Chromosome evolution in Lophyohylini (Amphibia, Anura, Hylinae).
The hyline tribe Lophyohylini includes 87 species of treefrogs, of which cytogenetics aspects have been studied in less than 20% of them. In order to evaluate the evolution of some of its chromosome characters (NOR position, C-bands, and DAPI/CMA3 bands), we studied the karyotypes of 21 lophyohyline...
| Main Authors: | , , , , , , , , , , , , , , , |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0234331 |
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| author | Pablo Suárez Juan M Ferro Cleusa Y Nagamachi Dario E Cardozo Ailin Blasco-Zúñiga Jéssica B Silva Euvaldo Marciano-Jr Marco A Costa Victor G D Orrico Mirco Solé Igor J Roberto Miryan Rivera John E Wiley Julián Faivovich Diego Baldo Julio C Pieczarka |
| author_facet | Pablo Suárez Juan M Ferro Cleusa Y Nagamachi Dario E Cardozo Ailin Blasco-Zúñiga Jéssica B Silva Euvaldo Marciano-Jr Marco A Costa Victor G D Orrico Mirco Solé Igor J Roberto Miryan Rivera John E Wiley Julián Faivovich Diego Baldo Julio C Pieczarka |
| author_sort | Pablo Suárez |
| collection | DOAJ |
| description | The hyline tribe Lophyohylini includes 87 species of treefrogs, of which cytogenetics aspects have been studied in less than 20% of them. In order to evaluate the evolution of some of its chromosome characters (NOR position, C-bands, and DAPI/CMA3 bands), we studied the karyotypes of 21 lophyohylines, 16 of them for the first time, and analyzed them in a phylogenetic context. Most species showed similar karyotypes regarding chromosome number (2n = 24) and morphology (FN = 48), excepting Phyllodytes edelmoi and Osteocephalus buckleyi with 2n = 22 (FN = 44) and 2n = 28 (FN = 50), respectively. The NOR location was variable among species and provided valuable phylogenetic information. This marker was located in pair 11 in all species of Trachycephalus, Itapotihyla langsdorffii, and Nyctimantis arapapa, representing the plesiomorphic condition of Lophyohylini. Besides, other apomorphic states were recovered for the clades comprising N. rugiceps and N. siemersi (NOR in pair 5), and Dryaderces pearsoni, Osteocephalus, and Osteopilus (NOR in pair 9). Phyllodytes presented variation for NORs position; they were in pair 2 in P. edelmoi, pair 7 in P. melanomystax, and pair 8 in P. gyrinaethes and P. praeceptor. Polymorphisms in size, number, and activity of this marker were observed for N. siemersi, Osteocephalus fuscifacies, and some species of Trachycephalus. Remarkably, in N. siemersi NORs were detected on a single chromosome in the two specimens studied by this technique, raising the question of how this complex polymorphism is maintained. Interstitial telomeric sequences were found in P. edelmoi, P. melanomystax, and Osteocephalus buckleyi, and their presence seems to be not related to the chromosome reorganization events. Finally, some species showed spontaneous rearrangements, possibly as a consequence of an uncommon phenomenon in anuran cytogenetics: the presence of fragile sites or secondary constrictions not associated with NORs. We propose that this rare feature would have played an important role in the evolution of this group of frogs. From the evidence obtained in this and previous studies, we conclude that Lophyohylini presents a complex chromosome evolution. |
| first_indexed | 2024-12-13T21:26:51Z |
| format | Article |
| id | doaj.art-54defb1658804853812ef09f29a6f5e7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T21:26:51Z |
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| spelling | doaj.art-54defb1658804853812ef09f29a6f5e72022-12-21T23:30:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01156e023433110.1371/journal.pone.0234331Chromosome evolution in Lophyohylini (Amphibia, Anura, Hylinae).Pablo SuárezJuan M FerroCleusa Y NagamachiDario E CardozoAilin Blasco-ZúñigaJéssica B SilvaEuvaldo Marciano-JrMarco A CostaVictor G D OrricoMirco SoléIgor J RobertoMiryan RiveraJohn E WileyJulián FaivovichDiego BaldoJulio C PieczarkaThe hyline tribe Lophyohylini includes 87 species of treefrogs, of which cytogenetics aspects have been studied in less than 20% of them. In order to evaluate the evolution of some of its chromosome characters (NOR position, C-bands, and DAPI/CMA3 bands), we studied the karyotypes of 21 lophyohylines, 16 of them for the first time, and analyzed them in a phylogenetic context. Most species showed similar karyotypes regarding chromosome number (2n = 24) and morphology (FN = 48), excepting Phyllodytes edelmoi and Osteocephalus buckleyi with 2n = 22 (FN = 44) and 2n = 28 (FN = 50), respectively. The NOR location was variable among species and provided valuable phylogenetic information. This marker was located in pair 11 in all species of Trachycephalus, Itapotihyla langsdorffii, and Nyctimantis arapapa, representing the plesiomorphic condition of Lophyohylini. Besides, other apomorphic states were recovered for the clades comprising N. rugiceps and N. siemersi (NOR in pair 5), and Dryaderces pearsoni, Osteocephalus, and Osteopilus (NOR in pair 9). Phyllodytes presented variation for NORs position; they were in pair 2 in P. edelmoi, pair 7 in P. melanomystax, and pair 8 in P. gyrinaethes and P. praeceptor. Polymorphisms in size, number, and activity of this marker were observed for N. siemersi, Osteocephalus fuscifacies, and some species of Trachycephalus. Remarkably, in N. siemersi NORs were detected on a single chromosome in the two specimens studied by this technique, raising the question of how this complex polymorphism is maintained. Interstitial telomeric sequences were found in P. edelmoi, P. melanomystax, and Osteocephalus buckleyi, and their presence seems to be not related to the chromosome reorganization events. Finally, some species showed spontaneous rearrangements, possibly as a consequence of an uncommon phenomenon in anuran cytogenetics: the presence of fragile sites or secondary constrictions not associated with NORs. We propose that this rare feature would have played an important role in the evolution of this group of frogs. From the evidence obtained in this and previous studies, we conclude that Lophyohylini presents a complex chromosome evolution.https://doi.org/10.1371/journal.pone.0234331 |
| spellingShingle | Pablo Suárez Juan M Ferro Cleusa Y Nagamachi Dario E Cardozo Ailin Blasco-Zúñiga Jéssica B Silva Euvaldo Marciano-Jr Marco A Costa Victor G D Orrico Mirco Solé Igor J Roberto Miryan Rivera John E Wiley Julián Faivovich Diego Baldo Julio C Pieczarka Chromosome evolution in Lophyohylini (Amphibia, Anura, Hylinae). PLoS ONE |
| title | Chromosome evolution in Lophyohylini (Amphibia, Anura, Hylinae). |
| title_full | Chromosome evolution in Lophyohylini (Amphibia, Anura, Hylinae). |
| title_fullStr | Chromosome evolution in Lophyohylini (Amphibia, Anura, Hylinae). |
| title_full_unstemmed | Chromosome evolution in Lophyohylini (Amphibia, Anura, Hylinae). |
| title_short | Chromosome evolution in Lophyohylini (Amphibia, Anura, Hylinae). |
| title_sort | chromosome evolution in lophyohylini amphibia anura hylinae |
| url | https://doi.org/10.1371/journal.pone.0234331 |
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