Proteomic Study Identifies Glycolytic and Inflammation Pathways Involved in Recurrent Otitis Media
Recurrent acute otitis media (RAOM) in children is clinically defined as the occurrence of at least three episodes of acute otitis media over a course of 6 months. A further common pathological condition of interest in the context of pediatric otolaryngology is adenotonsillar hypertrophy (ATH), a co...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/23/9291 |
| _version_ | 1797545580050776064 |
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| author | Blendi Ura Fulvio Celsi Luisa Zupin Giorgio Arrigoni Ilaria Battisti Bartolomea Gaita Domenico Leonardo Grasso Eva Orzan Raffaella Sagredini Egidio Barbi Sergio Crovella |
| author_facet | Blendi Ura Fulvio Celsi Luisa Zupin Giorgio Arrigoni Ilaria Battisti Bartolomea Gaita Domenico Leonardo Grasso Eva Orzan Raffaella Sagredini Egidio Barbi Sergio Crovella |
| author_sort | Blendi Ura |
| collection | DOAJ |
| description | Recurrent acute otitis media (RAOM) in children is clinically defined as the occurrence of at least three episodes of acute otitis media over a course of 6 months. A further common pathological condition of interest in the context of pediatric otolaryngology is adenotonsillar hypertrophy (ATH), a common cause of obstructive sleep apnea syndrome. Aimed at unraveling the differential modulation of proteins in the two pathologies and at understanding the possible pathways involved in their onset, we analyzed the proteomic profile of the adenoids from 14 RAOM and ATH patients by using two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS). The 2-DE coupled with MS allowed us to identify 23 spots with significant (<i>p</i>-value < 0.05) changes in protein amount, recognizing proteins involved in neutrophil degranulation and glycolysis pathways. |
| first_indexed | 2024-03-10T14:17:24Z |
| format | Article |
| id | doaj.art-5507c00d71504c13a4d490528eef733f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:17:24Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5507c00d71504c13a4d490528eef733f2023-11-20T23:38:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-12-012123929110.3390/ijms21239291Proteomic Study Identifies Glycolytic and Inflammation Pathways Involved in Recurrent Otitis MediaBlendi Ura0Fulvio Celsi1Luisa Zupin2Giorgio Arrigoni3Ilaria Battisti4Bartolomea Gaita5Domenico Leonardo Grasso6Eva Orzan7Raffaella Sagredini8Egidio Barbi9Sergio Crovella10Institute for Maternal and Child Health–IRCCS “Burlo Garofolo”, 65/1 Via dell’Istria, 34137 Trieste, ItalyInstitute for Maternal and Child Health–IRCCS “Burlo Garofolo”, 65/1 Via dell’Istria, 34137 Trieste, ItalyInstitute for Maternal and Child Health–IRCCS “Burlo Garofolo”, 65/1 Via dell’Istria, 34137 Trieste, ItalyDepartment of Biomedical Sciences, University of Padova, Via U. Bassi 58/B, 35121 Padova, ItalyDepartment of Biomedical Sciences, University of Padova, Via U. Bassi 58/B, 35121 Padova, ItalyInstitute for Maternal and Child Health–IRCCS “Burlo Garofolo”, 65/1 Via dell’Istria, 34137 Trieste, ItalyInstitute for Maternal and Child Health–IRCCS “Burlo Garofolo”, 65/1 Via dell’Istria, 34137 Trieste, ItalyInstitute for Maternal and Child Health–IRCCS “Burlo Garofolo”, 65/1 Via dell’Istria, 34137 Trieste, ItalyInstitute for Maternal and Child Health–IRCCS “Burlo Garofolo”, 65/1 Via dell’Istria, 34137 Trieste, ItalyInstitute for Maternal and Child Health–IRCCS “Burlo Garofolo”, 65/1 Via dell’Istria, 34137 Trieste, ItalyDepartment of Biological and Environmental Sciences, College of Arts and Sciences, Qatar University—Women′s College of Sciences Building, Doha 2713, QatarRecurrent acute otitis media (RAOM) in children is clinically defined as the occurrence of at least three episodes of acute otitis media over a course of 6 months. A further common pathological condition of interest in the context of pediatric otolaryngology is adenotonsillar hypertrophy (ATH), a common cause of obstructive sleep apnea syndrome. Aimed at unraveling the differential modulation of proteins in the two pathologies and at understanding the possible pathways involved in their onset, we analyzed the proteomic profile of the adenoids from 14 RAOM and ATH patients by using two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS). The 2-DE coupled with MS allowed us to identify 23 spots with significant (<i>p</i>-value < 0.05) changes in protein amount, recognizing proteins involved in neutrophil degranulation and glycolysis pathways.https://www.mdpi.com/1422-0067/21/23/9291recurrent otitisadenotonsillar hypertrophy2-DEproteomics |
| spellingShingle | Blendi Ura Fulvio Celsi Luisa Zupin Giorgio Arrigoni Ilaria Battisti Bartolomea Gaita Domenico Leonardo Grasso Eva Orzan Raffaella Sagredini Egidio Barbi Sergio Crovella Proteomic Study Identifies Glycolytic and Inflammation Pathways Involved in Recurrent Otitis Media International Journal of Molecular Sciences recurrent otitis adenotonsillar hypertrophy 2-DE proteomics |
| title | Proteomic Study Identifies Glycolytic and Inflammation Pathways Involved in Recurrent Otitis Media |
| title_full | Proteomic Study Identifies Glycolytic and Inflammation Pathways Involved in Recurrent Otitis Media |
| title_fullStr | Proteomic Study Identifies Glycolytic and Inflammation Pathways Involved in Recurrent Otitis Media |
| title_full_unstemmed | Proteomic Study Identifies Glycolytic and Inflammation Pathways Involved in Recurrent Otitis Media |
| title_short | Proteomic Study Identifies Glycolytic and Inflammation Pathways Involved in Recurrent Otitis Media |
| title_sort | proteomic study identifies glycolytic and inflammation pathways involved in recurrent otitis media |
| topic | recurrent otitis adenotonsillar hypertrophy 2-DE proteomics |
| url | https://www.mdpi.com/1422-0067/21/23/9291 |
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