Impact of adding different concentrations of IGF-I and insulin to the semen extender on bull sperm quality post-cryopreservation
ABSTRACT This study aimed to evaluate the addition of different concentrations of IGF-I and insulin to egg yolk-based extender to improve bovine semen cryopreservation. Two experiments were developed to evaluate the effects of the additives in two commercial extenders, Botubov® (Experiment 1) and Tr...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Minas Gerais
2023-09-01
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352023000500771&lng=en&tlng=en |
| _version_ | 1827814733550452736 |
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| author | S.C.C. Pinto F.A. Souza R.P. Arruda M.B.R. Alves L. Batissaco L.N. Garcia-Oliveros M.S. Passarelli L.C. Carneiro E.C.C. Celeghini |
| author_facet | S.C.C. Pinto F.A. Souza R.P. Arruda M.B.R. Alves L. Batissaco L.N. Garcia-Oliveros M.S. Passarelli L.C. Carneiro E.C.C. Celeghini |
| author_sort | S.C.C. Pinto |
| collection | DOAJ |
| description | ABSTRACT This study aimed to evaluate the addition of different concentrations of IGF-I and insulin to egg yolk-based extender to improve bovine semen cryopreservation. Two experiments were developed to evaluate the effects of the additives in two commercial extenders, Botubov® (Experiment 1) and Triladyl® (Experiment 2), both with the same design. Three ejaculates from four bulls (n = 12) were used. Each ejaculate was divided into seven equal fractions for dilution (60x106 spermatozoa/mL) in the following treatments: CON: extender only; IGF100: IGF-I 100ng/mL; IGF200: IGF-I 200ng/mL; INS150: insulin 150µUI/mL; INS200: insulin 200µUI/mL; ASS1: IGF-I 100ng/mL + insulin 150µUI/mL; ASS2: IGF-I 200ng/mL + insulin 200µUI/mL. Semen was cryopreserved by an automated system. Post-thawed sperm were evaluated regarding motility by CASA (Computer-assisted sperm analysis), and membranes by fluorescent probes (H342, PI, FITC-PSA and JC-1). For Botubov® extender, INS150 was more efficient in preserving total and progressive motility, VCL, BCF, plasma and mitochondrial membranes. A similar response was seen when insulin was added to the Triladyl® extender, INS150 was more efficient in preserving sperm motility, plasma membrane integrity and mitochondrial potential. Thus, the addition of insulin 150µUI/mL, regardless of the composition of the extender, contributes to better preserving bovine sperm from the cryopreservation effects. |
| first_indexed | 2024-03-11T23:49:03Z |
| format | Article |
| id | doaj.art-54eda5b94c1e4e49867b9f40817cbccd |
| institution | Directory Open Access Journal |
| issn | 1678-4162 |
| language | English |
| last_indexed | 2024-03-11T23:49:03Z |
| publishDate | 2023-09-01 |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-54eda5b94c1e4e49867b9f40817cbccd2023-09-19T07:45:49ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622023-09-0175577178610.1590/1678-4162-12946Impact of adding different concentrations of IGF-I and insulin to the semen extender on bull sperm quality post-cryopreservationS.C.C. Pintohttps://orcid.org/0000-0002-4220-3186F.A. Souzahttps://orcid.org/0000-0002-9474-9404R.P. Arrudahttps://orcid.org/0000-0003-0893-8098M.B.R. Alveshttps://orcid.org/0000-0002-9480-6608L. Batissacohttps://orcid.org/0000-0001-8041-6732L.N. Garcia-Oliveroshttps://orcid.org/0000-0001-7093-5707M.S. Passarellihttps://orcid.org/0000-0001-6138-4587L.C. Carneirohttps://orcid.org/0000-0002-5324-010XE.C.C. Celeghinihttps://orcid.org/0000-0003-1531-5497ABSTRACT This study aimed to evaluate the addition of different concentrations of IGF-I and insulin to egg yolk-based extender to improve bovine semen cryopreservation. Two experiments were developed to evaluate the effects of the additives in two commercial extenders, Botubov® (Experiment 1) and Triladyl® (Experiment 2), both with the same design. Three ejaculates from four bulls (n = 12) were used. Each ejaculate was divided into seven equal fractions for dilution (60x106 spermatozoa/mL) in the following treatments: CON: extender only; IGF100: IGF-I 100ng/mL; IGF200: IGF-I 200ng/mL; INS150: insulin 150µUI/mL; INS200: insulin 200µUI/mL; ASS1: IGF-I 100ng/mL + insulin 150µUI/mL; ASS2: IGF-I 200ng/mL + insulin 200µUI/mL. Semen was cryopreserved by an automated system. Post-thawed sperm were evaluated regarding motility by CASA (Computer-assisted sperm analysis), and membranes by fluorescent probes (H342, PI, FITC-PSA and JC-1). For Botubov® extender, INS150 was more efficient in preserving total and progressive motility, VCL, BCF, plasma and mitochondrial membranes. A similar response was seen when insulin was added to the Triladyl® extender, INS150 was more efficient in preserving sperm motility, plasma membrane integrity and mitochondrial potential. Thus, the addition of insulin 150µUI/mL, regardless of the composition of the extender, contributes to better preserving bovine sperm from the cryopreservation effects.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352023000500771&lng=en&tlng=enBullSemenFreezingBotubov®Triladyl® |
| spellingShingle | S.C.C. Pinto F.A. Souza R.P. Arruda M.B.R. Alves L. Batissaco L.N. Garcia-Oliveros M.S. Passarelli L.C. Carneiro E.C.C. Celeghini Impact of adding different concentrations of IGF-I and insulin to the semen extender on bull sperm quality post-cryopreservation Arquivo Brasileiro de Medicina Veterinária e Zootecnia Bull Semen Freezing Botubov® Triladyl® |
| title | Impact of adding different concentrations of IGF-I and insulin to the semen extender on bull sperm quality post-cryopreservation |
| title_full | Impact of adding different concentrations of IGF-I and insulin to the semen extender on bull sperm quality post-cryopreservation |
| title_fullStr | Impact of adding different concentrations of IGF-I and insulin to the semen extender on bull sperm quality post-cryopreservation |
| title_full_unstemmed | Impact of adding different concentrations of IGF-I and insulin to the semen extender on bull sperm quality post-cryopreservation |
| title_short | Impact of adding different concentrations of IGF-I and insulin to the semen extender on bull sperm quality post-cryopreservation |
| title_sort | impact of adding different concentrations of igf i and insulin to the semen extender on bull sperm quality post cryopreservation |
| topic | Bull Semen Freezing Botubov® Triladyl® |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352023000500771&lng=en&tlng=en |
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