Hsa_circ_0005397 promotes hepatocellular carcinoma progression through EIF4A3
Abstract Purpose The purpose of this study was to explore the expression and potential mechanism of hsa_circ_0005397 in hepatocellular carcinoma progression. Methods Quantitative reverse transcription-polymerase chain reaction(qRT-PCR) was used to measure the expression level of hsa_circ_0005397 and...
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| Format: | Article |
| Language: | English |
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BMC
2024-02-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-024-11984-6 |
| _version_ | 1797274297369100288 |
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| author | Liu-Xia Yuan Mei Luo Ruo-Yu Liu Hui-Xuan Wang Lin-Ling Ju Feng Wang Ya-Li Cao Zhong-Cheng Wang Lin Chen |
| author_facet | Liu-Xia Yuan Mei Luo Ruo-Yu Liu Hui-Xuan Wang Lin-Ling Ju Feng Wang Ya-Li Cao Zhong-Cheng Wang Lin Chen |
| author_sort | Liu-Xia Yuan |
| collection | DOAJ |
| description | Abstract Purpose The purpose of this study was to explore the expression and potential mechanism of hsa_circ_0005397 in hepatocellular carcinoma progression. Methods Quantitative reverse transcription-polymerase chain reaction(qRT-PCR) was used to measure the expression level of hsa_circ_0005397 and EIF4A3 from paired HCC tissues and cell lines. Western Blot (WB) and immunohistochemistry (IHC) were used to verify the protein level of EIF4A3. The specificity of primers was confirmed by agarose gel electrophoresis. Receiver Operating Characteristic (ROC) Curve was drawn to analyze diagnostic value. Actinomycin D and nuclear and cytoplasmic extraction assays were utilized to evaluate the characteristics of hsa_circ_0005397. Cell Counting kit-8 (CCK-8) and colony formation assays were performed to detect cell proliferation. Flow cytometry analysis was used to detect the cell cycle. Transwell assay was performed to determine migration and invasion ability. RNA-binding proteins (RBPs) of hsa_circ_0005397 in HCC were explored using bioinformatics websites. The relationship between hsa_circ_0005397 and Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) was verified by RNA Binding Protein Immunoprecipitation (RIP) assays, correlation and rescue experiments. Results In this study, hsa_circ_0005397 was found to be significantly upregulated in HCC, and the good diagnostic sensitivity and specificity shown a potential diagnostic capability. Upregulated expression of hsa_circ_0005397 was significantly related to tumor size and stage. Hsa_circ_0005397 was circular structure which more stable than liner mRNA, and mostly distributed in the cytoplasm. Upregulation of hsa_circ_0005397 generally resulted in stronger proliferative ability, clonality, and metastatic potency of HCC cells; its downregulation yielded the opposite results. EIF4A3 is an RNA-binding protein of hsa_circ_0005397, which overexpressed in paired HCC tissues and cell lines. In addition, expression of hsa_circ_0005397 decreased equally when EIF4A3 was depleted. RIP assays and correlation assay estimated that EIF4A3 could interacted with hsa_circ_0005397. Knockdown of EIF4A3 could reverse hsa_circ_0005397 function in HCC progression. Conclusions Hsa_circ_0005397 promotes progression of hepatocellular carcinoma through EIF4A3. These research findings may provide novel clinical value for hepatocellular carcinoma. |
| first_indexed | 2024-03-07T14:56:23Z |
| format | Article |
| id | doaj.art-54f5049d67ea4ae6a45dc06455a9aa17 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-03-07T14:56:23Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-54f5049d67ea4ae6a45dc06455a9aa172024-03-05T19:22:45ZengBMCBMC Cancer1471-24072024-02-0124111110.1186/s12885-024-11984-6Hsa_circ_0005397 promotes hepatocellular carcinoma progression through EIF4A3Liu-Xia Yuan0Mei Luo1Ruo-Yu Liu2Hui-Xuan Wang3Lin-Ling Ju4Feng Wang5Ya-Li Cao6Zhong-Cheng Wang7Lin Chen8Institute of Liver Diseases, Nantong Third People’s Hospital, Affiliated Nantong Hospital 3 of Nantong UniversityNantong Third People’s Hospital, Medical School of Nantong UniversityMedical School of Nantong University, Affiliated Hospital of Nantong