Long-Term Analysis of Resilience of the Oral Microbiome in Allogeneic Stem Cell Transplant Recipients
Stem cell transplantation (SCT) is associated with oral microbial dysbiosis. However, long-term longitudinal data are lacking. Therefore, this study aimed to longitudinally assess the oral microbiome in SCT patients and to determine if changes are associated with oral mucositis and oral chronic graf...
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MDPI AG
2022-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/4/734 |
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| author | Alexa M. G. A. Laheij Frederik R. Rozema Michael T. Brennan Inger von Bültzingslöwen Stephanie J. M. van Leeuwen Carin Potting Marie-Charlotte D. N. J. M. Huysmans Mette D. Hazenberg Bernd W. Brandt Egija Zaura Mark J. Buijs Johannes J. de Soet Nicole N. M. Blijlevens Judith E. Raber-Durlacher |
| author_facet | Alexa M. G. A. Laheij Frederik R. Rozema Michael T. Brennan Inger von Bültzingslöwen Stephanie J. M. van Leeuwen Carin Potting Marie-Charlotte D. N. J. M. Huysmans Mette D. Hazenberg Bernd W. Brandt Egija Zaura Mark J. Buijs Johannes J. de Soet Nicole N. M. Blijlevens Judith E. Raber-Durlacher |
| author_sort | Alexa M. G. A. Laheij |
| collection | DOAJ |
| description | Stem cell transplantation (SCT) is associated with oral microbial dysbiosis. However, long-term longitudinal data are lacking. Therefore, this study aimed to longitudinally assess the oral microbiome in SCT patients and to determine if changes are associated with oral mucositis and oral chronic graft-versus-host disease. Fifty allogeneic SCT recipients treated in two Dutch university hospitals were prospectively followed, starting at pre-SCT, weekly during hospitalization, and at 3, 6, 12, and 18 months after SCT. Oral rinsing samples were taken, and oral mucositis (WHO score) and oral chronic graft-versus-host disease (NIH score) were assessed. The oral microbiome diversity (Shannon index) and composition significantly changed after SCT and returned to pre-treatment levels from 3 months after SCT. Oral mucositis was associated with a more pronounced decrease in microbial diversity and with several disease-associated genera, such as Mycobacterium, Staphylococcus, and Enterococcus. On the other hand, microbiome diversity and composition were not associated with oral chronic graft-versus-host disease. To conclude, dysbiosis of the oral microbiome occurred directly after SCT but recovered after 3 months. Diversity and composition were related to oral mucositis but not to oral chronic graft-versus-host disease. |
| first_indexed | 2024-03-09T04:22:51Z |
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| id | doaj.art-54efc5efdfcf43cf82f4b4fa5e6b9eec |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T04:22:51Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-54efc5efdfcf43cf82f4b4fa5e6b9eec2023-12-03T13:44:55ZengMDPI AGMicroorganisms2076-26072022-03-0110473410.3390/microorganisms10040734Long-Term Analysis of Resilience of the Oral Microbiome in Allogeneic Stem Cell Transplant RecipientsAlexa M. G. A. Laheij0Frederik R. Rozema1Michael T. Brennan2Inger von Bültzingslöwen3Stephanie J. M. van Leeuwen4Carin Potting5Marie-Charlotte D. N. J. M. Huysmans6Mette D. Hazenberg7Bernd W. Brandt8Egija Zaura9Mark J. Buijs10Johannes J. de Soet11Nicole N. M. Blijlevens12Judith E. Raber-Durlacher13Department of Oral Medicine, Academic Centre for Dentistry Amsterdam, University of Amsterdam and Vrije Universiteit Amsterdam, 1081 LA Amsterdam, The NetherlandsDepartment of Oral Medicine, Academic Centre for Dentistry Amsterdam, University of Amsterdam and Vrije Universiteit Amsterdam, 1081 LA Amsterdam, The NetherlandsDepartment of Oral Medicine, Atrium Health Carolinas Medical Centre, Charlotte, NC 28209, USADepartment of Oral Microbiology and Immunology, Institute of Odontology, The Sahlgrenska Academy, University of Gothenburg, 413 90 Gothenburg, SwedenDepartment of Dentistry, Radboud Institute for Health Sciences, Radboud University Medical Center, 6525 EX Nijmegen, The NetherlandsDepartment of Hematology, Radboud Institute for Health Sciences, Radboud