Prognostic value of hypoxia-responsive gene expression profile in patients diagnosed with head and neck squamous cell carcinoma
Head and neck squamous cell carcinoma (HNSCC) is a disease associated with a severe mortality and high risk of distant metastasis and local recurrence. Currently, surgery and radiotherapy are the main treatment modes, however, therapeutic efficacy of radiotherapy is linked to tumor resistance. Hypox...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-01-01
|
| Series: | Translational Oncology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1936523323002279 |
| _version_ | 1797422632134508544 |
|---|---|
| author | Natasa Matic Lina Pettersson Felicia Sellebjerg Lina Lindberg Karin Roberg Emilia Wiechec |
| author_facet | Natasa Matic Lina Pettersson Felicia Sellebjerg Lina Lindberg Karin Roberg Emilia Wiechec |
| author_sort | Natasa Matic |
| collection | DOAJ |
| description | Head and neck squamous cell carcinoma (HNSCC) is a disease associated with a severe mortality and high risk of distant metastasis and local recurrence. Currently, surgery and radiotherapy are the main treatment modes, however, therapeutic efficacy of radiotherapy is linked to tumor resistance. Hypoxia has been shown to affect outcome of radiotherapy in HNSCC patients. The aim of this study was to verify the expression of the previously identified hypoxia-responsive genes (CA9, CASP14, LOX, GLUT3, SERPINE1, AREG, EREG, CCNB1 and KIF14) in HNSCC patient material as well as assess their prognostic potential. Tumor biopsies obtained before start of radiotherapy from 32 HNSCC patients classified as responders or non-responders were investigated in this study. The mRNA expression was quantified using RT-qPCR. The mRNA expression of CA9, SERPINE1 and KIF14 was significantly higher in the analyzed patient material compared with the non-cancerous oral tissue. Moreover, the KIF14 mRNA expression was significantly higher in the responder group compared to non-responders. Further studies demonstrated that knockdown of KIF14 reverses its radiosensitizing capability. Additionally, low expression of KIF14 mRNA correlated with significantly shorter OS (overall survival). In conclusion, our results suggest that KIF14 might be a useful prognostic and predictive marker in HNSCC. |
| first_indexed | 2024-03-09T07:35:08Z |
| format | Article |
| id | doaj.art-54e55084fb854c1e82eaa417f5b8c2cb |
| institution | Directory Open Access Journal |
| issn | 1936-5233 |
| language | English |
| last_indexed | 2024-03-09T07:35:08Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj.art-54e55084fb854c1e82eaa417f5b8c2cb2023-12-03T05:40:31ZengElsevierTranslational Oncology1936-52332024-01-0139101841Prognostic value of hypoxia-responsive gene expression profile in patients diagnosed with head and neck squamous cell carcinomaNatasa Matic0Lina Pettersson1Felicia Sellebjerg2Lina Lindberg3Karin Roberg4Emilia Wiechec5Department of Otorhinolaryngology in Linköping, Anaesthetics, Operations and Specialty Surgery Center, Region Östergötland, Linköping 58185, SwedenDepartment of Biomedical and Clinical Sciences, Division of Cell Biology, Linköping University, Linköping 58185, SwedenDepartment of Biomedical and Clinical Sciences, Division of Cell Biology, Linköping University, Linköping 58185, SwedenDepartment of Biomedical and Clinical Sciences, Division of Cell Biology, Linköping University, Linköping 58185, SwedenDepartment of Otorhinolaryngology in Linköping, Anaesthetics, Operations and Specialty Surgery Center, Region Östergötland, Linköping 58185, Sweden; Department of Biomedical and Clinical Sciences, Division of Cell Biology, Linköping University, Linköping 58185, Sweden; Corresponding authors.Department of Otorhinolaryngology in Linköping, Anaesthetics, Operations and Specialty Surgery Center, Region Östergötland, Linköping 