The Drosophila microRNA bantam regulates excitability in adult mushroom body output neurons to promote early night sleep
Summary: Sleep circuitry evolved to have both dedicated and context-dependent modulatory elements. Identifying modulatory subcircuits and understanding their molecular machinery is a major challenge for the sleep field. Previously, we identified 25 sleep-regulating microRNAs in Drosophila melanogast...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222011464 |
| _version_ | 1828287256349114368 |
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| author | Michael Hobin Katherine Dorfman Mohamed Adel Emmanuel J. Rivera-Rodriguez Elena A. Kuklin Dingbang Ma Leslie C. Griffith |
| author_facet | Michael Hobin Katherine Dorfman Mohamed Adel Emmanuel J. Rivera-Rodriguez Elena A. Kuklin Dingbang Ma Leslie C. Griffith |
| author_sort | Michael Hobin |
| collection | DOAJ |
| description | Summary: Sleep circuitry evolved to have both dedicated and context-dependent modulatory elements. Identifying modulatory subcircuits and understanding their molecular machinery is a major challenge for the sleep field. Previously, we identified 25 sleep-regulating microRNAs in Drosophila melanogaster, including the developmentally important microRNA bantam. Here we show that bantam acts in the adult to promote early nighttime sleep through a population of glutamatergic neurons that is intimately involved in applying contextual information to behaviors, the γ5β′2a/β′2mp/β′2mp_bilateral Mushroom Body Output Neurons (MBONs). Calcium imaging revealed that bantam inhibits the activity of these cells during the early night, but not the day. Blocking synaptic transmission in these MBONs rescued the effect of bantam knockdown. This suggests bantam promotes early night sleep via inhibition of the γ5β′2a/β′2mp/β′2mp_bilateral MBONs. RNAseq identifies Kelch and CCHamide-2 receptor as possible mediators, establishing a new role for bantam as an active regulator of sleep and neural activity in the adult fly. |
| first_indexed | 2024-04-13T09:45:38Z |
| format | Article |
| id | doaj.art-550718604ea04671a8dd0eb0dcce4fc9 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-13T09:45:38Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-550718604ea04671a8dd0eb0dcce4fc92022-12-22T02:51:46ZengElsevieriScience2589-00422022-09-01259104874The Drosophila microRNA bantam regulates excitability in adult mushroom body output neurons to promote early night sleepMichael Hobin0Katherine Dorfman1Mohamed Adel2Emmanuel J. Rivera-Rodriguez3Elena A. Kuklin4Dingbang Ma5Leslie C. Griffith6Department of Biology, Volen National Center for Complex Systems, Brandeis University, Waltham, MA 02454-9110, USADepartment of Biology, Volen National Center for Complex Systems, Brandeis University, Waltham, MA 02454-9110, USADepartment of Biology, Volen National Center for Complex Systems, Brandeis University, Waltham, MA 02454-9110, USADepartment of Biology, Volen National Center for Complex Systems, Brandeis University, Waltham, MA 02454-9110, USADepartment of Biology, Volen National Center for Complex Systems, Brandeis University, Waltham, MA 02454-9110, USADepartment of Biology, Volen National Center for Complex Systems, Brandeis University, Waltham, MA 02454-9110, USA; Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02454-9110, USADepartment of Biology, Volen National Center for Complex Systems, Brandeis University, Waltham, MA 02454-9110, USA; Corresponding authorSummary: Sleep circuitry evolved to have both dedicated and context-dependent modulatory elements. Identifying modulatory subcircuits and understanding their molecular machinery is a major challenge for the sleep field. Previously, we identified 25 sleep-regulating microRNAs in Drosophila melanogaster, including the developmentally important microRNA bantam. Here we show that bantam acts in the adult to promote early nighttime sleep through a population of glutamatergic neurons that is intimately involved in applying contextual information to behaviors, the γ5β′2a/β′2mp/β′2mp_bilateral Mushroom Body Output Neurons (MBONs). Calcium imaging revealed that bantam inhibits the activity of these cells during the early night, but not the day. Blocking synaptic transmission in these MBONs rescued the effect of bantam knockdown. This suggests bantam promotes early night sleep via inhibition of the γ5β′2a/β′2mp/β′2mp_bilateral MBONs. RNAseq identifies Kelch and CCHamide-2 receptor as possible mediators, establishing a new role for bantam as an active regulator of sleep and neural activity in the adult fly.http://www.sciencedirect.com/science/article/pii/S2589004222011464Molecular physiologyNeuroscienceCell biology |
| spellingShingle | Michael Hobin Katherine Dorfman Mohamed Adel Emmanuel J. Rivera-Rodriguez Elena A. Kuklin Dingbang Ma Leslie C. Griffith The Drosophila microRNA bantam regulates excitability in adult mushroom body output neurons to promote early night sleep iScience Molecular physiology Neuroscience Cell biology |
| title | The Drosophila microRNA bantam regulates excitability in adult mushroom body output neurons to promote early night sleep |
| title_full | The Drosophila microRNA bantam regulates excitability in adult mushroom body output neurons to promote early night sleep |
| title_fullStr | The Drosophila microRNA bantam regulates excitability in adult mushroom body output neurons to promote early night sleep |
| title_full_unstemmed | The Drosophila microRNA bantam regulates excitability in adult mushroom body output neurons to promote early night sleep |
| title_short | The Drosophila microRNA bantam regulates excitability in adult mushroom body output neurons to promote early night sleep |
| title_sort | drosophila microrna bantam regulates excitability in adult mushroom body output neurons to promote early night sleep |
| topic | Molecular physiology Neuroscience Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222011464 |
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