Cold Stress Response Genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Lantiplantibacillus plantarum subsp. plantarum Mut-7 Support the Ability to Survive in Low-Temperature Conditions
Probiotics are widely consumed in various food matrices to provide health benefits to the host. The viability of probiotic cells is influenced by several factors, including exposure to high temperatures during the production process and low temperatures during storage. In this study, we report the...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2022-08-01
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| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/39679 |
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| author | Eunike Marganingrum Andriani Samodra Dian Suroto Tyas Utami Rachma Wikandari Endang Sutriswati Rahayu |
| author_facet | Eunike Marganingrum Andriani Samodra Dian Suroto Tyas Utami Rachma Wikandari Endang Sutriswati Rahayu |
| author_sort | Eunike Marganingrum Andriani Samodra |
| collection | DOAJ |
| description |
Probiotics are widely consumed in various food matrices to provide health benefits to the host. The viability of probiotic cells is influenced by several factors, including exposure to high temperatures during the production process and low temperatures during storage. In this study, we report the response to cold stress of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 after 24 h of storage at 4°C and -20℃. The cell number of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 in low-temperature condition had no significant differences than their initial number: 11.88 log CFU/ml and 11.62 log CFU/ml at 4°C; 11.51 log CFU/ml and 11.47 log CFU/ml at -20°C for Mut-3 and Mut-7 respectively. The results indicated the survival capacity of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 at low temperatures. The genes encoding cold shock proteins for the response to cold stress were evaluated by genome sequencing. The CspA/CspC genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 possibly play a role in maintaining cell resistance at low temperatures, since the genes products predicted to have conserved motifs in the RNA binding protein (RNP) -1 and RNP-2 responsible for cold response stress which are similar to those in other bacteria.
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| first_indexed | 2024-04-11T13:49:49Z |
| format | Article |
| id | doaj.art-54f3f7c590504a428bc3f04e469faf4f |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-04-11T13:49:49Z |
| publishDate | 2022-08-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-54f3f7c590504a428bc3f04e469faf4f2022-12-22T04:20:43ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942022-08-0130110.4308/hjb.30.1.65-70Cold Stress Response Genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Lantiplantibacillus plantarum subsp. plantarum Mut-7 Support the Ability to Survive in Low-Temperature ConditionsEunike Marganingrum Andriani Samodra0Dian Suroto1Tyas Utami2Rachma Wikandari3Endang Sutriswati Rahayu4Faculty of Agricultural Technology, Universitas Gadjah Mada, Flora Street Number 1 Bulaksumur, Yogyakarta, IndonesiaFaculty of Agricultural Technology, Universitas Gadjah Mada, Flora Street Number 1 Bulaksumur, Yogyakarta, Indonesia. Center for Food and Nutrition Studies, Universitas Gadjah Mada, Teknika Utara Street , Yogyakarta, Indonesia. University Center of Excellence for Research and Application on Integrated Probiotic Industry, Universitas Gadjah Mada, Yogyakarta, IndonesiaFaculty of Agricultural Technology, Universitas Gadjah Mada, Flora Street Number 1 Bulaksumur, Yogyakarta, Indonesia. Center for Food and Nutrition Studies, Universitas Gadjah Mada, Teknika Utara Street , Yogyakarta, Indonesia. University Center of Excellence for Research and Application on Integrated Probiotic Industry, Universitas Gadjah Mada, Yogyakarta, IndonesiaFaculty of Agricultural Technology, Universitas Gadjah Mada, Flora Street Number 1 Bulaksumur, Yogyakarta, Indonesia. Center for Food and Nutrition Studies, Universitas Gadjah Mada, Teknika Utara Street , Yogyakarta, IndonesiaFaculty of Agricultural Technology, Universitas Gadjah Mada, Flora Street Number 1 Bulaksumur, Yogyakarta, Indonesia. Center for Food and Nutrition Studies, Universitas Gadjah Mada, Teknika Utara Street , Yogyakarta, Indonesia. University Center of Excellence for Research and Application on Integrated Probiotic Industry, Universitas Gadjah Mada, Yogyakarta, Indonesia Probiotics are widely consumed in various food matrices to provide health benefits to the host. The viability of probiotic cells is influenced by several factors, including exposure to high temperatures during the production process and low temperatures during storage. In this study, we report the response to cold stress of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 after 24 h of storage at 4°C and -20℃. The cell number of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 in low-temperature condition had no significant differences than their initial number: 11.88 log CFU/ml and 11.62 log CFU/ml at 4°C; 11.51 log CFU/ml and 11.47 log CFU/ml at -20°C for Mut-3 and Mut-7 respectively. The results indicated the survival capacity of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 at low temperatures. The genes encoding cold shock proteins for the response to cold stress were evaluated by genome sequencing. The CspA/CspC genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 possibly play a role in maintaining cell resistance at low temperatures, since the genes products predicted to have conserved motifs in the RNA binding protein (RNP) -1 and RNP-2 responsible for cold response stress which are similar to those in other bacteria. https://journal.ipb.ac.id/index.php/hayati/article/view/39679 |
| spellingShingle | Eunike Marganingrum Andriani Samodra Dian Suroto Tyas Utami Rachma Wikandari Endang Sutriswati Rahayu Cold Stress Response Genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Lantiplantibacillus plantarum subsp. plantarum Mut-7 Support the Ability to Survive in Low-Temperature Conditions Hayati Journal of Biosciences |
| title | Cold Stress Response Genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Lantiplantibacillus plantarum subsp. plantarum Mut-7 Support the Ability to Survive in Low-Temperature Conditions |
| title_full | Cold Stress Response Genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Lantiplantibacillus plantarum subsp. plantarum Mut-7 Support the Ability to Survive in Low-Temperature Conditions |
| title_fullStr | Cold Stress Response Genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Lantiplantibacillus plantarum subsp. plantarum Mut-7 Support the Ability to Survive in Low-Temperature Conditions |
| title_full_unstemmed | Cold Stress Response Genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Lantiplantibacillus plantarum subsp. plantarum Mut-7 Support the Ability to Survive in Low-Temperature Conditions |
| title_short | Cold Stress Response Genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Lantiplantibacillus plantarum subsp. plantarum Mut-7 Support the Ability to Survive in Low-Temperature Conditions |
| title_sort | cold stress response genes of lantiplantibacillus plantarum subsp plantarum mut 3 and lantiplantibacillus plantarum subsp plantarum mut 7 support the ability to survive in low temperature conditions |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/39679 |
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