Reference gene identification for reliable normalisation of quantitative RT-PCR data in Setaria viridis

Reference gene identification for reliable normalisation of quantitative RT-PCR data in Setaria viridis

Abstract Background Quantitative real-time polymerase chain reaction (RT-qPCR) is the key platform for the quantitative analysis of gene expression in a wide range of experimental systems and conditions. However, the accuracy and reproducibility of gene expression quantification via RT-qPCR is entir...

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Bibliographic Details
Main Authors: Duc Quan Nguyen, Andrew L. Eamens, Christopher P. L. Grof
Format: Article
Language:English
Published: BMC 2018-03-01
Series:Plant Methods
Subjects:
Setaria viridis
C4 crop
Quantitative RT-PCR normalisation
Reference genes
Gene expression
Online Access:http://link.springer.com/article/10.1186/s13007-018-0293-8

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