The Pathogen-Induced MATE Gene <i>TaPIMA1</i> Is Required for Defense Responses to <i>Rhizoctonia cerealis</i> in Wheat
The sharp eyespot, mainly caused by the soil-borne fungus <i>Rhizoctonia cerealis</i>, is a devastating disease endangering production of wheat (<i>Triticum aestivum</i>). Multi-Antimicrobial Extrusion (MATE) family genes are widely distributed in plant species, but little is...
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MDPI AG
2022-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/6/3377 |
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| author | Qiang Su Wei Rong Zengyan Zhang |
| author_facet | Qiang Su Wei Rong Zengyan Zhang |
| author_sort | Qiang Su |
| collection | DOAJ |
| description | The sharp eyespot, mainly caused by the soil-borne fungus <i>Rhizoctonia cerealis</i>, is a devastating disease endangering production of wheat (<i>Triticum aestivum</i>). Multi-Antimicrobial Extrusion (MATE) family genes are widely distributed in plant species, but little is known about MATE functions in wheat disease resistance. In this study, we identified <i>TaPIMA1</i>, a pathogen-induced MATE gene in wheat, from RNA-seq data. <i>TaPIMA1</i> expression was induced by <i>Rhizoctonia cerealis</i> and was higher in sharp eyespot-resistant wheat genotypes than in susceptible wheat genotypes. Molecular biology assays showed that TaPIMA1 belonged to the MATE family, and the expressed protein could distribute in the cytoplasm and plasma membrane. Virus-Induced Gene Silencing plus disease assessment indicated that knock-down of <i>TaPIMA1</i> impaired resistance of wheat to sharp eyespot and down-regulated the expression of defense genes (<i>Defensin</i>, <i>PR10</i>, <i>PR1.2</i>, and <i>Chitinase3</i>). Furthermore, <i>TaPIMA1</i> was rapidly induced by exogenous H<sub>2</sub>O<sub>2</sub> and jasmonate (JA) treatments, which also promoted the expression of pathogenesis-related genes. These results suggested that <i>TaPIMA1</i> might positively regulate the defense against <i>R. cerealis</i> by up-regulating the expression of defense-associated genes in H<sub>2</sub>O<sub>2</sub> and JA signal pathways. This study sheds light on the role of MATE transporter in wheat defense to <i>Rhizoctonia cerealis</i> and provides a potential gene for improving wheat resistance against sharp eyespot. |
| first_indexed | 2024-03-09T19:41:18Z |
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| language | English |
| last_indexed | 2024-03-09T19:41:18Z |
| publishDate | 2022-03-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-550a7fea8a5c4a0f9041934ed06148e92023-11-24T01:38:28ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-03-01236337710.3390/ijms23063377The Pathogen-Induced MATE Gene <i>TaPIMA1</i> Is Required for Defense Responses to <i>Rhizoctonia cerealis</i> in WheatQiang Su0Wei Rong1Zengyan Zhang2The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaThe National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaThe National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaThe sharp eyespot, mainly caused by the soil-borne fungus <i>Rhizoctonia cerealis</i>, is a devastating disease endangering production of wheat (<i>Triticum aestivum</i>). Multi-Antimicrobial Extrusion (MATE) family genes are widely distributed in plant species, but little is known about MATE functions in wheat disease resistance. In this study, we identified <i>TaPIMA1</i>, a pathogen-induced MATE gene in wheat, from RNA-seq data. <i>TaPIMA1</i> expression was induced by <i>Rhizoctonia cerealis</i> and was higher in sharp eyespot-resistant wheat genotypes than in susceptible wheat genotypes. Molecular biology assays showed that TaPIMA1 belonged to the MATE family, and the expressed protein could distribute in the cytoplasm and plasma membrane. Virus-Induced Gene Silencing plus disease assessment indicated that knock-down of <i>TaPIMA1</i> impaired resistance of wheat to sharp eyespot and down-regulated the expression of defense genes (<i>Defensin</i>, <i>PR10</i>, <i>PR1.2</i>, and <i>Chitinase3</i>). Furthermore, <i>TaPIMA1</i> was rapidly induced by exogenous H<sub>2</sub>O<sub>2</sub> and jasmonate (JA) treatments, which also promoted the expression of pathogenesis-related genes. These results suggested that <i>TaPIMA1</i> might positively regulate the defense against <i>R. cerealis</i> by up-regulating the expression of defense-associated genes in H<sub>2</sub>O<sub>2</sub> and JA signal pathways. This study sheds light on the role of MATE transporter in wheat defense to <i>Rhizoctonia cerealis</i> and provides a potential gene for improving wheat resistance against sharp eyespot.https://www.mdpi.com/1422-0067/23/6/3377wheat (<i>Triticum aestivum</i>)defense<i>Rhizoctonia cerealis</i>multi-antimicrobial extrusion family<i>TaPIMA1</i> |
| spellingShingle | Qiang Su Wei Rong Zengyan Zhang The Pathogen-Induced MATE Gene <i>TaPIMA1</i> Is Required for Defense Responses to <i>Rhizoctonia cerealis</i> in Wheat International Journal of Molecular Sciences wheat (<i>Triticum aestivum</i>) defense <i>Rhizoctonia cerealis</i> multi-antimicrobial extrusion family <i>TaPIMA1</i> |
| title | The Pathogen-Induced MATE Gene <i>TaPIMA1</i> Is Required for Defense Responses to <i>Rhizoctonia cerealis</i> in Wheat |
| title_full | The Pathogen-Induced MATE Gene <i>TaPIMA1</i> Is Required for Defense Responses to <i>Rhizoctonia cerealis</i> in Wheat |
| title_fullStr | The Pathogen-Induced MATE Gene <i>TaPIMA1</i> Is Required for Defense Responses to <i>Rhizoctonia cerealis</i> in Wheat |
| title_full_unstemmed | The Pathogen-Induced MATE Gene <i>TaPIMA1</i> Is Required for Defense Responses to <i>Rhizoctonia cerealis</i> in Wheat |
| title_short | The Pathogen-Induced MATE Gene <i>TaPIMA1</i> Is Required for Defense Responses to <i>Rhizoctonia cerealis</i> in Wheat |
| title_sort | pathogen induced mate gene i tapima1 i is required for defense responses to i rhizoctonia cerealis i in wheat |
| topic | wheat (<i>Triticum aestivum</i>) defense <i>Rhizoctonia cerealis</i> multi-antimicrobial extrusion family <i>TaPIMA1</i> |
| url | https://www.mdpi.com/1422-0067/23/6/3377 |
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