Proteomic profiling identifies biomarkers of COVID-19 severity
SARS-CoV-2 infection remains a major public health concern, particularly for the aged and those individuals with co-morbidities at risk for developing severe COVID-19. Understanding the pathogenesis and biomarkers associated with responses to SARS-CoV-2 infection remain critical components in develo...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-01-01
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| Series: | Heliyon |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023105287 |
| _version_ | 1797337023619530752 |
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| author | Noa C. Harriott Amy L. Ryan |
| author_facet | Noa C. Harriott Amy L. Ryan |
| author_sort | Noa C. Harriott |
| collection | DOAJ |
| description | SARS-CoV-2 infection remains a major public health concern, particularly for the aged and those individuals with co-morbidities at risk for developing severe COVID-19. Understanding the pathogenesis and biomarkers associated with responses to SARS-CoV-2 infection remain critical components in developing effective therapeutic approaches, especially in cases of severe and long-COVID-19. In this study blood plasma protein expression was compared in subjects with mild, moderate, and severe COVID-19 disease. Evaluation of an inflammatory protein panel confirms upregulation of proteins including TNFβ, IL-6, IL-8, IL-12, already associated with severe cytokine storm and progression to severe COVID-19. Importantly, we identify several proteins not yet associated with COVID-19 disease, including mesothelin (MSLN), that are expressed at significantly higher levels in severe COVID-19 subjects. In addition, we find a subset of markers associated with T-cell and dendritic cell responses to viral infection that are significantly higher in mild cases and decrease in expression as severity of COVID-19 increases, suggesting that an immediate and effective activation of T-cells is critical in modulating disease progression. Together, our findings identify new targets for further investigation as therapeutic approaches for the treatment of SARS-CoV-2 infection and prevention of complications of severe COVID-19. |
| first_indexed | 2024-03-08T09:03:23Z |
| format | Article |
| id | doaj.art-54fa297e65f642f0b83a28a6b719f668 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T09:03:23Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-54fa297e65f642f0b83a28a6b719f6682024-02-01T06:31:26ZengElsevierHeliyon2405-84402024-01-01101e23320Proteomic profiling identifies biomarkers of COVID-19 severityNoa C. Harriott0Amy L. Ryan1Hastings Center for Pulmonary Research, Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, University of Southern California, Los Angeles CA 90033, USA; Department of Stem Cell Biology and Regenerative Medicine, University of Southern California, Los Angeles CA 90033, USA; Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City IA 52240, USAHastings Center for Pulmonary Research, Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, University of Southern California, Los Angeles CA 90033, USA; Department of Stem Cell Biology and Regenerative Medicine, University of Southern California, Los Angeles CA 90033, USA; Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City IA 52240, USA; Corresponding author. Anatomy and Cell Biology Associate Director: Center for Gene Therapy BSB, 1-400 Core University of Iowa 51 Newton Road Iowa City, Iowa, 52242, USA.SARS-CoV-2 infection remains a major public health concern, particularly for the aged and those individuals with co-morbidities at risk for developing severe COVID-19. Understanding the pathogenesis and biomarkers associated with responses to SARS-CoV-2 infection remain critical components in developing effective therapeutic approaches, especially in cases of severe and long-COVID-19. In this study blood plasma protein expression was compared in subjects with mild, moderate, and severe COVID-19 disease. Evaluation of an inflammatory protein panel confirms upregulation of proteins including TNFβ, IL-6, IL-8, IL-12, already associated with severe cytokine storm and progression to severe COVID-19. Importantly, we identify several proteins not yet associated with COVID-19 disease, including mesothelin (MSLN), that are expressed at significantly higher levels in severe COVID-19 subjects. In addition, we find a subset of markers associated with T-cell and dendritic cell responses to viral infection that are significantly higher in mild cases and decrease in expression as severity of COVID-19 increases, suggesting that an immediate and effective activation of T-cells is critical in modulating disease progression. Together, our findings identify new targets for further investigation as therapeutic approaches for the treatment of SARS-CoV-2 infection and prevention of complications of severe COVID-19.http://www.sciencedirect.com/science/article/pii/S2405844023105287 |
| spellingShingle | Noa C. Harriott Amy L. Ryan Proteomic profiling identifies biomarkers of COVID-19 severity Heliyon |
| title | Proteomic profiling identifies biomarkers of COVID-19 severity |
| title_full | Proteomic profiling identifies biomarkers of COVID-19 severity |
| title_fullStr | Proteomic profiling identifies biomarkers of COVID-19 severity |
| title_full_unstemmed | Proteomic profiling identifies biomarkers of COVID-19 severity |
| title_short | Proteomic profiling identifies biomarkers of COVID-19 severity |
| title_sort | proteomic profiling identifies biomarkers of covid 19 severity |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023105287 |
| work_keys_str_mv | AT noacharriott proteomicprofilingidentifiesbiomarkersofcovid19severity AT amylryan proteomicprofilingidentifiesbiomarkersofcovid19severity |