miRNA Expression Signatures Induced by Chicken Astrovirus Infection in Chickens
miRNAs represent ubiquitous regulators of gene expression and play an important and pivotal regulatory role in viral disease pathogenesis and virus–host interactions. Although previous studies have provided basic data for understanding the role of miRNAs in the molecular mechanisms of viral infectio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/20/15128 |
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| author | Joanna Sajewicz-Krukowska Paweł Mirosław Jan P. Jastrzębski Katarzyna Domańska-Blicharz Karolina Tarasiuk Barbara Marzec-Kotarska |
| author_facet | Joanna Sajewicz-Krukowska Paweł Mirosław Jan P. Jastrzębski Katarzyna Domańska-Blicharz Karolina Tarasiuk Barbara Marzec-Kotarska |
| author_sort | Joanna Sajewicz-Krukowska |
| collection | DOAJ |
| description | miRNAs represent ubiquitous regulators of gene expression and play an important and pivotal regulatory role in viral disease pathogenesis and virus–host interactions. Although previous studies have provided basic data for understanding the role of miRNAs in the molecular mechanisms of viral infection in birds, the role of miRNAs in the regulation of host responses to chicken astrovirus (CAstV) infection in chickens is not yet understood. In our study, we applied next-generation sequencing to profile miRNA expression in CAstV-infected chickens and to decipher miRNA-targeted specific signaling pathways engaged in potentially vital virus-infection biological processes. Among the 1354 detected miRNAs, we identified 58 mature miRNAs that were significantly differentially expressed in infected birds. Target prediction resulted in 4741 target genes. GO and KEGG pathway enrichment analyses showed that the target genes were mainly involved in the regulation of cellular processes and immune responses. |
| first_indexed | 2024-03-10T21:12:40Z |
| format | Article |
| id | doaj.art-5507784a31694b8687ccf1ebc6a2532e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T21:12:40Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5507784a31694b8687ccf1ebc6a2532e2023-11-19T16:42:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124201512810.3390/ijms242015128miRNA Expression Signatures Induced by Chicken Astrovirus Infection in ChickensJoanna Sajewicz-Krukowska0Paweł Mirosław1Jan P. Jastrzębski2Katarzyna Domańska-Blicharz3Karolina Tarasiuk4Barbara Marzec-Kotarska5Department of Poultry Diseases, National Veterinary Research Institute, 24-100 Puławy, PolandFoundation of Research and Science Development, 01-242 Warsaw, PolandDepartment of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, PolandDepartment of Poultry Diseases, National Veterinary Research Institute, 24-100 Puławy, PolandDepartment of Poultry Diseases, National Veterinary Research Institute, 24-100 Puławy, PolandDepartment of Clinical Pathomorphology, The Medical University of Lublin, 20-090 Lublin, PolandmiRNAs represent ubiquitous regulators of gene expression and play an important and pivotal regulatory role in viral disease pathogenesis and virus–host interactions. Although previous studies have provided basic data for understanding the role of miRNAs in the molecular mechanisms of viral infection in birds, the role of miRNAs in the regulation of host responses to chicken astrovirus (CAstV) infection in chickens is not yet understood. In our study, we applied next-generation sequencing to profile miRNA expression in CAstV-infected chickens and to decipher miRNA-targeted specific signaling pathways engaged in potentially vital virus-infection biological processes. Among the 1354 detected miRNAs, we identified 58 mature miRNAs that were significantly differentially expressed in infected birds. Target prediction resulted in 4741 target genes. GO and KEGG pathway enrichment analyses showed that the target genes were mainly involved in the regulation of cellular processes and immune responses.https://www.mdpi.com/1422-0067/24/20/15128chicken astroviruswhite chicks syndromemicroRNARNA-seqspleenmolecular pathogenesis |
| spellingShingle | Joanna Sajewicz-Krukowska Paweł Mirosław Jan P. Jastrzębski Katarzyna Domańska-Blicharz Karolina Tarasiuk Barbara Marzec-Kotarska miRNA Expression Signatures Induced by Chicken Astrovirus Infection in Chickens International Journal of Molecular Sciences chicken astrovirus white chicks syndrome microRNA RNA-seq spleen molecular pathogenesis |
| title | miRNA Expression Signatures Induced by Chicken Astrovirus Infection in Chickens |
| title_full | miRNA Expression Signatures Induced by Chicken Astrovirus Infection in Chickens |
| title_fullStr | miRNA Expression Signatures Induced by Chicken Astrovirus Infection in Chickens |
| title_full_unstemmed | miRNA Expression Signatures Induced by Chicken Astrovirus Infection in Chickens |
| title_short | miRNA Expression Signatures Induced by Chicken Astrovirus Infection in Chickens |
| title_sort | mirna expression signatures induced by chicken astrovirus infection in chickens |
| topic | chicken astrovirus white chicks syndrome microRNA RNA-seq spleen molecular pathogenesis |
| url | https://www.mdpi.com/1422-0067/24/20/15128 |
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