QUANTITATIF ANALYSIS OF TOTAL FLAVONOID LEVEL AND ANTIOXIDANT ACTIVITY TEST PURPLE CABBAGE (Brassica oleracea L. var. capitata f. rubra) AND WHITE CABBAGE (Brassica oleracea L. var. capitata f. alba) ETHANOL EXTRACT USING DPPH METHOD (1,1-difenil-2-pikril
Antioxidants are compounds that are needed by the body to protect the body from free radical attacks that can trigger the emergence of degenerative diseases. One of the compounds that has free radical scavenger activity by inhibiting Reactive Oxygen Species (ROS) is flavonoids found in purple cabbag...
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| Format: | Article |
| Language: | Indonesian |
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Universitas Muhammadiyah Magelang
2021-11-01
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| Series: | Jurnal Farmasi Sains dan Praktis |
| Subjects: | |
| Online Access: | https://journal.unimma.ac.id/index.php/pharmacy/article/view/4369 |
| _version_ | 1811191344827203584 |
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| author | Any Guntarti Ratna Yuningtyas Hari Susanti Zainab Zainab |
| author_facet | Any Guntarti Ratna Yuningtyas Hari Susanti Zainab Zainab |
| author_sort | Any Guntarti |
| collection | DOAJ |
| description | Antioxidants are compounds that are needed by the body to protect the body from free radical attacks that can trigger the emergence of degenerative diseases. One of the compounds that has free radical scavenger activity by inhibiting Reactive Oxygen Species (ROS) is flavonoids found in purple cabbage and white cabbage. This study aims to determine the total levels of flavonoids expressed as quercetin equivalent (EK) and antioxidant activity using the DPPH method (1,1-diphenyl-2- picrylhydrazyl). Identification of flavonoid compounds by Willstater test and determination of total flavonoid levels using spectrophotometry, AlCl3 reagent. Flavonoid levels were calculated using linear regression equations. Qualitative test for the presence of antioxidant activity using TLC and antioxidant activity test using the DPPH method with the parameter value of ES50. The qualitative test results showed that the ethanol extract of purple cabbage and white cabbage contained flavonoids. The total flavonoid content of ethanol extract of purple cabbage was 5.17% w/w (EK) and ethanol extract of white cabbage was 3.84% w/w (EK). Qualitative test with TLC showed antioxidant activity. The standard antioxidant activity values of quercetin, ethanol extract of purple cabbage and white cabbage were 2.138±0.064 µg/mL; 154.445±0,999 µg/mL and 373.546±1.336 µg/mL. The antioxidant activity of purple cabbage is weak and white cabbage is very weak. |
| first_indexed | 2024-04-11T15:03:50Z |
| format | Article |
| id | doaj.art-5504dd80717a454b941e0afa213e09a7 |
| institution | Directory Open Access Journal |
| issn | 2549-9068 2579-4558 |
| language | Indonesian |
| last_indexed | 2024-04-11T15:03:50Z |
| publishDate | 2021-11-01 |
| publisher | Universitas Muhammadiyah Magelang |
| record_format | Article |
| series | Jurnal Farmasi Sains dan Praktis |
| spelling | doaj.art-5504dd80717a454b941e0afa213e09a72022-12-22T04:16:51ZindUniversitas Muhammadiyah MagelangJurnal Farmasi Sains dan Praktis2549-90682579-45582021-11-017210.31603/pharmacy.v7i2.4369QUANTITATIF ANALYSIS OF TOTAL FLAVONOID LEVEL AND ANTIOXIDANT ACTIVITY TEST PURPLE CABBAGE (Brassica oleracea L. var. capitata f. rubra) AND WHITE CABBAGE (Brassica oleracea L. var. capitata f. alba) ETHANOL EXTRACT USING DPPH METHOD (1,1-difenil-2-pikrilAny Guntarti0Ratna Yuningtyas1Hari Susanti2Zainab Zainab3Universitas Ahmad DahlanUniversitas Ahmad DahlanUniversitas Ahmad DahlanUniversitas Ahmad DahlanAntioxidants are compounds that are needed by the body