Systematic Review of PCR Proof of Parvovirus B19 Genomes in Endomyocardial Biopsies of Patients Presenting with Myocarditis or Dilated Cardiomyopathy
Background: Diverse viral infections have been associated with myocarditis (MC) and dilated cardiomyopathy (DCM). In this meta-analysis, we summarize the published results on the association of parvovirus B19 (B19V) genomes with human MC/DCM versus controls. Methods: <i>n</i> = 197 publi...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-06-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/11/6/566 |
| _version_ | 1811226198960766976 |
|---|---|
| author | Angelos G. Rigopoulos Bianca Klutt Marios Matiakis Athanasios Apostolou Sophie Mavrogeni Michel Noutsias |
| author_facet | Angelos G. Rigopoulos Bianca Klutt Marios Matiakis Athanasios Apostolou Sophie Mavrogeni Michel Noutsias |
| author_sort | Angelos G. Rigopoulos |
| collection | DOAJ |
| description | Background: Diverse viral infections have been associated with myocarditis (MC) and dilated cardiomyopathy (DCM). In this meta-analysis, we summarize the published results on the association of parvovirus B19 (B19V) genomes with human MC/DCM versus controls. Methods: <i>n</i> = 197 publications referring to B19V and MC or DCM were retrieved using multiple PubMed search modes. Out of these, <i>n</i> = 29 publications met the inclusion criteria with data from prospective analyses on >10 unselected patients presenting with MC or DCM (dataset: MA01). Data retrieved simultaneously from both controls and MC/DCM patients were available from <i>n</i> = 8 from these publications (dataset: MA02). Results: In the dataset MA01 B19V genomes were detected in 42.6% of the endomyocardial biopsies (EMB) in this cohort by PCR. In the dataset MA02 comprising <i>n</i> = 638 subjects, there was no statistically significant different rate of B19V positivity in myocardial tissues comparing controls (mean: 38.8 + 24.1%) versus the MC/DCM-patients (45.5 + 24.3%; <i>p</i> = 0.58). There was also no statistical difference between the positivity rate of B19V genomes in myocardial tissues of MA01 (46.0 + 19.5%) and the two patient groups of MA02 (<i>p</i> > 0.05). Conclusions: This systematic review reveals that the mean rate of PCR detected B19V genomes in patients presenting with MC/DCM does not differ significantly from the findings in control myocardial tissues. These data imply pathogenetically insignificant latency of B19V genomes in a proportion of myocardial tissues, both in MC-/DCM-patients and in controls. More information (i.e., replicative status, viral protein expression) is pertinent to achieve a comprehensive workup of myocardial B19V infection. |
| first_indexed | 2024-04-12T09:20:20Z |
| format | Article |
| id | doaj.art-5505c1729830407bbfbe3f81d08317ae |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-04-12T09:20:20Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-5505c1729830407bbfbe3f81d08317ae2022-12-22T03:38:39ZengMDPI AGViruses1999-49152019-06-0111656610.3390/v11060566v11060566Systematic Review of PCR Proof of Parvovirus B19 Genomes in Endomyocardial Biopsies of Patients Presenting with Myocarditis or Dilated CardiomyopathyAngelos G. Rigopoulos0Bianca Klutt1Marios Matiakis2Athanasios Apostolou3Sophie Mavrogeni4Michel Noutsias5Mid-German Heart Center, Department of Internal Medicine III (KIM-III), Division of Cardiology, Angiology and Intensive Medical Care, University Hospital Halle-Wittenberg, Mid-German Heart Center, Martin-Luther-University Halle-Wittenberg, Ernst-Grube-Straße 40, D-06120 Halle (Saale), GermanyDepartment of Anesthesiology, Helios Hospital Herzberg/Osterode, Dr.-Frössel-Allee, D-37412 Herzberg am Harz, GermanyMid-German Heart Center, Department of Internal Medicine III (KIM-III), Division of Cardiology, Angiology and Intensive Medical Care, University Hospital Halle-Wittenberg, Mid-German Heart Center, Martin-Luther-University Halle-Wittenberg, Ernst-Grube-Straße 40, D-06120 Halle (Saale), GermanySchool of Health Sciences, Faculty of Medicine, Department of Immunology & Histocompatibility, University of Thessaly, 41500 Larissa, GreeceOnassis Cardiac Surgery Center, Department of Cardiology, and National Kapodistrian University of Athens, Leoforou Andrea Siggrou 356, Kallithea, 17674 Athens, GreeceMid-German Heart Center, Department of Internal Medicine III (KIM-III), Division of Cardiology, Angiology and Intensive Medical Care, University Hospital Halle-Wittenberg, Mid-German Heart