PI4KB on Inclusion Bodies Formed by ER Membrane Remodeling Facilitates Replication of Human Parainfluenza Virus Type 3
Summary: Many positive-strand RNA viruses remodel the endomembrane to form specialized replication organelles. However, knowledge regarding whether negative-strand RNA viruses take advantage of intracellular membranes for replication is limited. Here we show that a negative-strand RNA virus, human p...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-11-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221112471931366X |
| _version_ | 1811335674745323520 |
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| author | Zhifei Li Dong Guo Yali Qin Mingzhou Chen |
| author_facet | Zhifei Li Dong Guo Yali Qin Mingzhou Chen |
| author_sort | Zhifei Li |
| collection | DOAJ |
| description | Summary: Many positive-strand RNA viruses remodel the endomembrane to form specialized replication organelles. However, knowledge regarding whether negative-strand RNA viruses take advantage of intracellular membranes for replication is limited. Here we show that a negative-strand RNA virus, human parainfluenza virus type 3 (HPIV3), remodels the endoplasmic reticulum (ER) membrane to form inclusion bodies (IBs), whereby the phosphoprotein (P) of HPIV3 recruits phosphatidylinositol 4-kinase beta (PI4KB) to IBs to generate PI4P, creating a PI4P-enriched microenvironment to promote HPIV3 replication. In addition, we find that human respiratory syncytial virus (HRSV) also takes advantage of the ER to form IBs and that these IBs are also enriched with PI4P. The nucleoprotein of HRSV recruits PI4KB to IBs. These results suggest that paramyxoviruses also exploit the host endomembrane to form IBs and that PI4KB is recruited by viral proteins to enrich IBs with PI4P to facilitate viral replication. : Many positive-strand RNA viruses reorganize the endomembrane to form specialized replication organelles. However, whether negative-strand RNA viruses use intracellular membranes for replication is largely unknown. Li et al. demonstrate that inclusion bodies of HPIV3 induce ER membrane rearrangement. PI4KB is recruited to IBs to generate PI4P, which facilitates viral replication. Keywords: PI4KB, IBs, ER membrane, HPIV3 |
| first_indexed | 2024-04-13T17:27:46Z |
| format | Article |
| id | doaj.art-54ef3e3c1dae42a18b6cb38e58af2273 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-04-13T17:27:46Z |
| publishDate | 2019-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-54ef3e3c1dae42a18b6cb38e58af22732022-12-22T02:37:41ZengElsevierCell Reports2211-12472019-11-0129822292242.e4PI4KB on Inclusion Bodies Formed by ER Membrane Remodeling Facilitates Replication of Human Parainfluenza Virus Type 3Zhifei Li0Dong Guo1Yali Qin2Mingzhou Chen3State Key Laboratory of Virology and Modern Virology Research Center, College of Life Sciences, Wuhan University, LuoJia Hill, Wuhan 430072, ChinaState Key Laboratory of Virology and Modern Virology Research Center, College of Life Sciences, Wuhan University, LuoJia Hill, Wuhan 430072, ChinaState Key Laboratory of Virology and Modern Virology Research Center, College of Life Sciences, Wuhan University, LuoJia Hill, Wuhan 430072, China; Corresponding authorState Key Laboratory of Virology and Modern Virology Research Center, College of Life Sciences, Wuhan University, LuoJia Hill, Wuhan 430072, China; Corresponding authorSummary: Many positive-strand RNA viruses remodel the endomembrane to form specialized replication organelles. However, knowledge regarding whether negative-strand RNA viruses take advantage of intracellular membranes for replication is limited. Here we show that a negative-strand RNA virus, human parainfluenza virus type 3 (HPIV3), remodels the endoplasmic reticulum (ER) membrane to form inclusion bodies (IBs), whereby the phosphoprotein (P) of HPIV3 recruits phosphatidylinositol 4-kinase beta (PI4KB) to IBs to generate PI4P, creating a PI4P-enriched microenvironment to promote HPIV3 replication. In addition, we find that human respiratory syncytial virus (HRSV) also takes advantage of the ER to form IBs and that these IBs are also enriched with PI4P. The nucleoprotein of HRSV recruits PI4KB to IBs. These results suggest that paramyxoviruses also exploit the host endomembrane to form IBs and that PI4KB is recruited by viral proteins to enrich IBs with PI4P to facilitate viral replication. : Many positive-strand RNA viruses reorganize the endomembrane to form specialized replication organelles. However, whether negative-strand RNA viruses use intracellular membranes for replication is largely unknown. Li et al. demonstrate that inclusion bodies of HPIV3 induce ER membrane rearrangement. PI4KB is recruited to IBs to generate PI4P, which facilitates viral replication. Keywords: PI4KB, IBs, ER membrane, HPIV3http://www.sciencedirect.com/science/article/pii/S221112471931366X |
| spellingShingle | Zhifei Li Dong Guo Yali Qin Mingzhou Chen PI4KB on Inclusion Bodies Formed by ER Membrane Remodeling Facilitates Replication of Human Parainfluenza Virus Type 3 Cell Reports |
| title | PI4KB on Inclusion Bodies Formed by ER Membrane Remodeling Facilitates Replication of Human Parainfluenza Virus Type 3 |
| title_full | PI4KB on Inclusion Bodies Formed by ER Membrane Remodeling Facilitates Replication of Human Parainfluenza Virus Type 3 |
| title_fullStr | PI4KB on Inclusion Bodies Formed by ER Membrane Remodeling Facilitates Replication of Human Parainfluenza Virus Type 3 |
| title_full_unstemmed | PI4KB on Inclusion Bodies Formed by ER Membrane Remodeling Facilitates Replication of Human Parainfluenza Virus Type 3 |
| title_short | PI4KB on Inclusion Bodies Formed by ER Membrane Remodeling Facilitates Replication of Human Parainfluenza Virus Type 3 |
| title_sort | pi4kb on inclusion bodies formed by er membrane remodeling facilitates replication of human parainfluenza virus type 3 |
| url | http://www.sciencedirect.com/science/article/pii/S221112471931366X |
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