UniversityInstitute of Liver Diseases, Nantong Third People’s Hospital, Affiliated Nantong Hospital 3 of Nantong UniversityInstitute of Liver Diseases, Nantong Third People’s Hospital, Affiliated Nantong Hospital 3 of Nantong UniversityMedical School of Nantong University, Affiliated Hospital of Nantong UniversityPreventive Health Department, Nantong Third People’s Hospital, Affiliated Nantong Hospital 3 of Nantong UniversityHepatology Department of integrated Chinese and Western Medicine, Nantong Third People’s Hospital, Affiliated Nantong Hospital 3 of Nantong UniversityInstitute of Liver Diseases, Nantong Third People’s Hospital, Affiliated Nantong Hospital 3 of Nantong UniversityAbstract Purpose The purpose of this study was to explore the expression and potential mechanism of hsa_circ_0005397 in hepatocellular carcinoma progression. Methods Quantitative reverse transcription-polymerase chain reaction(qRT-PCR) was used to measure the expression level of hsa_circ_0005397 and EIF4A3 from paired HCC tissues and cell lines. Western Blot (WB) and immunohistochemistry (IHC) were used to verify the protein level of EIF4A3. The specificity of primers was confirmed by agarose gel electrophoresis. Receiver Operating Characteristic (ROC) Curve was drawn to analyze diagnostic value. Actinomycin D and nuclear and cytoplasmic extraction assays were utilized to evaluate the characteristics of hsa_circ_0005397. Cell Counting kit-8 (CCK-8) and colony formation assays were performed to detect cell proliferation. Flow cytometry analysis was used to detect the cell cycle. Transwell assay was performed to determine migration and invasion ability. RNA-binding proteins (RBPs) of hsa_circ_0005397 in HCC were explored using bioinformatics websites. The relationship between hsa_circ_0005397 and Eukaryotic Translation Initiation Factor 4A3 (EIF4A3) was verified by RNA Binding Protein Immunoprecipitation (RIP) assays, correlation and rescue experiments. Results In this study, hsa_circ_0005397 was found to be significantly upregulated in HCC, and the good diagnostic sensitivity and specificity shown a potential diagnostic capability. Upregulated expression of hsa_circ_0005397 was significantly related to tumor size and stage. Hsa_circ_0005397 was circular structure which more stable than liner mRNA, and mostly distributed in the cytoplasm. Upregulation of hsa_circ_0005397 generally resulted in stronger proliferative ability, clonality, and metastatic potency of HCC cells; its downregulation yielded the opposite results. EIF4A3 is an RNA-binding protein of hsa_circ_0005397, which overexpressed in paired HCC tissues and cell lines. In addition, expression of hsa_circ_0005397 decreased equally when EIF4A3 was depleted. RIP assays and correlation assay estimated that EIF4A3 could interacted with hsa_circ_0005397. Knockdown of EIF4A3 could reverse hsa_circ_0005397 function in HCC progression. Conclusions Hsa_circ_0005397 promotes progression of hepatocellular carcinoma through EIF4A3. These research findings may provide novel clinical value for hepatocellular carcinoma.https://doi.org/10.1186/s12885-024-11984-6Hepatocellular carcinomahsa_circ_0005397EIF4A3 |
| spellingShingle | Liu-Xia Yuan Mei Luo Ruo-Yu Liu Hui-Xuan Wang Lin-Ling Ju Feng Wang Ya-Li Cao Zhong-Cheng Wang Lin Chen Hsa_circ_0005397 promotes hepatocellular carcinoma progression through EIF4A3 BMC Cancer Hepatocellular carcinoma hsa_circ_0005397 EIF4A3 |
| title | Hsa_circ_0005397 promotes hepatocellular carcinoma progression through EIF4A3 |
| title_full | Hsa_circ_0005397 promotes hepatocellular carcinoma progression through EIF4A3 |
| title_fullStr | Hsa_circ_0005397 promotes hepatocellular carcinoma progression through EIF4A3 |
| title_full_unstemmed | Hsa_circ_0005397 promotes hepatocellular carcinoma progression through EIF4A3 |
| title_short | Hsa_circ_0005397 promotes hepatocellular carcinoma progression through EIF4A3 |
| title_sort | hsa circ 0005397 promotes hepatocellular carcinoma progression through eif4a3 |
| topic | Hepatocellular carcinoma hsa_circ_0005397 EIF4A3 |
| url | https://doi.org/10.1186/s12885-024-11984-6 |
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