University Medical Center, 6525 GA Nijmegen, The NetherlandsDepartment of Dentistry, Radboud Institute for Health Sciences, Radboud University Medical Center, 6525 EX Nijmegen, The NetherlandsDepartment of Hematology, Amsterdam UMC, University of Amsterdam, 1081 LA Amsterdam, The NetherlandsDepartment of Preventive Dentistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and Vrije Universiteit Amsterdam, 1081 LA Amsterdam, The NetherlandsDepartment of Preventive Dentistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and Vrije Universiteit Amsterdam, 1081 LA Amsterdam, The NetherlandsDepartment of Preventive Dentistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and Vrije Universiteit Amsterdam, 1081 LA Amsterdam, The NetherlandsDepartment of Preventive Dentistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and Vrije Universiteit Amsterdam, 1081 LA Amsterdam, The NetherlandsDepartment of Hematology, Radboud Institute for Health Sciences, Radboud University Medical Center, 6525 GA Nijmegen, The NetherlandsDepartment of Oral Medicine, Academic Centre for Dentistry Amsterdam, University of Amsterdam and Vrije Universiteit Amsterdam, 1081 LA Amsterdam, The NetherlandsStem cell transplantation (SCT) is associated with oral microbial dysbiosis. However, long-term longitudinal data are lacking. Therefore, this study aimed to longitudinally assess the oral microbiome in SCT patients and to determine if changes are associated with oral mucositis and oral chronic graft-versus-host disease. Fifty allogeneic SCT recipients treated in two Dutch university hospitals were prospectively followed, starting at pre-SCT, weekly during hospitalization, and at 3, 6, 12, and 18 months after SCT. Oral rinsing samples were taken, and oral mucositis (WHO score) and oral chronic graft-versus-host disease (NIH score) were assessed. The oral microbiome diversity (Shannon index) and composition significantly changed after SCT and returned to pre-treatment levels from 3 months after SCT. Oral mucositis was associated with a more pronounced decrease in microbial diversity and with several disease-associated genera, such as Mycobacterium, Staphylococcus, and Enterococcus. On the other hand, microbiome diversity and composition were not associated with oral chronic graft-versus-host disease. To conclude, dysbiosis of the oral microbiome occurred directly after SCT but recovered after 3 months. Diversity and composition were related to oral mucositis but not to oral chronic graft-versus-host disease.https://www.mdpi.com/2076-2607/10/4/734allogeneic stem cell transplantoral microbiomedysbiosisoral graft-versus-host diseaseoral mucositisconditioning |
| spellingShingle | Alexa M. G. A. Laheij Frederik R. Rozema Michael T. Brennan Inger von Bültzingslöwen Stephanie J. M. van Leeuwen Carin Potting Marie-Charlotte D. N. J. M. Huysmans Mette D. Hazenberg Bernd W. Brandt Egija Zaura Mark J. Buijs Johannes J. de Soet Nicole N. M. Blijlevens Judith E. Raber-Durlacher Long-Term Analysis of Resilience of the Oral Microbiome in Allogeneic Stem Cell Transplant Recipients Microorganisms allogeneic stem cell transplant oral microbiome dysbiosis oral graft-versus-host disease oral mucositis conditioning |
| title | Long-Term Analysis of Resilience of the Oral Microbiome in Allogeneic Stem Cell Transplant Recipients |
| title_full | Long-Term Analysis of Resilience of the Oral Microbiome in Allogeneic Stem Cell Transplant Recipients |
| title_fullStr | Long-Term Analysis of Resilience of the Oral Microbiome in Allogeneic Stem Cell Transplant Recipients |
| title_full_unstemmed | Long-Term Analysis of Resilience of the Oral Microbiome in Allogeneic Stem Cell Transplant Recipients |
| title_short | Long-Term Analysis of Resilience of the Oral Microbiome in Allogeneic Stem Cell Transplant Recipients |
| title_sort | long term analysis of resilience of the oral microbiome in allogeneic stem cell transplant recipients |
| topic | allogeneic stem cell transplant oral microbiome dysbiosis oral graft-versus-host disease oral mucositis conditioning |
| url | https://www.mdpi.com/2076-2607/10/4/734 |
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