Protocol for cell-type-specific tissue reconstruction in the murine lung fibrogenic microenvironment
Summary: Pulmonary fibrosis is a process characterized by epithelial injury and fibroblast activation. It is also well recognized as a predisposition to lung cancer. Here, we present a protocol to establish an in vivo model to evaluate the dynamics of alveolar epithelial type 2 cells and lung cancer...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672200898X |
| _version_ | 1797942040487526400 |
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| author | Koichi Hasegawa Ryo Miyata Toshi Menju Toyohiro Hirai Hiroshi Date Atsuyasu Sato |
| author_facet | Koichi Hasegawa Ryo Miyata Toshi Menju Toyohiro Hirai Hiroshi Date Atsuyasu Sato |
| author_sort | Koichi Hasegawa |
| collection | DOAJ |
| description | Summary: Pulmonary fibrosis is a process characterized by epithelial injury and fibroblast activation. It is also well recognized as a predisposition to lung cancer. Here, we present a protocol to establish an in vivo model to evaluate the dynamics of alveolar epithelial type 2 cells and lung cancer cells in the context of the lung fibrogenic microenvironment. Utilizing the cell transfer technique, we detail a basis for therapeutic approaches in pulmonary fibrosis and tools for precision medicine against lung cancer.For complete details on the use and execution of this protocol, please refer to Miyata et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-10T20:02:27Z |
| format | Article |
| id | doaj.art-54eb6a290f9a4cbab4937b826130c450 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-10T20:02:27Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-54eb6a290f9a4cbab4937b826130c4502023-01-27T04:20:53ZengElsevierSTAR Protocols2666-16672023-03-0141102018Protocol for cell-type-specific tissue reconstruction in the murine lung fibrogenic microenvironmentKoichi Hasegawa0Ryo Miyata1Toshi Menju2Toyohiro Hirai3Hiroshi Date4Atsuyasu Sato5Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan; Corresponding authorDepartment of Thoracic Surgery, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, JapanDepartment of Thoracic Surgery, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, JapanDepartment of Respiratory Medicine, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, JapanDepartment of Thoracic Surgery, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, JapanDepartment of Respiratory Medicine, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan; Corresponding authorSummary: Pulmonary fibrosis is a process characterized by epithelial injury and fibroblast activation. It is also well recognized as a predisposition to lung cancer. Here, we present a protocol to establish an in vivo model to evaluate the dynamics of alveolar epithelial type 2 cells and lung cancer cells in the context of the lung fibrogenic microenvironment. Utilizing the cell transfer technique, we detail a basis for therapeutic approaches in pulmonary fibrosis and tools for precision medicine against lung cancer.For complete details on the use and execution of this protocol, please refer to Miyata et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S266616672200898XCell isolationCancerCell DifferentiationTissue Engineering |
| spellingShingle | Koichi Hasegawa Ryo Miyata Toshi Menju Toyohiro Hirai Hiroshi Date Atsuyasu Sato Protocol for cell-type-specific tissue reconstruction in the murine lung fibrogenic microenvironment STAR Protocols Cell isolation Cancer Cell Differentiation Tissue Engineering |
| title | Protocol for cell-type-specific tissue reconstruction in the murine lung fibrogenic microenvironment |
| title_full | Protocol for cell-type-specific tissue reconstruction in the murine lung fibrogenic microenvironment |
| title_fullStr | Protocol for cell-type-specific tissue reconstruction in the murine lung fibrogenic microenvironment |
| title_full_unstemmed | Protocol for cell-type-specific tissue reconstruction in the murine lung fibrogenic microenvironment |
| title_short | Protocol for cell-type-specific tissue reconstruction in the murine lung fibrogenic microenvironment |
| title_sort | protocol for cell type specific tissue reconstruction in the murine lung fibrogenic microenvironment |
| topic | Cell isolation Cancer Cell Differentiation Tissue Engineering |
| url | http://www.sciencedirect.com/science/article/pii/S266616672200898X |
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