Transcriptomics Reveals Fast Changes in Salicylate and Jasmonate Signaling Pathways in Shoots of Carbonate-Tolerant <i>Arabidopsis thaliana</i> under Bicarbonate Exposure
High bicarbonate concentrations of calcareous soils with high pH can affect crop performance due to different constraints. Among these, Fe deficiency has mostly been studied. The ability to mobilize sparingly soluble Fe is a key factor for tolerance. Here, a comparative transcriptomic analysis was p...
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/3/1226 |
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| author | Laura Pérez-Martín Silvia Busoms Roser Tolrà Charlotte Poschenrieder |
| author_facet | Laura Pérez-Martín Silvia Busoms Roser Tolrà Charlotte Poschenrieder |
| author_sort | Laura Pérez-Martín |
| collection | DOAJ |
| description | High bicarbonate concentrations of calcareous soils with high pH can affect crop performance due to different constraints. Among these, Fe deficiency has mostly been studied. The ability to mobilize sparingly soluble Fe is a key factor for tolerance. Here, a comparative transcriptomic analysis was performed with two naturally selected <i>Arabidopsis thaliana</i> demes, the carbonate-tolerant A1<sub>(c+)</sub> and the sensitive T6<sub>(c−)</sub>. Analyses of plants exposed to either pH stress alone (pH 5.9 vs. pH 8.3) or to alkalinity caused by 10 mM NaHCO<sub>3</sub> (pH 8.3) confirmed better growth and nutrient homeostasis of A1<sub>(c+)</sub> under alkaline conditions. RNA-sequencing (RNA-seq) revealed that bicarbonate quickly (3 h) induced Fe deficiency-related genes in T6<sub>(c−)</sub> leaves. Contrastingly, in A1<sub>(c+)</sub>, initial changes concerned receptor-like proteins (RLP), jasmonate (JA) and salicylate (SA) pathways, methionine-derived glucosinolates (GS), sulfur starvation, starch degradation, and cell cycle. Our results suggest that leaves of carbonate-tolerant plants do not sense iron deficiency as fast as sensitive ones. This is in line with a more efficient Fe translocation to aerial parts. In A1<sub>(c+)</sub> leaves, the activation of other genes related to stress perception, signal transduction, GS, sulfur acquisition, and cell cycle precedes the induction of iron homeostasis mechanisms yielding an efficient response to bicarbonate stress. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T03:33:24Z |
| publishDate | 2021-01-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-55053ffab30d42f282dd4124f7512ffa2023-12-03T14:51:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-01223122610.3390/ijms22031226Transcriptomics Reveals Fast Changes in Salicylate and Jasmonate Signaling Pathways in Shoots of Carbonate-Tolerant <i>Arabidopsis thaliana</i> under Bicarbonate ExposureLaura Pérez-Martín0Silvia Busoms1Roser Tolrà2Charlotte Poschenrieder3Plant Physiology Laboratory, Bioscience Faculty, Universitat Autònoma de Barcelona, C/de la Vall Moronta s/n, E-08193 Bellaterra, SpainPlant Physiology Laboratory, Bioscience Faculty, Universitat Autònoma de Barcelona, C/de la Vall Moronta s/n, E-08193 Bellaterra, SpainPlant Physiology Laboratory, Bioscience Faculty, Universitat Autònoma de Barcelona, C/de la Vall Moronta s/n, E-08193 Bellaterra, SpainPlant Physiology Laboratory, Bioscience Faculty, Universitat Autònoma de Barcelona, C/de la Vall Moronta s/n, E-08193 Bellaterra, SpainHigh bicarbonate concentrations of calcareous soils with high pH can affect crop performance due to different constraints. Among these, Fe deficiency has mostly been studied. The ability to mobilize sparingly soluble Fe is a key factor for tolerance. Here, a comparative transcriptomic analysis was performed with two naturally selected <i>Arabidopsis thaliana</i> demes, the carbonate-tolerant A1<sub>(c+)</sub> and the sensitive T6<sub>(c−)</sub>. Analyses of plants exposed to either pH stress alone (pH 5.9 vs. pH 8.3) or to alkalinity caused by 10 mM NaHCO<sub>3</sub> (pH 8.3) confirmed better growth and nutrient homeostasis of A1<sub>(c+)</sub> under alkaline conditions. RNA-sequencing (RNA-seq) revealed that bicarbonate quickly (3 h) induced Fe deficiency-related genes in T6<sub>(c−)</sub> leaves. Contrastingly, in A1<sub>(c+)</sub>, initial changes concerned receptor-like proteins (RLP), jasmonate (JA) and salicylate (SA) pathways, methionine-derived glucosinolates (GS), sulfur starvation, starch degradation, and cell cycle. Our results suggest that leaves of carbonate-tolerant plants do not sense iron deficiency as fast as sensitive ones. This is in line with a more efficient Fe translocation to aerial parts. In A1<sub>(c+)</sub> leaves, the activation of other genes related to stress perception, signal transduction, GS, sulfur acquisition, and cell cycle precedes the induction of iron homeostasis mechanisms yielding an efficient response to bicarbonate stress.https://www.mdpi.com/1422-0067/22/3/1226transcriptomic<i>Arabidopsis thaliana</i>alkaline stressglucosinolatesalicylate |
| spellingShingle | Laura Pérez-Martín Silvia Busoms Roser Tolrà Charlotte Poschenrieder Transcriptomics Reveals Fast Changes in Salicylate and Jasmonate Signaling Pathways in Shoots of Carbonate-Tolerant <i>Arabidopsis thaliana</i> under Bicarbonate Exposure International Journal of Molecular Sciences transcriptomic <i>Arabidopsis thaliana</i> alkaline stress glucosinolate salicylate |
| title | Transcriptomics Reveals Fast Changes in Salicylate and Jasmonate Signaling Pathways in Shoots of Carbonate-Tolerant <i>Arabidopsis thaliana</i> under Bicarbonate Exposure |
| title_full | Transcriptomics Reveals Fast Changes in Salicylate and Jasmonate Signaling Pathways in Shoots of Carbonate-Tolerant <i>Arabidopsis thaliana</i> under Bicarbonate Exposure |
| title_fullStr | Transcriptomics Reveals Fast Changes in Salicylate and Jasmonate Signaling Pathways in Shoots of Carbonate-Tolerant <i>Arabidopsis thaliana</i> under Bicarbonate Exposure |
| title_full_unstemmed | Transcriptomics Reveals Fast Changes in Salicylate and Jasmonate Signaling Pathways in Shoots of Carbonate-Tolerant <i>Arabidopsis thaliana</i> under Bicarbonate Exposure |
| title_short | Transcriptomics Reveals Fast Changes in Salicylate and Jasmonate Signaling Pathways in Shoots of Carbonate-Tolerant <i>Arabidopsis thaliana</i> under Bicarbonate Exposure |
| title_sort | transcriptomics reveals fast changes in salicylate and jasmonate signaling pathways in shoots of carbonate tolerant i arabidopsis thaliana i under bicarbonate exposure |
| topic | transcriptomic <i>Arabidopsis thaliana</i> alkaline stress glucosinolate salicylate |
| url | https://www.mdpi.com/1422-0067/22/3/1226 |
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