Advances in the diagnosis of the gastrointestinal nematode infections in ruminants
Enumeration of nematode eggs in fecal samples using the McMaster technique and morphological identification of third stage larvae from fecal cultures have been extensively used with satisfactory results in the diagnosis of the gastrointestinal nematode infections in ruminants. In order to improve se...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2016-06-01
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| Series: | Brazilian Journal of Veterinary Research and Animal Science |
| Subjects: | |
| Online Access: | http://www.revistas.usp.br/bjvras/article/view/109785 |
| _version_ | 1819088179485999104 |
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| author | Alessandro Francisco Talamini do Amarante Mônica Regina Vendrame Amarante |
| author_facet | Alessandro Francisco Talamini do Amarante Mônica Regina Vendrame Amarante |
| author_sort | Alessandro Francisco Talamini do Amarante |
| collection | DOAJ |
| description | Enumeration of nematode eggs in fecal samples using the McMaster technique and morphological identification of third stage larvae from fecal cultures have been extensively used with satisfactory results in the diagnosis of the gastrointestinal nematode infections in ruminants. In order to improve sensitivity and accuracy, other approaches for quantification of eggs have been employed, like the FLOTAC and Mini-FLOTAC techniques. Results obtained in different studies indicate that fecal egg counts are a reliable measure of the size of the worm burden. However, the immunological status of the animals should be taken into consideration to interpret the results of the fecal examination. Molecular techniques have also been useful in the diagnosis of parasitic diseases. The ultimate in diagnosis has been the development of robotic platforms that enable separation of eggs from feces. Because manipulation is minimal, good quality DNA from eggs is obtained, which is used for amplification, and finally, produces a result indicating the degree of the infection by the different parasite species in mix infections. The ideal method should be reliable, friendly to non-experts and quick to perform. With the advance in robotics, bioinformatics and molecular biology, methods with such characteristics are expected to become available and affordable to be used in laboratories for the routine diagnosis of gastrointestinal nematodes of ruminants. |
| first_indexed | 2024-12-21T21:47:56Z |
| format | Article |
| id | doaj.art-54ef134c02df4ff1ba66fa3a7fe65018 |
| institution | Directory Open Access Journal |
| issn | 1413-9596 1678-4456 |
| language | English |
| last_indexed | 2024-12-21T21:47:56Z |
| publishDate | 2016-06-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Veterinary Research and Animal Science |
| spelling | doaj.art-54ef134c02df4ff1ba66fa3a7fe650182022-12-21T18:49:10ZengUniversidade de São PauloBrazilian Journal of Veterinary Research and Animal Science1413-95961678-44562016-06-0153210.11606/issn.1678-4456.v53i2p127-137101898Advances in the diagnosis of the gastrointestinal nematode infections in ruminantsAlessandro Francisco Talamini do Amarante0Mônica Regina Vendrame Amarante1Universidade Estadual Paulista, Instituto de Biociências, Departamento de Parasitologia, BotucatuUniversidade Estadual Paulista, Instituto de Biociências, Departamento de Parasitologia, BotucatuEnumeration of nematode eggs in fecal samples using the McMaster technique and morphological identification of third stage larvae from fecal cultures have been extensively used with satisfactory results in the diagnosis of the gastrointestinal nematode infections in ruminants. In order to improve sensitivity and accuracy, other approaches for quantification of eggs have been employed, like the FLOTAC and Mini-FLOTAC techniques. Results obtained in different studies indicate that fecal egg counts are a reliable measure of the size of the worm burden. However, the immunological status of the animals should be taken into consideration to interpret the results of the fecal examination. Molecular techniques have also been useful in the diagnosis of parasitic diseases. The ultimate in diagnosis has been the development of robotic platforms that enable separation of eggs from feces. Because manipulation is minimal, good quality DNA from eggs is obtained, which is used for amplification, and finally, produces a result indicating the degree of the infection by the different parasite species in mix infections. The ideal method should be reliable, friendly to non-experts and quick to perform. With the advance in robotics, bioinformatics and molecular biology, methods with such characteristics are expected to become available and affordable to be used in laboratories for the routine diagnosis of gastrointestinal nematodes of ruminants.http://www.revistas.usp.br/bjvras/article/view/109785HaemonchusCooperiaEPGPCR |
| spellingShingle | Alessandro Francisco Talamini do Amarante Mônica Regina Vendrame Amarante Advances in the diagnosis of the gastrointestinal nematode infections in ruminants Brazilian Journal of Veterinary Research and Animal Science Haemonchus Cooperia EPG PCR |
| title | Advances in the diagnosis of the gastrointestinal nematode infections in ruminants |
| title_full | Advances in the diagnosis of the gastrointestinal nematode infections in ruminants |
| title_fullStr | Advances in the diagnosis of the gastrointestinal nematode infections in ruminants |
| title_full_unstemmed | Advances in the diagnosis of the gastrointestinal nematode infections in ruminants |
| title_short | Advances in the diagnosis of the gastrointestinal nematode infections in ruminants |
| title_sort | advances in the diagnosis of the gastrointestinal nematode infections in ruminants |
| topic | Haemonchus Cooperia EPG PCR |
| url | http://www.revistas.usp.br/bjvras/article/view/109785 |
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