Effect of N-acetyl-L-cysteine on Testicular Tissue in Busulfan-Induced Dysfunction in the Male Reproductive System
Purpose: This study aimed to evaluate the protective effect of N-acetyl-L-cysteine (NAC) as an antioxidant on busulfaninduced testicular dysfunction in mice and elucidate its possible mechanism of action. Materials and Methods: Thirty-two C57BL/6 male mice were randomly divided into four groups (n...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Korean Society for Sexual Medicine and Andrology
2023-10-01
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| Series: | The World Journal of Men's Health |
| Subjects: |
| _version_ | 1827819058905481216 |
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| author | Kyung Hwan Kim Min Jung Park Nam Cheol Park Hyun Jun Park |
| author_facet | Kyung Hwan Kim Min Jung Park Nam Cheol Park Hyun Jun Park |
| author_sort | Kyung Hwan Kim |
| collection | DOAJ |
| description | Purpose: This study aimed to evaluate the protective effect of N-acetyl-L-cysteine (NAC) as an antioxidant on busulfaninduced
testicular dysfunction in mice and elucidate its possible mechanism of action.
Materials and Methods: Thirty-two C57BL/6 male mice were randomly divided into four groups (n=8/group) as follows: (1) control
group (oral administration of saline [0.1 mL daily] for 35 days); (2) NAC group (oral administration of NAC [10 mg/kg daily]
for 35 days); (3) busulfan group (double intraperitoneal injections of 20 mg/kg; total dose of 40 mg/kg); and (4) busulfan+NAC
group (after double intraperitoneal injections of 20 mg/kg; total dose of 40 mg/kg, NAC administration [10 mg/kg daily] for 35
days). The testes were removed, weighed, and subjected to sperm parameter analysis and morphology assessment. Reproductive
hormone, serum/testicular reactive oxygen species (ROS) level, oxidative stress and antioxidant markers were evaluated. The
testicular expression of Nrf2 and HO-1 was examined using RT-qPCR.
Results: Busulfan treatment significantly decreased testicular weight, sperm count, and serum testosterone levels. Atrophy
and degeneration of germinal epithelium were observed in the busulfan group. NAC administration after busulfan treatment
partially attenuated the deterioration of testis weight, sperm quality, serum hormones, histomorphometric changes, and oxidative
and antioxidative status. NAC treatment resulted in a considerable improvement in Nrf2 and HO-1 mRNA expression
levels.
Conclusions: This study provides compelling evidence that NAC as a potent antioxidant has significant protective effects
against busulfan-induced male reproductive impairment possibly through modification of the Nrf2/HO-1 signaling pathway. |
| first_indexed | 2024-03-12T01:07:45Z |
| format | Article |
| id | doaj.art-54e56df00fb74d589483f538725c0edb |
| institution | Directory Open Access Journal |
| issn | 2287-4208 2287-4690 |
| language | English |
| last_indexed | 2024-03-12T01:07:45Z |
| publishDate | 2023-10-01 |
| publisher | Korean Society for Sexual Medicine and Andrology |
| record_format | Article |
| series | The World Journal of Men's Health |
| spelling | doaj.art-54e56df00fb74d589483f538725c0edb2023-09-14T08:42:52ZengKorean Society for Sexual Medicine and AndrologyThe World Journal of Men's Health2287-42082287-46902023-10-0141488289110.5534/wjmh.220100Effect of N-acetyl-L-cysteine on Testicular Tissue in Busulfan-Induced Dysfunction in the Male Reproductive SystemKyung Hwan Kim0https://orcid.org/0000-0001-7162-6527Min Jung Park1https://orcid.org/0000-0002-9205-078XNam Cheol Park2https://orcid.org/0000-0003-2735-9278Hyun Jun Park3https://orcid.org/0000-0003-0566-9574Department of Urology, Pusan National University School of Medicine, Busan, KoreaThe Korea Institute for Public Sperm Bank, Busan, KoreaDepartment of Urology, Pusan National University School of Medicine, Busan, KoreaDepartment of Urology, Pusan National University School of Medicine, Busan, KoreaPurpose: This study aimed to evaluate the protective effect of N-acetyl-L-cysteine (NAC) as an antioxidant on busulfaninduced testicular dysfunction in mice and elucidate its possible mechanism of action. Materials and Methods: Thirty-two C57BL/6 male mice were randomly divided into four groups (n=8/group) as follows: (1) control group (oral administration of saline [0.1 mL daily] for 35 days); (2) NAC group (oral administration of NAC [10 mg/kg daily] for 35 days); (3) busulfan group (double intraperitoneal injections of 20 mg/kg; total dose of 40 mg/kg); and (4) busulfan+NAC group (after double intraperitoneal injections of 20 mg/kg; total dose of 40 mg/kg, NAC administration [10 mg/kg daily] for 35 days). The testes were removed, weighed, and subjected to sperm parameter analysis and morphology assessment. Reproductive hormone, serum/testicular reactive oxygen species (ROS) level, oxidative stress and antioxidant markers were evaluated. The testicular expression of Nrf2 and HO-1 was examined using RT-qPCR. Results: Busulfan treatment significantly decreased testicular weight, sperm count, and serum testosterone levels. Atrophy and degeneration of germinal epithelium were observed in the busulfan group. NAC administration after busulfan treatment partially attenuated the deterioration of testis weight, sperm quality, serum hormones, histomorphometric changes, and oxidative and antioxidative status. NAC treatment resulted in a considerable improvement in Nrf2 and HO-1 mRNA expression levels. Conclusions: This study provides compelling evidence that NAC as a potent antioxidant has significant protective effects against busulfan-induced male reproductive impairment possibly through modification of the Nrf2/HO-1 signaling pathway.acetylcysteineantioxidant effectbusulfanmousereproductive systemtestis |
| spellingShingle | Kyung Hwan Kim Min Jung Park Nam Cheol Park Hyun Jun Park Effect of N-acetyl-L-cysteine on Testicular Tissue in Busulfan-Induced Dysfunction in the Male Reproductive System The World Journal of Men's Health acetylcysteine antioxidant effect busulfan mouse reproductive system testis |
| title | Effect of N-acetyl-L-cysteine on Testicular Tissue in Busulfan-Induced Dysfunction in the Male Reproductive System |
| title_full | Effect of N-acetyl-L-cysteine on Testicular Tissue in Busulfan-Induced Dysfunction in the Male Reproductive System |
| title_fullStr | Effect of N-acetyl-L-cysteine on Testicular Tissue in Busulfan-Induced Dysfunction in the Male Reproductive System |
| title_full_unstemmed | Effect of N-acetyl-L-cysteine on Testicular Tissue in Busulfan-Induced Dysfunction in the Male Reproductive System |
| title_short | Effect of N-acetyl-L-cysteine on Testicular Tissue in Busulfan-Induced Dysfunction in the Male Reproductive System |
| title_sort | effect of n acetyl l cysteine on testicular tissue in busulfan induced dysfunction in the male reproductive system |
| topic | acetylcysteine antioxidant effect busulfan mouse reproductive system testis |
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