Apoptotic changes and aquaporin-1 expression in the choroid plexus of cerebral malaria patients
Abstract Background Cerebral malaria (CM) is associated with sequestration of parasitized red blood cells (PRBCs) in the capillaries. Often, the association of CM with cerebral oedema is related with high mortality rate. Morphological changes of the choroid plexus (CP) and caspase-3 expression in CM...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-02-01
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| Series: | Malaria Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12936-022-04044-6 |
| _version_ | 1818330642129092608 |
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| author | Charit Srisook Supattra Glaharn Chuchard Punsawad Parnpen Viriyavejakul |
| author_facet | Charit Srisook Supattra Glaharn Chuchard Punsawad Parnpen Viriyavejakul |
| author_sort | Charit Srisook |
| collection | DOAJ |
| description | Abstract Background Cerebral malaria (CM) is associated with sequestration of parasitized red blood cells (PRBCs) in the capillaries. Often, the association of CM with cerebral oedema is related with high mortality rate. Morphological changes of the choroid plexus (CP) and caspase-3 expression in CM have not been reported. In addition, limited knowledge is known regarding the role of aquaporin (AQP)-1 in CM. The present study evaluated changes in the CP, explored apoptotic changes and AQP-1 expression in CP epithelial cells (CPECs) in fatal CM patients. Methods CP from fatal Plasmodium falciparum malaria patients (5 non-CM [NCM], 16 CM) were retrieved and prepared for histopathological evaluation. Caspase-3 and AQP-1 expressions in CPECs were investigated by immunohistochemistry. Results Histologically, apoptotic changes in CPECs were significantly observed in the CM group compared with the NCM and normal control (NC) groups (p < 0.05). These changes included cytoplasmic and nuclear condensation/shrinkage of CPECs and detachment of CPECs from the basement membrane. The apoptotic changes were positively correlated with caspase-3 expression in the nuclei of CPECs. In addition, AQP-1 expression in CPECs was significantly decreased in the CM group compared with the NCM and NC groups (all p < 0.001). A negative correlation (r s = − 0.450, p = 0.024) was documented between caspase-3 expression in the nuclei of CPECs and AQP-1. Conclusions Apoptotic changes and altered AQP-1 expression may contribute to CPEC dysfunction and subsequently reduce cerebrospinal fluid production, affecting the water homeostasis in the brains of patients with CM. |
| first_indexed | 2024-12-13T13:07:12Z |
| format | Article |
| id | doaj.art-54e7326e09ca4ac8ac62de4bccad02fe |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-13T13:07:12Z |
| publishDate | 2022-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-54e7326e09ca4ac8ac62de4bccad02fe2022-12-21T23:44:47ZengBMCMalaria Journal1475-28752022-02-0121111010.1186/s12936-022-04044-6Apoptotic changes and aquaporin-1 expression in the choroid plexus of cerebral malaria patientsCharit Srisook0Supattra Glaharn1Chuchard Punsawad2Parnpen Viriyavejakul3Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol UniversityDepartment of Tropical Pathology, Faculty of Tropical Medicine, Mahidol UniversitySchool of Medicine, Walailak UniversityDepartment of Tropical Pathology, Faculty of Tropical Medicine, Mahidol UniversityAbstract Background Cerebral malaria (CM) is associated with sequestration of parasitized red blood cells (PRBCs) in the capillaries. Often, the association of CM with cerebral oedema is related with high mortality rate. Morphological changes of the choroid plexus (CP) and caspase-3 expression in CM have not been reported. In addition, limited knowledge is known regarding the role of aquaporin (AQP)-1 in CM. The present study evaluated changes in the CP, explored apoptotic changes and AQP-1 expression in CP epithelial cells (CPECs) in fatal CM patients. Methods CP from fatal Plasmodium falciparum malaria patients (5 non-CM [NCM], 16 CM) were retrieved and prepared for histopathological evaluation. Caspase-3 and AQP-1 expressions in CPECs were investigated by immunohistochemistry. Results Histologically, apoptotic changes in CPECs were significantly observed in the CM group compared with the NCM and normal control (NC) groups (p < 0.05). These changes included cytoplasmic and nuclear condensation/shrinkage of CPECs and detachment of CPECs from the basement membrane. The apoptotic changes were positively correlated with caspase-3 expression in the nuclei of CPECs. In addition, AQP-1 expression in CPECs was significantly decreased in the CM group compared with the NCM and NC groups (all p < 0.001). A negative correlation (r s = − 0.450, p = 0.024) was documented between caspase-3 expression in the nuclei of CPECs and AQP-1. Conclusions Apoptotic changes and altered AQP-1 expression may contribute to CPEC dysfunction and subsequently reduce cerebrospinal fluid production, affecting the water homeostasis in the brains of patients with CM.https://doi.org/10.1186/s12936-022-04044-6Cerebral malariaChoroid plexusP. falciparumAquaporin-1AQP-1Caspase-3 |
| spellingShingle | Charit Srisook Supattra Glaharn Chuchard Punsawad Parnpen Viriyavejakul Apoptotic changes and aquaporin-1 expression in the choroid plexus of cerebral malaria patients Malaria Journal Cerebral malaria Choroid plexus P. falciparum Aquaporin-1 AQP-1 Caspase-3 |
| title | Apoptotic changes and aquaporin-1 expression in the choroid plexus of cerebral malaria patients |
| title_full | Apoptotic changes and aquaporin-1 expression in the choroid plexus of cerebral malaria patients |
| title_fullStr | Apoptotic changes and aquaporin-1 expression in the choroid plexus of cerebral malaria patients |
| title_full_unstemmed | Apoptotic changes and aquaporin-1 expression in the choroid plexus of cerebral malaria patients |
| title_short | Apoptotic changes and aquaporin-1 expression in the choroid plexus of cerebral malaria patients |
| title_sort | apoptotic changes and aquaporin 1 expression in the choroid plexus of cerebral malaria patients |
| topic | Cerebral malaria Choroid plexus P. falciparum Aquaporin-1 AQP-1 Caspase-3 |
| url | https://doi.org/10.1186/s12936-022-04044-6 |
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