Quantitative super-resolution microscopy reveals the differences in the nanoscale distribution of nuclear phosphatidylinositol 4,5-bisphosphate in human healthy skin and skin warts
Introduction: Imaging of human clinical formalin-fixed paraffin-embedded (FFPE) tissue sections provides insights into healthy and diseased states and therefore represents a valuable resource for basic research, as well as for diagnostic and clinical purposes. However, conventional light microscopy...
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2023.1217637/full |
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| author | Peter Hoboth Martin Sztacho Alexander Quaas Baki Akgül Pavel Hozák Pavel Hozák |
| author_facet | Peter Hoboth Martin Sztacho Alexander Quaas Baki Akgül Pavel Hozák Pavel Hozák |
| author_sort | Peter Hoboth |
| collection | DOAJ |
| description | Introduction: Imaging of human clinical formalin-fixed paraffin-embedded (FFPE) tissue sections provides insights into healthy and diseased states and therefore represents a valuable resource for basic research, as well as for diagnostic and clinical purposes. However, conventional light microscopy does not allow to observe the molecular details of tissue and cell architecture due to the diffraction limit of light. Super-resolution microscopy overcomes this limitation and provides access to the nanoscale details of tissue and cell organization.Methods: Here, we used quantitative multicolor stimulated emission depletion (STED) nanoscopy to study the nanoscale distribution of the nuclear phosphatidylinositol 4,5-bisphosphate (nPI(4,5)P2) with respect to the nuclear speckles (NS) marker SON.Results: Increased nPI(4,5)P2 signals were previously linked to human papillomavirus (HPV)-mediated carcinogenesis, while NS-associated PI(4,5)P2 represents the largest pool of nPI(4,5)P2 visualized by staining and microscopy. The implementation of multicolor STED nanoscopy in human clinical FFPE skin and wart sections allowed us to provide here the quantitative evidence for higher levels of NS-associated PI(4,5)P2 in HPV-induced warts compared to control skin.Discussion: These data expand the previous reports of HPV-induced increase of nPI(4,5)P2 levels and reveal for the first time the functional, tissue-specific localization of nPI(4,5)P2 within NS in clinically relevant samples. Moreover, our approach is widely applicable to other human clinical FFPE tissues as an informative addition to the classical histochemistry. |
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| id | doaj.art-5508c7f721834762a759861ee4f41d39 |
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| issn | 2296-634X |
| language | English |
| last_indexed | 2024-03-13T00:51:12Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-5508c7f721834762a759861ee4f41d392023-07-07T13:54:28ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2023-07-011110.3389/fcell.2023.12176371217637Quantitative super-resolution microscopy reveals the differences in the nanoscale distribution of nuclear phosphatidylinositol 4,5-bisphosphate in human healthy skin and skin wartsPeter Hoboth0Martin Sztacho1Alexander Quaas2Baki Akgül3Pavel Hozák4Pavel Hozák5Department of Biology of the Cell Nucleus, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, CzechiaDepartment of Biology of the Cell Nucleus, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, CzechiaInstitute of Pathology, Medical Faculty and University Hospital Cologne, Cologne, GermanyInstitute of Virology, University of Cologne, Medical Faculty and University Hospital Cologne, Cologne, GermanyDepartment of Biology of the Cell Nucleus, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, CzechiaMicroscopy Centre, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, CzechiaIntroduction: Imaging of human clinical formalin-fixed paraffin-embedded (FFPE) tissue sections provides insights into healthy and diseased states and therefore represents a valuable resource for basic research, as well as for diagnostic and clinical purposes. However, conventional light microscopy does not allow to observe the molecular details of tissue and cell architecture due to the diffraction limit of light. Super-resolution microscopy overcomes this limitation and provides access to the nanoscale details of tissue and cell organization.Methods: Here, we used quantitative multicolor stimulated emission depletion (STED) nanoscopy to study the nanoscale distribution of the nuclear phosphatidylinositol 4,5-bisphosphate (nPI(4,5)P2) with respect to the nuclear speckles (NS) marker SON.Results: Increased nPI(4,5)P2 signals were previously linked to human papillomavirus (HPV)-mediated carcinogenesis, while NS-associated PI(4,5)P2 represents the largest pool of nPI(4,5)P2 visualized by staining and microscopy. The implementation of multicolor STED nanoscopy in human clinical FFPE skin and wart sections allowed us to provide here the quantitative evidence for higher levels of NS-associated PI(4,5)P2 in HPV-induced warts compared to control skin.Discussion: These data expand the previous reports of HPV-induced increase of nPI(4,5)P2 levels and reveal for the first time the functional, tissue-specific localization of nPI(4,5)P2 within NS in clinically relevant samples. Moreover, our approach is widely applicable to other human clinical FFPE tissues as an informative addition to the classical histochemistry.https://www.frontiersin.org/articles/10.3389/fcell.2023.1217637/fullformalin-fixed paraffin-embedded tissue sectionsSTED nanoscopycell nucleushuman papillomavirus (HPV)nuclear architecturephosphatidylinositol 4,5-bisphosphate |
| spellingShingle | Peter Hoboth Martin Sztacho Alexander Quaas Baki Akgül Pavel Hozák Pavel Hozák Quantitative super-resolution microscopy reveals the differences in the nanoscale distribution of nuclear phosphatidylinositol 4,5-bisphosphate in human healthy skin and skin warts Frontiers in Cell and Developmental Biology formalin-fixed paraffin-embedded tissue sections STED nanoscopy cell nucleus human papillomavirus (HPV) nuclear architecture phosphatidylinositol 4,5-bisphosphate |
| title | Quantitative super-resolution microscopy reveals the differences in the nanoscale distribution of nuclear phosphatidylinositol 4,5-bisphosphate in human healthy skin and skin warts |
| title_full | Quantitative super-resolution microscopy reveals the differences in the nanoscale distribution of nuclear phosphatidylinositol 4,5-bisphosphate in human healthy skin and skin warts |
| title_fullStr | Quantitative super-resolution microscopy reveals the differences in the nanoscale distribution of nuclear phosphatidylinositol 4,5-bisphosphate in human healthy skin and skin warts |
| title_full_unstemmed | Quantitative super-resolution microscopy reveals the differences in the nanoscale distribution of nuclear phosphatidylinositol 4,5-bisphosphate in human healthy skin and skin warts |
| title_short | Quantitative super-resolution microscopy reveals the differences in the nanoscale distribution of nuclear phosphatidylinositol 4,5-bisphosphate in human healthy skin and skin warts |
| title_sort | quantitative super resolution microscopy reveals the differences in the nanoscale distribution of nuclear phosphatidylinositol 4 5 bisphosphate in human healthy skin and skin warts |
| topic | formalin-fixed paraffin-embedded tissue sections STED nanoscopy cell nucleus human papillomavirus (HPV) nuclear architecture phosphatidylinositol 4,5-bisphosphate |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2023.1217637/full |
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