Biotechnological Production of Fumaric Acid by <i>Rhizopus arrhizus</i>—Reaching Industrially Relevant Final Titers
Fumaric acid is used in various areas of the chemical industry due to its functional groups. For example, it is used in the polymer industry to produce unsaturated polyester resins, which are nowadays mostly produced from fossil raw materials. With regard to sustainable biotechnological fumaric acid...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/7/588 |
| _version_ | 1797589365754429440 |
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| author | Anja Kuenz Laslo Eidt Ulf Prüße |
| author_facet | Anja Kuenz Laslo Eidt Ulf Prüße |
| author_sort | Anja Kuenz |
| collection | DOAJ |
| description | Fumaric acid is used in various areas of the chemical industry due to its functional groups. For example, it is used in the polymer industry to produce unsaturated polyester resins, which are nowadays mostly produced from fossil raw materials. With regard to sustainable biotechnological fumaric acid production, the main challenge is to develop a cost-effective and robust fermentation process with industrially relevant final titers, productivities and yields. For biotechnological fumaric acid production, mainly fungi of the genus <i>Rhizopus</i> are used, which require very complex and challenging morphology control. The aim of this work is the development of an effective biotechnological fumaric acid production process with <i>R. arrhizus</i> NRRL 1526. Significant insights into the morphology control of the fungus and optimization of production characteristics were obtained, and a final titer of 86.3 g/L fumaric acid was achieved in a batch cultivation, with a yield of 0.67 g/g and a productivity of 0.60 g/(L∙h). In addition, a fed-batch process was developed, in which the production phase was extended, and a maximum final titer of 195.4 g/L fumaric acid was achieved. According to current knowledge, this value is the highest final concentration of fumaric acid produced using biotechnology. |
| first_indexed | 2024-03-11T01:05:36Z |
| format | Article |
| id | doaj.art-54f33c120b7e444483e3f17afe54539e |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T01:05:36Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-54f33c120b7e444483e3f17afe54539e2023-11-18T19:15:31ZengMDPI AGFermentation2311-56372023-06-019758810.3390/fermentation9070588Biotechnological Production of Fumaric Acid by <i>Rhizopus arrhizus</i>—Reaching Industrially Relevant Final TitersAnja Kuenz0Laslo Eidt1Ulf Prüße2Thünen Institute of Agricultural Technology, 38116 Braunschweig, GermanyThünen Institute of Agricultural Technology, 38116 Braunschweig, GermanyThünen Institute of Agricultural Technology, 38116 Braunschweig, GermanyFumaric acid is used in various areas of the chemical industry due to its functional groups. For example, it is used in the polymer industry to produce unsaturated polyester resins, which are nowadays mostly produced from fossil raw materials. With regard to sustainable biotechnological fumaric acid production, the main challenge is to develop a cost-effective and robust fermentation process with industrially relevant final titers, productivities and yields. For biotechnological fumaric acid production, mainly fungi of the genus <i>Rhizopus</i> are used, which require very complex and challenging morphology control. The aim of this work is the development of an effective biotechnological fumaric acid production process with <i>R. arrhizus</i> NRRL 1526. Significant insights into the morphology control of the fungus and optimization of production characteristics were obtained, and a final titer of 86.3 g/L fumaric acid was achieved in a batch cultivation, with a yield of 0.67 g/g and a productivity of 0.60 g/(L∙h). In addition, a fed-batch process was developed, in which the production phase was extended, and a maximum final titer of 195.4 g/L fumaric acid was achieved. According to current knowledge, this value is the highest final concentration of fumaric acid produced using biotechnology.https://www.mdpi.com/2311-5637/9/7/588fumaric acid<i>Rhizopus arrhizus</i>process optimizationfermentation |
| spellingShingle | Anja Kuenz Laslo Eidt Ulf Prüße Biotechnological Production of Fumaric Acid by <i>Rhizopus arrhizus</i>—Reaching Industrially Relevant Final Titers Fermentation fumaric acid <i>Rhizopus arrhizus</i> process optimization fermentation |
| title | Biotechnological Production of Fumaric Acid by <i>Rhizopus arrhizus</i>—Reaching Industrially Relevant Final Titers |
| title_full | Biotechnological Production of Fumaric Acid by <i>Rhizopus arrhizus</i>—Reaching Industrially Relevant Final Titers |
| title_fullStr | Biotechnological Production of Fumaric Acid by <i>Rhizopus arrhizus</i>—Reaching Industrially Relevant Final Titers |
| title_full_unstemmed | Biotechnological Production of Fumaric Acid by <i>Rhizopus arrhizus</i>—Reaching Industrially Relevant Final Titers |
| title_short | Biotechnological Production of Fumaric Acid by <i>Rhizopus arrhizus</i>—Reaching Industrially Relevant Final Titers |
| title_sort | biotechnological production of fumaric acid by i rhizopus arrhizus i reaching industrially relevant final titers |
| topic | fumaric acid <i>Rhizopus arrhizus</i> process optimization fermentation |
| url | https://www.mdpi.com/2311-5637/9/7/588 |
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