A Comparative Prospective Study in Evaluating <i>Candida</i> spp. In Vitro Susceptibility through Micronaut-AM and Sensititre Yeast-One
Background. Among invasive fungal infection pathogens, <i>Candida</i> spp. represent the most common aetiological agents. The increasing rate of severe infections and the emergence of antimicrobial resistance highlight the importance of in vitro susceptibility testing. The EUCAST and the...
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MDPI AG
2023-08-01
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author | Laura Trovato Maddalena Calvo Guido Scalia Salvatore Oliveri |
author_facet | Laura Trovato Maddalena Calvo Guido Scalia Salvatore Oliveri |
author_sort | Laura Trovato |
collection | DOAJ |
description | Background. Among invasive fungal infection pathogens, <i>Candida</i> spp. represent the most common aetiological agents. The increasing rate of severe infections and the emergence of antimicrobial resistance highlight the importance of in vitro susceptibility testing. The EUCAST and the CLSI have established reference microdilutions that are reliable but difficult to apply in a laboratory routine. Commercial microdilutions could represent a valuable alternative within a diagnostic workflow. Methods. A number of 50 <i>Candida</i> spp. collected from positive blood samples simultaneously underwent the Sensititre Yeast-One microdilution as a standard susceptibility test and the Micronaut-AM as an experimental method. A comparison between the two techniques was produced, evaluating the effectiveness of the Micronaut-AM compared to the extensively consolidated Sensititre Yeast-One. Results. The two techniques revealed optimal agreement rates, confirming the reliability of the commercial microdilution kits within the diagnostic workflows. The results showed remarkable concordance for both susceptible and resistant isolates, highlighting slight variations in the different identified <i>Candida</i> species. Conclusions. Future studies about antifungal susceptibility testing should be encouraged, including molecular confirmation of possible resistance phenotypes and extended isolate numbers for the different <i>Candida</i> species. Moreover, it would be interesting to plan clinical trials after the execution of the examined commercial microdilution methods. |
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spelling | doaj.art-54f1c9d89f224a90bc3819e8f91fc53e2023-11-19T11:58:18ZengMDPI AGMicrobiology Research2036-74812023-08-011431077108810.3390/microbiolres14030072A Comparative Prospective Study in Evaluating <i>Candida</i> spp. In Vitro Susceptibility through Micronaut-AM and Sensititre Yeast-OneLaura Trovato0Maddalena Calvo1Guido Scalia2Salvatore Oliveri3Department of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, ItalyDepartment of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, ItalyBackground. Among invasive fungal infection pathogens, <i>Candida</i> spp. represent the most common aetiological agents. The increasing rate of severe infections and the emergence of antimicrobial resistance highlight the importance of in vitro susceptibility testing. The EUCAST and the CLSI have established reference microdilutions that are reliable but difficult to apply in a laboratory routine. Commercial microdilutions could represent a valuable alternative within a diagnostic workflow. Methods. A number of 50 <i>Candida</i> spp. collected from positive blood samples simultaneously underwent the Sensititre Yeast-One microdilution as a standard susceptibility test and the Micronaut-AM as an experimental method. A comparison between the two techniques was produced, evaluating the effectiveness of the Micronaut-AM compared to the extensively consolidated Sensititre Yeast-One. Results. The two techniques revealed optimal agreement rates, confirming the reliability of the commercial microdilution kits within the diagnostic workflows. The results showed remarkable concordance for both susceptible and resistant isolates, highlighting slight variations in the different identified <i>Candida</i> species. Conclusions. Future studies about antifungal susceptibility testing should be encouraged, including molecular confirmation of possible resistance phenotypes and extended isolate numbers for the different <i>Candida</i> species. Moreover, it would be interesting to plan clinical trials after the execution of the examined commercial microdilution methods.https://www.mdpi.com/2036-7481/14/3/72antifungal susceptibilitybroth microdilution<i>Candida</i> spp.Micronaut-AM |
spellingShingle | Laura Trovato Maddalena Calvo Guido Scalia Salvatore Oliveri A Comparative Prospective Study in Evaluating <i>Candida</i> spp. In Vitro Susceptibility through Micronaut-AM and Sensititre Yeast-One Microbiology Research antifungal susceptibility broth microdilution <i>Candida</i> spp. Micronaut-AM |
title | A Comparative Prospective Study in Evaluating <i>Candida</i> spp. In Vitro Susceptibility through Micronaut-AM and Sensititre Yeast-One |
title_full | A Comparative Prospective Study in Evaluating <i>Candida</i> spp. In Vitro Susceptibility through Micronaut-AM and Sensititre Yeast-One |
title_fullStr | A Comparative Prospective Study in Evaluating <i>Candida</i> spp. In Vitro Susceptibility through Micronaut-AM and Sensititre Yeast-One |
title_full_unstemmed | A Comparative Prospective Study in Evaluating <i>Candida</i> spp. In Vitro Susceptibility through Micronaut-AM and Sensititre Yeast-One |
title_short | A Comparative Prospective Study in Evaluating <i>Candida</i> spp. In Vitro Susceptibility through Micronaut-AM and Sensititre Yeast-One |
title_sort | comparative prospective study in evaluating i candida i spp in vitro susceptibility through micronaut am and sensititre yeast one |
topic | antifungal susceptibility broth microdilution <i>Candida</i> spp. Micronaut-AM |
url | https://www.mdpi.com/2036-7481/14/3/72 |
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