Center, Martin-Luther-University Halle-Wittenberg, Ernst-Grube-Straße 40, D-06120 Halle (Saale), GermanyBackground: Diverse viral infections have been associated with myocarditis (MC) and dilated cardiomyopathy (DCM). In this meta-analysis, we summarize the published results on the association of parvovirus B19 (B19V) genomes with human MC/DCM versus controls. Methods: <i>n</i> = 197 publications referring to B19V and MC or DCM were retrieved using multiple PubMed search modes. Out of these, <i>n</i> = 29 publications met the inclusion criteria with data from prospective analyses on >10 unselected patients presenting with MC or DCM (dataset: MA01). Data retrieved simultaneously from both controls and MC/DCM patients were available from <i>n</i> = 8 from these publications (dataset: MA02). Results: In the dataset MA01 B19V genomes were detected in 42.6% of the endomyocardial biopsies (EMB) in this cohort by PCR. In the dataset MA02 comprising <i>n</i> = 638 subjects, there was no statistically significant different rate of B19V positivity in myocardial tissues comparing controls (mean: 38.8 + 24.1%) versus the MC/DCM-patients (45.5 + 24.3%; <i>p</i> = 0.58). There was also no statistical difference between the positivity rate of B19V genomes in myocardial tissues of MA01 (46.0 + 19.5%) and the two patient groups of MA02 (<i>p</i> > 0.05). Conclusions: This systematic review reveals that the mean rate of PCR detected B19V genomes in patients presenting with MC/DCM does not differ significantly from the findings in control myocardial tissues. These data imply pathogenetically insignificant latency of B19V genomes in a proportion of myocardial tissues, both in MC-/DCM-patients and in controls. More information (i.e., replicative status, viral protein expression) is pertinent to achieve a comprehensive workup of myocardial B19V infection.https://www.mdpi.com/1999-4915/11/6/566parvovirus B19B19Verythrovirusdiagnosisdilated cardiomyopathyinflammatory cardiomyopathymyocarditisprognosis |
| spellingShingle | Angelos G. Rigopoulos Bianca Klutt Marios Matiakis Athanasios Apostolou Sophie Mavrogeni Michel Noutsias Systematic Review of PCR Proof of Parvovirus B19 Genomes in Endomyocardial Biopsies of Patients Presenting with Myocarditis or Dilated Cardiomyopathy Viruses parvovirus B19 B19V erythrovirus diagnosis dilated cardiomyopathy inflammatory cardiomyopathy myocarditis prognosis |
| title | Systematic Review of PCR Proof of Parvovirus B19 Genomes in Endomyocardial Biopsies of Patients Presenting with Myocarditis or Dilated Cardiomyopathy |
| title_full | Systematic Review of PCR Proof of Parvovirus B19 Genomes in Endomyocardial Biopsies of Patients Presenting with Myocarditis or Dilated Cardiomyopathy |
| title_fullStr | Systematic Review of PCR Proof of Parvovirus B19 Genomes in Endomyocardial Biopsies of Patients Presenting with Myocarditis or Dilated Cardiomyopathy |
| title_full_unstemmed | Systematic Review of PCR Proof of Parvovirus B19 Genomes in Endomyocardial Biopsies of Patients Presenting with Myocarditis or Dilated Cardiomyopathy |
| title_short | Systematic Review of PCR Proof of Parvovirus B19 Genomes in Endomyocardial Biopsies of Patients Presenting with Myocarditis or Dilated Cardiomyopathy |
| title_sort | systematic review of pcr proof of parvovirus b19 genomes in endomyocardial biopsies of patients presenting with myocarditis or dilated cardiomyopathy |
| topic | parvovirus B19 B19V erythrovirus diagnosis dilated cardiomyopathy inflammatory cardiomyopathy myocarditis prognosis |
| url | https://www.mdpi.com/1999-4915/11/6/566 |
| work_keys_str_mv | AT angelosgrigopoulos systematicreviewofpcrproofofparvovirusb19genomesinendomyocardialbiopsiesofpatientspresentingwithmyocarditisordilatedcardiomyopathy AT biancaklutt systematicreviewofpcrproofofparvovirusb19genomesinendomyocardialbiopsiesofpatientspresentingwithmyocarditisordilatedcardiomyopathy AT mariosmatiakis systematicreviewofpcrproofofparvovirusb19genomesinendomyocardialbiopsiesofpatientspresentingwithmyocarditisordilatedcardiomyopathy AT athanasiosapostolou systematicreviewofpcrproofofparvovirusb19genomesinendomyocardialbiopsiesofpatientspresentingwithmyocarditisordilatedcardiomyopathy AT sophiemavrogeni systematicreviewofpcrproofofparvovirusb19genomesinendomyocardialbiopsiesofpatientspresentingwithmyocarditisordilatedcardiomyopathy AT michelnoutsias systematicreviewofpcrproofofparvovirusb19genomesinendomyocardialbiopsiesofpatientspresentingwithmyocarditisordilatedcardiomyopathy |