Evaluation of α-Glucosidase Inhibition and Antihyperglycemic Activity of Extracts Obtained from Leaves and Flowers of <i>Rumex crispus</i> L.
Among antihyperglycemic drugs used for treating diabetes, α-glucosidase inhibitors generate the least adverse effects. This contribution aimed to evaluate the potential antidiabetic activity of <i>Rumex crispus</i> L. by testing its in vitro α-glucosidase inhibition and in vivo antihyper...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/15/5760 |
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| author | Dolores G. Aguila-Muñoz Fabiola E. Jiménez-Montejo Víctor E. López-López Aarón Mendieta-Moctezuma Jorge Rodríguez-Antolín Jorge Cornejo-Garrido María C. Cruz-López |
| author_facet | Dolores G. Aguila-Muñoz Fabiola E. Jiménez-Montejo Víctor E. López-López Aarón Mendieta-Moctezuma Jorge Rodríguez-Antolín Jorge Cornejo-Garrido María C. Cruz-López |
| author_sort | Dolores G. Aguila-Muñoz |
| collection | DOAJ |
| description | Among antihyperglycemic drugs used for treating diabetes, α-glucosidase inhibitors generate the least adverse effects. This contribution aimed to evaluate the potential antidiabetic activity of <i>Rumex crispus</i> L. by testing its in vitro α-glucosidase inhibition and in vivo antihyperglycemic effects on rats with streptozotocin (STZ)-induced diabetes. Better inhibition of α-glucosidase was found with the methanol extract versus the n-hexane and dichloromethane extracts. The methanol extract of the flowers (RCFM) was more effective than that of the leaves (RCHM), with an IC<sub>50</sub> of 7.3 ± 0.17 μg/mL for RCFM and 112.0 ± 1.23 μg/mL for RCHM. A bioactive fraction (F89s) also showed good α-glucosidase inhibition (IC<sub>50</sub> = 3.8 ± 0.11 μg/mL). In a preliminary study, RCHM and RCFM at 150 mg/kg and F89s at 75 mg/kg after 30 days showed a significant effect on hyperglycemia, reducing glucose levels (82.2, 80.1, and 84.1%, respectively), and improved the lipid, renal, and hepatic profiles of the rats, comparable with the effects of metformin and acarbose. According to the results, the activity of <i>R. crispus</i> L. may be mediated by a diminished rate of disaccharide hydrolysis, associated with the inhibition of α-glucosidase. Thus, <i>R. crispus</i> L. holds promise for the development of auxiliary drugs to treat diabetes mellitus. |
| first_indexed | 2024-03-11T00:20:26Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T00:20:26Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-55005807beab42e8b57da9390586d9332023-11-18T23:18:31ZengMDPI AGMolecules1420-30492023-07-012815576010.3390/molecules28155760Evaluation of α-Glucosidase Inhibition and Antihyperglycemic Activity of Extracts Obtained from Leaves and Flowers of <i>Rumex crispus</i> L.Dolores G. Aguila-Muñoz0Fabiola E. Jiménez-Montejo1Víctor E. López-López2Aarón Mendieta-Moctezuma3Jorge Rodríguez-Antolín4Jorge Cornejo-Garrido5María C. Cruz-López6Centro de Investigación en Biotecnología Aplicada, Instituto Politécnico Nacional, Tlaxcala 90700, MexicoCentro de Investigación en Biotecnología Aplicada, Instituto Politécnico Nacional, Tlaxcala 90700, MexicoCentro de Investigación en Biotecnología Aplicada, Instituto Politécnico Nacional, Tlaxcala 90700, MexicoCentro de Investigación en Biotecnología Aplicada, Instituto Politécnico Nacional, Tlaxcala 90700, MexicoCentro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala 90070, MexicoLaboratorio de Biología Celular y Productos Naturales, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Ciudad de México 07320, MexicoCentro de Investigación en Biotecnología Aplicada, Instituto Politécnico Nacional, Tlaxcala 90700, MexicoAmong antihyperglycemic drugs used for treating diabetes, α-glucosidase inhibitors generate the least adverse effects. This contribution aimed to evaluate the potential antidiabetic activity of <i>Rumex crispus</i> L. by testing its in vitro α-glucosidase inhibition and in vivo antihyperglycemic effects on rats with streptozotocin (STZ)-induced diabetes. Better inhibition of α-glucosidase was found with the methanol extract versus the n-hexane and dichloromethane extracts. The methanol extract of the flowers (RCFM) was more effective than that of the leaves (RCHM), with an IC<sub>50</sub> of 7.3 ± 0.17 μg/mL for RCFM and 112.0 ± 1.23 μg/mL for RCHM. A bioactive fraction (F89s) also showed good α-glucosidase inhibition (IC<sub>50</sub> = 3.8 ± 0.11 μg/mL). In a preliminary study, RCHM and RCFM at 150 mg/kg and F89s at 75 mg/kg after 30 days showed a significant effect on hyperglycemia, reducing glucose levels (82.2, 80.1, and 84.1%, respectively), and improved the lipid, renal, and hepatic profiles of the rats, comparable with the effects of metformin and acarbose. According to the results, the activity of <i>R. crispus</i> L. may be mediated by a diminished rate of disaccharide hydrolysis, associated with the inhibition of α-glucosidase. Thus, <i>R. crispus</i> L. holds promise for the development of auxiliary drugs to treat diabetes mellitus.https://www.mdpi.com/1420-3049/28/15/5760<i>Rumex crispus</i> L.<i>α</i>-glucosidase inhibitorsantihyperglycemic activitydiabetesbioassay-guided study |
| spellingShingle | Dolores G. Aguila-Muñoz Fabiola E. Jiménez-Montejo Víctor E. López-López Aarón Mendieta-Moctezuma Jorge Rodríguez-Antolín Jorge Cornejo-Garrido María C. Cruz-López Evaluation of α-Glucosidase Inhibition and Antihyperglycemic Activity of Extracts Obtained from Leaves and Flowers of <i>Rumex crispus</i> L. Molecules <i>Rumex crispus</i> L. <i>α</i>-glucosidase inhibitors antihyperglycemic activity diabetes bioassay-guided study |
| title | Evaluation of α-Glucosidase Inhibition and Antihyperglycemic Activity of Extracts Obtained from Leaves and Flowers of <i>Rumex crispus</i> L. |
| title_full | Evaluation of α-Glucosidase Inhibition and Antihyperglycemic Activity of Extracts Obtained from Leaves and Flowers of <i>Rumex crispus</i> L. |
| title_fullStr | Evaluation of α-Glucosidase Inhibition and Antihyperglycemic Activity of Extracts Obtained from Leaves and Flowers of <i>Rumex crispus</i> L. |
| title_full_unstemmed | Evaluation of α-Glucosidase Inhibition and Antihyperglycemic Activity of Extracts Obtained from Leaves and Flowers of <i>Rumex crispus</i> L. |
| title_short | Evaluation of α-Glucosidase Inhibition and Antihyperglycemic Activity of Extracts Obtained from Leaves and Flowers of <i>Rumex crispus</i> L. |
| title_sort | evaluation of α glucosidase inhibition and antihyperglycemic activity of extracts obtained from leaves and flowers of i rumex crispus i l |
| topic | <i>Rumex crispus</i> L. <i>α</i>-glucosidase inhibitors antihyperglycemic activity diabetes bioassay-guided study |
| url | https://www.mdpi.com/1420-3049/28/15/5760 |
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