Local evolution of seed flotation in Arabidopsis.
Arabidopsis seeds rapidly release hydrophilic polysaccharides from the seed coat on imbibition. These form a heavy mucilage layer around the seed that makes it sink in water. Fourteen natural Arabidopsis variants from central Asia and Scandinavia were identified with seeds that have modified mucilag...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-03-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC3953066?pdf=render |
| _version_ | 1811232653479772160 |
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| author | Susana Saez-Aguayo Corinne Rondeau-Mouro Audrey Macquet Ilkka Kronholm Marie-Christine Ralet Adeline Berger Christine Sallé Damien Poulain Fabienne Granier Lucy Botran Olivier Loudet Juliette de Meaux Annie Marion-Poll Helen M North |
| author_facet | Susana Saez-Aguayo Corinne Rondeau-Mouro Audrey Macquet Ilkka Kronholm Marie-Christine Ralet Adeline Berger Christine Sallé Damien Poulain Fabienne Granier Lucy Botran Olivier Loudet Juliette de Meaux Annie Marion-Poll Helen M North |
| author_sort | Susana Saez-Aguayo |
| collection | DOAJ |
| description | Arabidopsis seeds rapidly release hydrophilic polysaccharides from the seed coat on imbibition. These form a heavy mucilage layer around the seed that makes it sink in water. Fourteen natural Arabidopsis variants from central Asia and Scandinavia were identified with seeds that have modified mucilage release and float. Four of these have a novel mucilage phenotype with almost none of the released mucilage adhering to the seed and the absence of cellulose microfibrils. Mucilage release was modified in the variants by ten independent causal mutations in four different loci. Seven distinct mutations affected one locus, coding the MUM2 β-D-galactosidase, and represent a striking example of allelic heterogeneity. The modification of mucilage release has thus evolved a number of times independently in two restricted geographical zones. All the natural mutants identified still accumulated mucilage polysaccharides in seed coat epidermal cells. Using nuclear magnetic resonance (NMR) relaxometry their production and retention was shown to reduce water mobility into internal seed tissues during imbibition, which would help to maintain seed buoyancy. Surprisingly, despite released mucilage being an excellent hydrogel it did not increase the rate of water uptake by internal seed tissues and is more likely to play a role in retaining water around the seed. |
| first_indexed | 2024-04-12T11:06:42Z |
| format | Article |
| id | doaj.art-54f90697de7347a0b45ef635bb56a2d0 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-12T11:06:42Z |
| publishDate | 2014-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-54f90697de7347a0b45ef635bb56a2d02022-12-22T03:35:44ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042014-03-01103e100422110.1371/journal.pgen.1004221Local evolution of seed flotation in Arabidopsis.Susana Saez-AguayoCorinne Rondeau-MouroAudrey MacquetIlkka KronholmMarie-Christine RaletAdeline BergerChristine SalléDamien PoulainFabienne GranierLucy BotranOlivier LoudetJuliette de MeauxAnnie Marion-PollHelen M NorthArabidopsis seeds rapidly release hydrophilic polysaccharides from the seed coat on imbibition. These form a heavy mucilage layer around the seed that makes it sink in water. Fourteen natural Arabidopsis variants from central Asia and Scandinavia were identified with seeds that have modified mucilage release and float. Four of these have a novel mucilage phenotype with almost none of the released mucilage adhering to the seed and the absence of cellulose microfibrils. Mucilage release was modified in the variants by ten independent causal mutations in four different loci. Seven distinct mutations affected one locus, coding the MUM2 β-D-galactosidase, and represent a striking example of allelic heterogeneity. The modification of mucilage release has thus evolved a number of times independently in two restricted geographical zones. All the natural mutants identified still accumulated mucilage polysaccharides in seed coat epidermal cells. Using nuclear magnetic resonance (NMR) relaxometry their production and retention was shown to reduce water mobility into internal seed tissues during imbibition, which would help to maintain seed buoyancy. Surprisingly, despite released mucilage being an excellent hydrogel it did not increase the rate of water uptake by internal seed tissues and is more likely to play a role in retaining water around the seed.http://europepmc.org/articles/PMC3953066?pdf=render |
| spellingShingle | Susana Saez-Aguayo Corinne Rondeau-Mouro Audrey Macquet Ilkka Kronholm Marie-Christine Ralet Adeline Berger Christine Sallé Damien Poulain Fabienne Granier Lucy Botran Olivier Loudet Juliette de Meaux Annie Marion-Poll Helen M North Local evolution of seed flotation in Arabidopsis. PLoS Genetics |
| title | Local evolution of seed flotation in Arabidopsis. |
| title_full | Local evolution of seed flotation in Arabidopsis. |
| title_fullStr | Local evolution of seed flotation in Arabidopsis. |
| title_full_unstemmed | Local evolution of seed flotation in Arabidopsis. |
| title_short | Local evolution of seed flotation in Arabidopsis. |
| title_sort | local evolution of seed flotation in arabidopsis |
| url | http://europepmc.org/articles/PMC3953066?pdf=render |
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