Research Note: Preliminary results, first detection of Enterococcus cecorum from environmental samples by streaking on X-Gluc containing selective media
ABSTRACT: The isolation of cultivable E. cecorum from the environment of poultry houses remains a challenge. Environmental samples (dust wipes, equipment swabs, pooled feces) and samples from culled bird vertebras were collected from an infected broiler flock on d 37 posthatching. To isolate the bac...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-01-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123007721 |
| _version_ | 1797366653433937920 |
|---|---|
| author | Jesper Tessin Judith Rohde Arne Jung Nicole Kemper Jochen Schulz |
| author_facet | Jesper Tessin Judith Rohde Arne Jung Nicole Kemper Jochen Schulz |
| author_sort | Jesper Tessin |
| collection | DOAJ |
| description | ABSTRACT: The isolation of cultivable E. cecorum from the environment of poultry houses remains a challenge. Environmental samples (dust wipes, equipment swabs, pooled feces) and samples from culled bird vertebras were collected from an infected broiler flock on d 37 posthatching. To isolate the bacterium from the cultivable microbiota, suspensions from the environmental samples were streaked onto a blood agar base medium supplemented with 5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid cyclohexylammonium salt (X-Gluc), colistin sulfate, and nalidixin. The chromogenic reaction facilitated the isolation of E. cecorum from contaminated surfaces and pooled feces. Isolates from both the environment and vertebras were confirmed using MALDI-TOF and PCR analysis. Colony appearance and antimicrobial susceptibility tests revealed no phenotypic differences among the isolates. It remained unclear whether the isolates originated from the same clone. However, the principle of isolating the pathogen by streaking on a chromogenic agar may motivate researchers to investigate the transmission routes of infectious isolates, potentially leading to the optimization of biosecurity measures. |
| first_indexed | 2024-03-08T17:07:40Z |
| format | Article |
| id | doaj.art-550962c1007b41d1949216bf9c294fff |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-08T17:07:40Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-550962c1007b41d1949216bf9c294fff2024-01-04T04:35:45ZengElsevierPoultry Science0032-57912024-01-011031103253Research Note: Preliminary results, first detection of Enterococcus cecorum from environmental samples by streaking on X-Gluc containing selective mediaJesper Tessin0Judith Rohde1Arne Jung2Nicole Kemper3Jochen Schulz4Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, 30173 Hannover, GermanyInstitute for Microbiology, University of Veterinary Medicine Hannover, 30173 Hannover, GermanyClinic for Poultry, University of Veterinary Medicine Hannover, 30559 Hannover, GermanyInstitute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, 30173 Hannover, GermanyInstitute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, 30173 Hannover, Germany; Corresponding author:ABSTRACT: The isolation of cultivable E. cecorum from the environment of poultry houses remains a challenge. Environmental samples (dust wipes, equipment swabs, pooled feces) and samples from culled bird vertebras were collected from an infected broiler flock on d 37 posthatching. To isolate the bacterium from the cultivable microbiota, suspensions from the environmental samples were streaked onto a blood agar base medium supplemented with 5-bromo-4-chloro-3-indolyl-beta-D-glucuronic acid cyclohexylammonium salt (X-Gluc), colistin sulfate, and nalidixin. The chromogenic reaction facilitated the isolation of E. cecorum from contaminated surfaces and pooled feces. Isolates from both the environment and vertebras were confirmed using MALDI-TOF and PCR analysis. Colony appearance and antimicrobial susceptibility tests revealed no phenotypic differences among the isolates. It remained unclear whether the isolates originated from the same clone. However, the principle of isolating the pathogen by streaking on a chromogenic agar may motivate researchers to investigate the transmission routes of infectious isolates, potentially leading to the optimization of biosecurity measures.http://www.sciencedirect.com/science/article/pii/S0032579123007721Enterococcus cecorumdetectionenvironmentX-Glucdirect streaking |
| spellingShingle | Jesper Tessin Judith Rohde Arne Jung Nicole Kemper Jochen Schulz Research Note: Preliminary results, first detection of Enterococcus cecorum from environmental samples by streaking on X-Gluc containing selective media Poultry Science Enterococcus cecorum detection environment X-Gluc direct streaking |
| title | Research Note: Preliminary results, first detection of Enterococcus cecorum from environmental samples by streaking on X-Gluc containing selective media |
| title_full | Research Note: Preliminary results, first detection of Enterococcus cecorum from environmental samples by streaking on X-Gluc containing selective media |
| title_fullStr | Research Note: Preliminary results, first detection of Enterococcus cecorum from environmental samples by streaking on X-Gluc containing selective media |
| title_full_unstemmed | Research Note: Preliminary results, first detection of Enterococcus cecorum from environmental samples by streaking on X-Gluc containing selective media |
| title_short | Research Note: Preliminary results, first detection of Enterococcus cecorum from environmental samples by streaking on X-Gluc containing selective media |
| title_sort | research note preliminary results first detection of enterococcus cecorum from environmental samples by streaking on x gluc containing selective media |
| topic | Enterococcus cecorum detection environment X-Gluc direct streaking |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123007721 |
| work_keys_str_mv | AT jespertessin researchnotepreliminaryresultsfirstdetectionofenterococcuscecorumfromenvironmentalsamplesbystreakingonxgluccontainingselectivemedia AT judithrohde researchnotepreliminaryresultsfirstdetectionofenterococcuscecorumfromenvironmentalsamplesbystreakingonxgluccontainingselectivemedia AT arnejung researchnotepreliminaryresultsfirstdetectionofenterococcuscecorumfromenvironmentalsamplesbystreakingonxgluccontainingselectivemedia AT nicolekemper researchnotepreliminaryresultsfirstdetectionofenterococcuscecorumfromenvironmentalsamplesbystreakingonxgluccontainingselectivemedia AT jochenschulz researchnotepreliminaryresultsfirstdetectionofenterococcuscecorumfromenvironmentalsamplesbystreakingonxgluccontainingselectivemedia |