to protect the body from free radical attacks that can trigger the emergence of degenerative diseases. One of the compounds that has free radical scavenger activity by inhibiting Reactive Oxygen Species (ROS) is flavonoids found in purple cabbage and white cabbage. This study aims to determine the total levels of flavonoids expressed as quercetin equivalent (EK) and antioxidant activity using the DPPH method (1,1-diphenyl-2- picrylhydrazyl). Identification of flavonoid compounds by Willstater test and determination of total flavonoid levels using spectrophotometry, AlCl3 reagent. Flavonoid levels were calculated using linear regression equations. Qualitative test for the presence of antioxidant activity using TLC and antioxidant activity test using the DPPH method with the parameter value of ES50. The qualitative test results showed that the ethanol extract of purple cabbage and white cabbage contained flavonoids. The total flavonoid content of ethanol extract of purple cabbage was 5.17% w/w (EK) and ethanol extract of white cabbage was 3.84% w/w (EK). Qualitative test with TLC showed antioxidant activity. The standard antioxidant activity values of quercetin, ethanol extract of purple cabbage and white cabbage were 2.138±0.064 µg/mL; 154.445±0,999 µg/mL and 373.546±1.336 µg/mL. The antioxidant activity of purple cabbage is weak and white cabbage is very weak.https://journal.unimma.ac.id/index.php/pharmacy/article/view/4369AntioxidantPurple cabbageWhite cabbageTotal flavonoidsDPPH |
| spellingShingle | Any Guntarti Ratna Yuningtyas Hari Susanti Zainab Zainab QUANTITATIF ANALYSIS OF TOTAL FLAVONOID LEVEL AND ANTIOXIDANT ACTIVITY TEST PURPLE CABBAGE (Brassica oleracea L. var. capitata f. rubra) AND WHITE CABBAGE (Brassica oleracea L. var. capitata f. alba) ETHANOL EXTRACT USING DPPH METHOD (1,1-difenil-2-pikril Jurnal Farmasi Sains dan Praktis Antioxidant Purple cabbage White cabbage Total flavonoids DPPH |
| title | QUANTITATIF ANALYSIS OF TOTAL FLAVONOID LEVEL AND ANTIOXIDANT ACTIVITY TEST PURPLE CABBAGE (Brassica oleracea L. var. capitata f. rubra) AND WHITE CABBAGE (Brassica oleracea L. var. capitata f. alba) ETHANOL EXTRACT USING DPPH METHOD (1,1-difenil-2-pikril |
| title_full | QUANTITATIF ANALYSIS OF TOTAL FLAVONOID LEVEL AND ANTIOXIDANT ACTIVITY TEST PURPLE CABBAGE (Brassica oleracea L. var. capitata f. rubra) AND WHITE CABBAGE (Brassica oleracea L. var. capitata f. alba) ETHANOL EXTRACT USING DPPH METHOD (1,1-difenil-2-pikril |
| title_fullStr | QUANTITATIF ANALYSIS OF TOTAL FLAVONOID LEVEL AND ANTIOXIDANT ACTIVITY TEST PURPLE CABBAGE (Brassica oleracea L. var. capitata f. rubra) AND WHITE CABBAGE (Brassica oleracea L. var. capitata f. alba) ETHANOL EXTRACT USING DPPH METHOD (1,1-difenil-2-pikril |
| title_full_unstemmed | QUANTITATIF ANALYSIS OF TOTAL FLAVONOID LEVEL AND ANTIOXIDANT ACTIVITY TEST PURPLE CABBAGE (Brassica oleracea L. var. capitata f. rubra) AND WHITE CABBAGE (Brassica oleracea L. var. capitata f. alba) ETHANOL EXTRACT USING DPPH METHOD (1,1-difenil-2-pikril |
| title_short | QUANTITATIF ANALYSIS OF TOTAL FLAVONOID LEVEL AND ANTIOXIDANT ACTIVITY TEST PURPLE CABBAGE (Brassica oleracea L. var. capitata f. rubra) AND WHITE CABBAGE (Brassica oleracea L. var. capitata f. alba) ETHANOL EXTRACT USING DPPH METHOD (1,1-difenil-2-pikril |
| title_sort | quantitatif analysis of total flavonoid level and antioxidant activity test purple cabbage brassica oleracea l var capitata f rubra and white cabbage brassica oleracea l var capitata f alba ethanol extract using dpph method 1 1 difenil 2 pikril |
| topic | Antioxidant Purple cabbage White cabbage Total flavonoids DPPH |
| url | https://journal.unimma.ac.id/index.php/pharmacy/article/view/4369 |
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