Single Nucleotide Polymorphisms Associated with Metformin and Sulphonylureas’ Glycaemic Response among South African Adults with Type 2 Diabetes Mellitus
Aims: To examine the association of polymorphisms belonging to <i>SLC22A1</i>, <i>SP1</i>, <i>PRPF31</i>, <i>NBEA</i>, <i>SCNN1B</i>, <i>CPA6</i> and <i>CAPN10</i> genes with glycaemic response to metformin and sulph...
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| Format: | Article |
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MDPI AG
2021-02-01
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| Series: | Journal of Personalized Medicine |
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| Online Access: | https://www.mdpi.com/2075-4426/11/2/104 |
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| author | Charity Masilela Brendon Pearce Joven Jebio Ongole Oladele Vincent Adeniyi Mongi Benjeddou |
| author_facet | Charity Masilela Brendon Pearce Joven Jebio Ongole Oladele Vincent Adeniyi Mongi Benjeddou |
| author_sort | Charity Masilela |
| collection | DOAJ |
| description | Aims: To examine the association of polymorphisms belonging to <i>SLC22A1</i>, <i>SP1</i>, <i>PRPF31</i>, <i>NBEA</i>, <i>SCNN1B</i>, <i>CPA6</i> and <i>CAPN10</i> genes with glycaemic response to metformin and sulphonylureas (SU) combination therapy among South African adults with diabetes mellitus type 2 (T2DM). Methods: A total of 128 individuals of Swati (<i>n</i> = 22) and Zulu (<i>n</i> = 106) origin attending chronic care for T2DM were recruited. Nine SNPs previously associated with metformin and SUs were selected and genotyped using MassArray. Uncontrolled T2DM was defined as HbA1c > 7%. The association between genotypes, alleles and glycaemic response to treatment was determined using multivariate logistic regression model analysis. Results: About 85.93% (<i>n</i> = 110) of the study participants were female and 77.34% (<i>n</i> = 99) had uncontrolled T2DM (HbA1c > 7%). In the multivariate (adjusted) logistic regression model analysis, the CC genotype of rs2162145 (<i>CPA6</i>), GG and GA genotypes of rs889299 (<i>SCNN1B</i>) were significantly associated with uncontrolled T2DM. On the other hand, the C allele of rs254271 (<i>PRPF31</i>) and the GA genotype of rs3792269 (<i>CAPN10</i>) were associated with controlled T2DM. A significant interaction between rs2162145 and rs889299 in response to metformin and SU combination therapy was observed. Conclusions: In this study, we reported the association of rs2162145 (CC) and rs889299 (GG and GA) with uncontrolled T2DM. We also reported the association of rs254271 (C) and rs3792269 (GA) with controlled T2DM in response to metformin and SU combination therapy. Furthermore, an interaction between rs2162145 and rs889299 was established, where the genotype combination GA (rs889299) and TT (rs2162145) was associated with uncontrolled T2DM. |
| first_indexed | 2024-03-09T05:23:10Z |
| format | Article |
| id | doaj.art-54f831347f7943cea424b3fece49cb2a |
| institution | Directory Open Access Journal |
| issn | 2075-4426 |
| language | English |
| last_indexed | 2024-03-09T05:23:10Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Personalized Medicine |
| spelling | doaj.art-54f831347f7943cea424b3fece49cb2a2023-12-03T12:38:43ZengMDPI AGJournal of Personalized Medicine2075-44262021-02-0111210410.3390/jpm11020104Single Nucleotide Polymorphisms Associated with Metformin and Sulphonylureas’ Glycaemic Response among South African Adults with Type 2 Diabetes MellitusCharity Masilela0Brendon Pearce1Joven Jebio Ongole2Oladele Vincent Adeniyi3Mongi Benjeddou4Department of Biotechnology, University of the Western Cape, Bellville 7535, South AfricaDepartment of Biotechnology, University of the Western Cape, Bellville 7535, South AfricaCenter for Teaching and Learning, Department