Transcriptomic Profile Analysis of <i>Populus talassica</i> × <i>Populus euphratica</i> Response and Tolerance under Salt Stress Conditions
A new <i>Populus</i> variety with a strong salt tolerance was obtained from cross breeding <i>P. talassica</i> as the female parent and <i>P. euphratica</i> as the male parent. In order to elucidate the molecular mechanism and find out the major differentially exp...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/6/1032 |
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| author | Ying Liu Zhan Jiang Han Meng Xu Su Min Zhang |
| author_facet | Ying Liu Zhan Jiang Han Meng Xu Su Min Zhang |
| author_sort | Ying Liu |
| collection | DOAJ |
| description | A new <i>Populus</i> variety with a strong salt tolerance was obtained from cross breeding <i>P. talassica</i> as the female parent and <i>P. euphratica</i> as the male parent. In order to elucidate the molecular mechanism and find out the major differentially expressed genes of salt tolerance of <i>P. talassica × P. euphratica</i>, after being subjected to salt stress, at 0, 200, and 400 mmol/L NaCl, the root, stem, and leaf transcriptomes (denoted as R0, S0, and L0; R200, S200, and L200; and R400, S400, and L400, respectively) of <i>P. talassica</i> × <i>P. euphratica</i> were sequenced. In total, 41,617 differentially expressed genes (DEGs) were identified in all the comparison groups with 21,603 differentially upregulated genes and 20,014 differentially downregulated genes. Gene Ontology analysis showed that DEGs were significantly enriched in biological processes that may be involved in salt stress, such as ‘cell communication’, ‘ion transport’, ‘signaling’, and signal ‘transmission’. Kyoto Encyclopedia of Genes and Genomes analysis showed that DEGs were mainly enriched in pathways of ‘plant–pathogen interaction’, ‘carbon metabolism’, and ‘plant hormone signal transmission’. The pathways and related gene information formed a basis for future research on the mechanisms of salt stress, the development of molecular markers, and the cloning of key genes in <i>P. talassica</i> × <i>P. euphratica</i>. |
| first_indexed | 2024-03-09T23:42:48Z |
| format | Article |
| id | doaj.art-54fc798501b342c394b45b5411f081d8 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T23:42:48Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-54fc798501b342c394b45b5411f081d82023-11-23T16:48:10ZengMDPI AGGenes2073-44252022-06-01136103210.3390/genes13061032Transcriptomic Profile Analysis of <i>Populus talassica</i> × <i>Populus euphratica</i> Response and Tolerance under Salt Stress ConditionsYing Liu0Zhan Jiang Han1Meng Xu Su2Min Zhang3College of Life Science and Technology, Tarim University, Alar 843300, ChinaCollege of Life Science and Technology, Tarim University, Alar 843300, ChinaCollege of Life Science and Technology, Tarim University, Alar 843300, ChinaCollege of Life Science and Technology, Tarim University, Alar 843300, ChinaA new <i>Populus</i> variety with a strong salt tolerance was obtained from cross breeding <i>P. talassica</i> as the female parent and <i>P. euphratica</i> as the male parent. In order to elucidate the molecular mechanism and find out the major differentially expressed genes of salt tolerance of <i>P. talassica × P. euphratica</i>, after being subjected to salt stress, at 0, 200, and 400 mmol/L NaCl, the root, stem, and leaf transcriptomes (denoted as R0, S0, and L0; R200, S200, and L200; and R400, S400, and L400, respectively) of <i>P. talassica</i> × <i>P. euphratica</i> were sequenced. In total, 41,617 differentially expressed genes (DEGs) were identified in all the comparison groups with 21,603 differentially upregulated genes and 20,014 differentially downregulated genes. Gene Ontology analysis showed that DEGs were significantly enriched in biological processes that may be involved in salt stress, such as ‘cell communication’, ‘ion transport’, ‘signaling’, and signal ‘transmission’. Kyoto Encyclopedia of Genes and Genomes analysis showed that DEGs were mainly enriched in pathways of ‘plant–pathogen interaction’, ‘carbon metabolism’, and ‘plant hormone signal transmission’. The pathways and related gene information formed a basis for future research on the mechanisms of salt stress, the development of molecular markers, and the cloning of key genes in <i>P. talassica</i> × <i>P. euphratica</i>.https://www.mdpi.com/2073-4425/13/6/1032<i>Populus talassica</i> <i>× Populus euphratica</i>salt stresstranscriptome sequencingdifferentially expressed genes |
| spellingShingle | Ying Liu Zhan Jiang Han Meng Xu Su Min Zhang Transcriptomic Profile Analysis of <i>Populus talassica</i> × <i>Populus euphratica</i> Response and Tolerance under Salt Stress Conditions Genes <i>Populus talassica</i> <i>× Populus euphratica</i> salt stress transcriptome sequencing differentially expressed genes |
| title | Transcriptomic Profile Analysis of <i>Populus talassica</i> × <i>Populus euphratica</i> Response and Tolerance under Salt Stress Conditions |
| title_full | Transcriptomic Profile Analysis of <i>Populus talassica</i> × <i>Populus euphratica</i> Response and Tolerance under Salt Stress Conditions |
| title_fullStr | Transcriptomic Profile Analysis of <i>Populus talassica</i> × <i>Populus euphratica</i> Response and Tolerance under Salt Stress Conditions |
| title_full_unstemmed | Transcriptomic Profile Analysis of <i>Populus talassica</i> × <i>Populus euphratica</i> Response and Tolerance under Salt Stress Conditions |
| title_short | Transcriptomic Profile Analysis of <i>Populus talassica</i> × <i>Populus euphratica</i> Response and Tolerance under Salt Stress Conditions |
| title_sort | transcriptomic profile analysis of i populus talassica i i populus euphratica i response and tolerance under salt stress conditions |
| topic | <i>Populus talassica</i> <i>× Populus euphratica</i> salt stress transcriptome sequencing differentially expressed genes |
| url | https://www.mdpi.com/2073-4425/13/6/1032 |
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