by sputtering a 30.0-nm–thick gold film on an n-type gallium nitride (GaN) surface. Guanine (G) was used as a hole trap in photoinduced charge separation due to its lowest oxidation potential and because it could be easily oxidized among the five c...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Biosensors and Bioelectronics: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590137022000541 |
| _version_ | 1811188798267064320 |
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| author | Xiujun Rong Huilan Zhang Weicheng Cai Fang Luo Cuiying Lin Bin Qiu Zhenyu Lin Jian Wang Zhou Jiang Hui Huang |
| author_facet | Xiujun Rong Huilan Zhang Weicheng Cai Fang Luo Cuiying Lin Bin Qiu Zhenyu Lin Jian Wang Zhou Jiang Hui Huang |
| author_sort | Xiujun Rong |
| collection | DOAJ |
| description | In this work, an Au/GaN photoelectrode was prepared by sputtering a 30.0-nm–thick gold film on an n-type gallium nitride (GaN) surface. Guanine (G) was used as a hole trap in photoinduced charge separation due to its lowest oxidation potential and because it could be easily oxidized among the five common nucleobases. Guanosine-rich DNA sequences can form stable secondary structures known as G-quadruplexes. The efficiency of hole transport was significantly improved after G-quadruplexes formation. The thrombin aptamer rich in guanine could be modified on the electrode surface through Au–S bonds, forming stable G-quadruplexes in the presence of target thrombin. Due to the presence of the G-quadruplexes, the electron-hole recombination rate of the system decreased and the photocurrent increased. Under optimized conditions, high specificity, selectivity, and linearity could be achieved in the concentration range of 0.100–10.0 nm, and the limit of detection was approximately 20.0 pM. Thus, the detection results of human serum thrombin were satisfactory. |
| first_indexed | 2024-04-11T14:24:20Z |
| format | Article |
| id | doaj.art-54de914cae804cdfbaf3de4b782ce147 |
| institution | Directory Open Access Journal |
| issn | 2590-1370 |
| language | English |
| last_indexed | 2024-04-11T14:24:20Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj.art-54de914cae804cdfbaf3de4b782ce1472022-12-22T04:18:55ZengElsevierBiosensors and Bioelectronics: X2590-13702022-09-0111100159A label-free thrombin photoelectrochemical aptasensor based on structure-switching in G-quadruplexesXiujun Rong0Huilan Zhang1Weicheng Cai2Fang Luo3Cuiying Lin4Bin Qiu5Zhenyu Lin6Jian Wang7Zhou Jiang8Hui Huang9Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, PR ChinaMinistry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, PR ChinaKey Lab of Liaoning IC Technology, School of Biomedical Engineer, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Dalian, 116024, ChinaCollege of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350116, ChinaCollege of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, ChinaMinistry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, PR ChinaMinistry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, PR ChinaMinistry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, PR China; Corresponding author. 2 Xue Yuan Road, University Town Fuzhou, Fujian, PR China.College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, China; Corresponding author. 2 Xue Yuan Road, University Town Fuzhou, Fujian, PR China.Key Lab of Liaoning IC Technology, School of Biomedical Engineer, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Dalian, 116024, China; Corresponding author. Dalian University of Technology, High-tech District, Dalian, Liaoning, PR China.In this work, an Au/GaN photoelectrode was prepared by sputtering a 30.0-nm–thick gold film on an n-type gallium nitride (GaN) surface. Guanine (G) was used as a hole trap in photoinduced charge separation due to its lowest oxidation potential and because it could be easily oxidized among the five common nucleobases. Guanosine-rich DNA sequences can form stable secondary structures known as G-quadruplexes. The efficiency of hole transport was significantly improved after G-quadruplexes formation. The thrombin aptamer rich in guanine could be modified on the electrode surface through Au–S bonds, forming stable G-quadruplexes in the presence of target thrombin. Due to the presence of the G-quadruplexes, the electron-hole recombination rate of the system decreased and the photocurrent increased. Under optimized conditions, high specificity, selectivity, and linearity could be achieved in the concentration range of 0.100–10.0 nm, and the limit of detection was approximately 20.0 pM. Thus, the detection results of human serum thrombin were satisfactory.http://www.sciencedirect.com/science/article/pii/S2590137022000541PhotoelectrochemicalG-quadruplexThrombin |
| spellingShingle | Xiujun Rong Huilan Zhang Weicheng Cai Fang Luo Cuiying Lin Bin Qiu Zhenyu Lin Jian Wang Zhou Jiang Hui Huang A label-free thrombin photoelectrochemical aptasensor based on structure-switching in G-quadruplexes Biosensors and Bioelectronics: X Photoelectrochemical G-quadruplex Thrombin |
| title | A label-free thrombin photoelectrochemical aptasensor based on structure-switching in G-quadruplexes |
| title_full | A label-free thrombin photoelectrochemical aptasensor based on structure-switching in G-quadruplexes |
| title_fullStr | A label-free thrombin photoelectrochemical aptasensor based on structure-switching in G-quadruplexes |
| title_full_unstemmed | A label-free thrombin photoelectrochemical aptasensor based on structure-switching in G-quadruplexes |
| title_short | A label-free thrombin photoelectrochemical aptasensor based on structure-switching in G-quadruplexes |
| title_sort | label free thrombin photoelectrochemical aptasensor based on structure switching in g quadruplexes |
| topic | Photoelectrochemical G-quadruplex Thrombin |
| url | http://www.sciencedirect.com/science/article/pii/S2590137022000541 |
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