of Family Medicine, Piet Retief Hospital, Mkhondo 2380, South AfricaDepartment of Family Medicine, Walter Sisulu University, East London 5200, South AfricaDepartment of Biotechnology, University of the Western Cape, Bellville 7535, South AfricaAims: To examine the association of polymorphisms belonging to <i>SLC22A1</i>, <i>SP1</i>, <i>PRPF31</i>, <i>NBEA</i>, <i>SCNN1B</i>, <i>CPA6</i> and <i>CAPN10</i> genes with glycaemic response to metformin and sulphonylureas (SU) combination therapy among South African adults with diabetes mellitus type 2 (T2DM). Methods: A total of 128 individuals of Swati (<i>n</i> = 22) and Zulu (<i>n</i> = 106) origin attending chronic care for T2DM were recruited. Nine SNPs previously associated with metformin and SUs were selected and genotyped using MassArray. Uncontrolled T2DM was defined as HbA1c > 7%. The association between genotypes, alleles and glycaemic response to treatment was determined using multivariate logistic regression model analysis. Results: About 85.93% (<i>n</i> = 110) of the study participants were female and 77.34% (<i>n</i> = 99) had uncontrolled T2DM (HbA1c > 7%). In the multivariate (adjusted) logistic regression model analysis, the CC genotype of rs2162145 (<i>CPA6</i>), GG and GA genotypes of rs889299 (<i>SCNN1B</i>) were significantly associated with uncontrolled T2DM. On the other hand, the C allele of rs254271 (<i>PRPF31</i>) and the GA genotype of rs3792269 (<i>CAPN10</i>) were associated with controlled T2DM. A significant interaction between rs2162145 and rs889299 in response to metformin and SU combination therapy was observed. Conclusions: In this study, we reported the association of rs2162145 (CC) and rs889299 (GG and GA) with uncontrolled T2DM. We also reported the association of rs254271 (C) and rs3792269 (GA) with controlled T2DM in response to metformin and SU combination therapy. Furthermore, an interaction between rs2162145 and rs889299 was established, where the genotype combination GA (rs889299) and TT (rs2162145) was associated with uncontrolled T2DM.https://www.mdpi.com/2075-4426/11/2/104type 2 diabetessingle nucleotide polymorphismsmetforminsulphonylureascombination therapy |
| spellingShingle | Charity Masilela Brendon Pearce Joven Jebio Ongole Oladele Vincent Adeniyi Mongi Benjeddou Single Nucleotide Polymorphisms Associated with Metformin and Sulphonylureas’ Glycaemic Response among South African Adults with Type 2 Diabetes Mellitus Journal of Personalized Medicine type 2 diabetes single nucleotide polymorphisms metformin sulphonylureas combination therapy |
| title | Single Nucleotide Polymorphisms Associated with Metformin and Sulphonylureas’ Glycaemic Response among South African Adults with Type 2 Diabetes Mellitus |
| title_full | Single Nucleotide Polymorphisms Associated with Metformin and Sulphonylureas’ Glycaemic Response among South African Adults with Type 2 Diabetes Mellitus |
| title_fullStr | Single Nucleotide Polymorphisms Associated with Metformin and Sulphonylureas’ Glycaemic Response among South African Adults with Type 2 Diabetes Mellitus |
| title_full_unstemmed | Single Nucleotide Polymorphisms Associated with Metformin and Sulphonylureas’ Glycaemic Response among South African Adults with Type 2 Diabetes Mellitus |
| title_short | Single Nucleotide Polymorphisms Associated with Metformin and Sulphonylureas’ Glycaemic Response among South African Adults with Type 2 Diabetes Mellitus |
| title_sort | single nucleotide polymorphisms associated with metformin and sulphonylureas glycaemic response among south african adults with type 2 diabetes mellitus |
| topic | type 2 diabetes single nucleotide polymorphisms metformin sulphonylureas combination therapy |
| url | https://www.mdpi.com/2075-4426/11/2/104 |
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