Protective mechanism of agmatine pretreatment on RGC-5 cells injured by oxidative stress
Agmatine has neuroprotective effects on retinal ganglion cells (RGCs) as well as cortical and spinal neurons. It protects RGCs from oxidative stress even when it is not present at the time of injury. As agmatine has high affinity for various cellular receptors, we assessed protective mechanisms of a...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Associação Brasileira de Divulgação Científica
2010-04-01
|
| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400006 |
| _version_ | 1818298025874817024 |
|---|---|
| author | Y. Iizuka S. Hong C.Y. Kim W.I. Yang J.E. Lee G.J. Seong |
| author_facet | Y. Iizuka S. Hong C.Y. Kim W.I. Yang J.E. Lee G.J. Seong |
| author_sort | Y. Iizuka |
| collection | DOAJ |
| description | Agmatine has neuroprotective effects on retinal ganglion cells (RGCs) as well as cortical and spinal neurons. It protects RGCs from oxidative stress even when it is not present at the time of injury. As agmatine has high affinity for various cellular receptors, we assessed protective mechanisms of agmatine using transformed RGCs (RGC-5 cell line). Differentiated RGC-5 cells were pretreated with 100 μM agmatine and consecutively exposed to 1.0 mM hydrogen peroxide (H2O2). Cell viability was determined by measuring lactate dehydrogenase (LDH), and the effects of selective alpha 2-adrenergic receptor antagonist yohimbine (0-500 nM) and N-methyl-D-aspartic acid (NMDA) receptor agonist NMDA (0-100 µM) were evaluated. Agmatine’s protective effect was compared to a selective NMDA receptor antagonist MK-801. After a 16-h exposure to H2O2, the LDH assay showed cell loss greater than 50%, which was reduced to about 30% when agmatine was pretreated before injury. Yohimbine almost completely inhibited agmatine’s protective effect, but NMDA did not. In addition, MK-801 (0-100 µM) did not significantly attenuate the H2O2-induced cytotoxicity. Our results suggest that neuroprotective effects of agmatine on RGCs under oxidative stress may be mainly attributed to the alpha 2-adrenergic receptor signaling pathway. |
| first_indexed | 2024-12-13T04:28:46Z |
| format | Article |
| id | doaj.art-54f1f916d68941fc802aea365a4eb4b4 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-13T04:28:46Z |
| publishDate | 2010-04-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-54f1f916d68941fc802aea365a4eb4b42022-12-21T23:59:37ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2010-04-01434356358Protective mechanism of agmatine pretreatment on RGC-5 cells injured by oxidative stressY. IizukaS. HongC.Y. KimW.I. YangJ.E. LeeG.J. SeongAgmatine has neuroprotective effects on retinal ganglion cells (RGCs) as well as cortical and spinal neurons. It protects RGCs from oxidative stress even when it is not present at the time of injury. As agmatine has high affinity for various cellular receptors, we assessed protective mechanisms of agmatine using transformed RGCs (RGC-5 cell line). Differentiated RGC-5 cells were pretreated with 100 μM agmatine and consecutively exposed to 1.0 mM hydrogen peroxide (H2O2). Cell viability was determined by measuring lactate dehydrogenase (LDH), and the effects of selective alpha 2-adrenergic receptor antagonist yohimbine (0-500 nM) and N-methyl-D-aspartic acid (NMDA) receptor agonist NMDA (0-100 µM) were evaluated. Agmatine’s protective effect was compared to a selective NMDA receptor antagonist MK-801. After a 16-h exposure to H2O2, the LDH assay showed cell loss greater than 50%, which was reduced to about 30% when agmatine was pretreated before injury. Yohimbine almost completely inhibited agmatine’s protective effect, but NMDA did not. In addition, MK-801 (0-100 µM) did not significantly attenuate the H2O2-induced cytotoxicity. Our results suggest that neuroprotective effects of agmatine on RGCs under oxidative stress may be mainly attributed to the alpha 2-adrenergic receptor signaling pathway.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400006AgmatineNeuroprotectionOxidative stressRetinal ganglion cell |
| spellingShingle | Y. Iizuka S. Hong C.Y. Kim W.I. Yang J.E. Lee G.J. Seong Protective mechanism of agmatine pretreatment on RGC-5 cells injured by oxidative stress Brazilian Journal of Medical and Biological Research Agmatine Neuroprotection Oxidative stress Retinal ganglion cell |
| title | Protective mechanism of agmatine pretreatment on RGC-5 cells injured by oxidative stress |
| title_full | Protective mechanism of agmatine pretreatment on RGC-5 cells injured by oxidative stress |
| title_fullStr | Protective mechanism of agmatine pretreatment on RGC-5 cells injured by oxidative stress |
| title_full_unstemmed | Protective mechanism of agmatine pretreatment on RGC-5 cells injured by oxidative stress |
| title_short | Protective mechanism of agmatine pretreatment on RGC-5 cells injured by oxidative stress |
| title_sort | protective mechanism of agmatine pretreatment on rgc 5 cells injured by oxidative stress |
| topic | Agmatine Neuroprotection Oxidative stress Retinal ganglion cell |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000400006 |
| work_keys_str_mv | AT yiizuka protectivemechanismofagmatinepretreatmentonrgc5cellsinjuredbyoxidativestress AT shong protectivemechanismofagmatinepretreatmentonrgc5cellsinjuredbyoxidativestress AT cykim protectivemechanismofagmatinepretreatmentonrgc5cellsinjuredbyoxidativestress AT wiyang protectivemechanismofagmatinepretreatmentonrgc5cellsinjuredbyoxidativestress AT jelee protectivemechanismofagmatinepretreatmentonrgc5cellsinjuredbyoxidativestress AT gjseong protectivemechanismofagmatinepretreatmentonrgc5cellsinjuredbyoxidativestress |