58185, Sweden; Department of Biomedical and Clinical Sciences, Division of Cell Biology, Linköping University, Linköping 58185, Sweden; Corresponding authors.Head and neck squamous cell carcinoma (HNSCC) is a disease associated with a severe mortality and high risk of distant metastasis and local recurrence. Currently, surgery and radiotherapy are the main treatment modes, however, therapeutic efficacy of radiotherapy is linked to tumor resistance. Hypoxia has been shown to affect outcome of radiotherapy in HNSCC patients. The aim of this study was to verify the expression of the previously identified hypoxia-responsive genes (CA9, CASP14, LOX, GLUT3, SERPINE1, AREG, EREG, CCNB1 and KIF14) in HNSCC patient material as well as assess their prognostic potential. Tumor biopsies obtained before start of radiotherapy from 32 HNSCC patients classified as responders or non-responders were investigated in this study. The mRNA expression was quantified using RT-qPCR. The mRNA expression of CA9, SERPINE1 and KIF14 was significantly higher in the analyzed patient material compared with the non-cancerous oral tissue. Moreover, the KIF14 mRNA expression was significantly higher in the responder group compared to non-responders. Further studies demonstrated that knockdown of KIF14 reverses its radiosensitizing capability. Additionally, low expression of KIF14 mRNA correlated with significantly shorter OS (overall survival). In conclusion, our results suggest that KIF14 might be a useful prognostic and predictive marker in HNSCC.http://www.sciencedirect.com/science/article/pii/S1936523323002279Head and neck cancerHypoxiaRadiotherapyBiomarkerPrognostic factorKIF14 |
| spellingShingle | Natasa Matic Lina Pettersson Felicia Sellebjerg Lina Lindberg Karin Roberg Emilia Wiechec Prognostic value of hypoxia-responsive gene expression profile in patients diagnosed with head and neck squamous cell carcinoma Translational Oncology Head and neck cancer Hypoxia Radiotherapy Biomarker Prognostic factor KIF14 |
| title | Prognostic value of hypoxia-responsive gene expression profile in patients diagnosed with head and neck squamous cell carcinoma |
| title_full | Prognostic value of hypoxia-responsive gene expression profile in patients diagnosed with head and neck squamous cell carcinoma |
| title_fullStr | Prognostic value of hypoxia-responsive gene expression profile in patients diagnosed with head and neck squamous cell carcinoma |
| title_full_unstemmed | Prognostic value of hypoxia-responsive gene expression profile in patients diagnosed with head and neck squamous cell carcinoma |
| title_short | Prognostic value of hypoxia-responsive gene expression profile in patients diagnosed with head and neck squamous cell carcinoma |
| title_sort | prognostic value of hypoxia responsive gene expression profile in patients diagnosed with head and neck squamous cell carcinoma |
| topic | Head and neck cancer Hypoxia Radiotherapy Biomarker Prognostic factor KIF14 |
| url | http://www.sciencedirect.com/science/article/pii/S1936523323002279 |
| work_keys_str_mv | AT natasamatic prognosticvalueofhypoxiaresponsivegeneexpressionprofileinpatientsdiagnosedwithheadandnecksquamouscellcarcinoma AT linapettersson prognosticvalueofhypoxiaresponsivegeneexpressionprofileinpatientsdiagnosedwithheadandnecksquamouscellcarcinoma AT feliciasellebjerg prognosticvalueofhypoxiaresponsivegeneexpressionprofileinpatientsdiagnosedwithheadandnecksquamouscellcarcinoma AT linalindberg prognosticvalueofhypoxiaresponsivegeneexpressionprofileinpatientsdiagnosedwithheadandnecksquamouscellcarcinoma AT karinroberg prognosticvalueofhypoxiaresponsivegeneexpressionprofileinpatientsdiagnosedwithheadandnecksquamouscellcarcinoma AT emiliawiechec prognosticvalueofhypoxiaresponsivegeneexpressionprofileinpatientsdiagnosedwithheadandnecksquamouscellcarcinoma |