Characterization of leucocin B-KM432Bz from Leuconostoc pseudomesenteroides isolated from boza, and comparison of its efficiency to pediocin PA-1.
A bacteriocin-producing bacterium was isolated from boza and identified as Leuconostoc pseudomesenteroides KM432Bz. The antimicrobial peptide was purified and shown to be identical to other class IIa bacteriocins: leucocin A from Leuconostoc gelidum UAL-187 and Leuconostoc pseudomesenteroides QU15 a...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3731274?pdf=render |
| _version_ | 1818296549719932928 |
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| author | Kahina Maya Makhloufi Alyssa Carré-Mlouka Jean Peduzzi Carine Lombard Carol Ann van Reenen Leon Milner Theodore Dicks Sylvie Rebuffat |
| author_facet | Kahina Maya Makhloufi Alyssa Carré-Mlouka Jean Peduzzi Carine Lombard Carol Ann van Reenen Leon Milner Theodore Dicks Sylvie Rebuffat |
| author_sort | Kahina Maya Makhloufi |
| collection | DOAJ |
| description | A bacteriocin-producing bacterium was isolated from boza and identified as Leuconostoc pseudomesenteroides KM432Bz. The antimicrobial peptide was purified and shown to be identical to other class IIa bacteriocins: leucocin A from Leuconostoc gelidum UAL-187 and Leuconostoc pseudomesenteroides QU15 and leucocin B from Leuconostoc carnosum Ta11a. The bacteriocin was named leucocin B-KM432Bz. Leucocin B-KM432Bz gene cluster encodes the bacteriocin precursor (lcnB), the immunity protein (lcnI) and the dedicated export machinery (lcnD and lcnE). A gene of unknown and non-essential function (lcnC), which is interrupted by an insertion sequence of the IS30 family, is localized between lcnB and lcnD. The activity of leucocin B-KM432Bz requires subunit C of the EII(t) Man mannose permease, which is the receptor for entry into target cells. The determination of the minimum inhibitory concentrations revealed the lowest values for leucocin B-KM432Bz over Listeria strains, with 4 to 32 fold better efficiency than pediocin PA-1. |
| first_indexed | 2024-12-13T04:05:19Z |
| format | Article |
| id | doaj.art-54fce70a6e0e4d0e979eee4716c59818 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T04:05:19Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-54fce70a6e0e4d0e979eee4716c598182022-12-22T00:00:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7048410.1371/journal.pone.0070484Characterization of leucocin B-KM432Bz from Leuconostoc pseudomesenteroides isolated from boza, and comparison of its efficiency to pediocin PA-1.Kahina Maya MakhloufiAlyssa Carré-MloukaJean PeduzziCarine LombardCarol Ann van ReenenLeon Milner Theodore DicksSylvie RebuffatA bacteriocin-producing bacterium was isolated from boza and identified as Leuconostoc pseudomesenteroides KM432Bz. The antimicrobial peptide was purified and shown to be identical to other class IIa bacteriocins: leucocin A from Leuconostoc gelidum UAL-187 and Leuconostoc pseudomesenteroides QU15 and leucocin B from Leuconostoc carnosum Ta11a. The bacteriocin was named leucocin B-KM432Bz. Leucocin B-KM432Bz gene cluster encodes the bacteriocin precursor (lcnB), the immunity protein (lcnI) and the dedicated export machinery (lcnD and lcnE). A gene of unknown and non-essential function (lcnC), which is interrupted by an insertion sequence of the IS30 family, is localized between lcnB and lcnD. The activity of leucocin B-KM432Bz requires subunit C of the EII(t) Man mannose permease, which is the receptor for entry into target cells. The determination of the minimum inhibitory concentrations revealed the lowest values for leucocin B-KM432Bz over Listeria strains, with 4 to 32 fold better efficiency than pediocin PA-1.http://europepmc.org/articles/PMC3731274?pdf=render |
| spellingShingle | Kahina Maya Makhloufi Alyssa Carré-Mlouka Jean Peduzzi Carine Lombard Carol Ann van Reenen Leon Milner Theodore Dicks Sylvie Rebuffat Characterization of leucocin B-KM432Bz from Leuconostoc pseudomesenteroides isolated from boza, and comparison of its efficiency to pediocin PA-1. PLoS ONE |
| title | Characterization of leucocin B-KM432Bz from Leuconostoc pseudomesenteroides isolated from boza, and comparison of its efficiency to pediocin PA-1. |
| title_full | Characterization of leucocin B-KM432Bz from Leuconostoc pseudomesenteroides isolated from boza, and comparison of its efficiency to pediocin PA-1. |
| title_fullStr | Characterization of leucocin B-KM432Bz from Leuconostoc pseudomesenteroides isolated from boza, and comparison of its efficiency to pediocin PA-1. |
| title_full_unstemmed | Characterization of leucocin B-KM432Bz from Leuconostoc pseudomesenteroides isolated from boza, and comparison of its efficiency to pediocin PA-1. |
| title_short | Characterization of leucocin B-KM432Bz from Leuconostoc pseudomesenteroides isolated from boza, and comparison of its efficiency to pediocin PA-1. |
| title_sort | characterization of leucocin b km432bz from leuconostoc pseudomesenteroides isolated from boza and comparison of its efficiency to pediocin pa 1 |
| url | http://europepmc.org/articles/PMC3731274?pdf=render |
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