| Summary: | Bio-succinic acid production using microorganisms has been interesting as an environmentally friendly process. Palm oil mill effluent (POME) was considered as a cheap substrate to lower the cost of production. It was revealed that 2-fold diluted POME produced more succinic acid than undiluted and 5-fold diluted POME. In addition, the effects of various neutralizing agents on succinic acid production utilized to manage pH and CO<sub>2</sub> supply indicated that the utilization of MgCO<sub>3</sub> as a neutralizing agent produced succinic acid of 11.5 g/L with a small amount of by-product synthesis. Plackett–Burman Design (PBD) was used to screen the most significant nutrients for bio-succinic acid production from 2-fold diluted POME using <i>E. gallinarum.</i> From the Pareto chart, MgCO<sub>3</sub> and peptone presented the highest positive effect on the production of succinic acid. In addition, Box–Behnken Design (BBD) was conducted to increase bio-succinic acid production. Experiments showed the highest production of succinic acid of 23.7 g/L with the addition of 22.5 g/L MgCO<sub>3</sub> and 12.0 g/L peptone in 2-fold diluted POME. Moreover, the experiment of replacing MgCO<sub>3</sub> with CO<sub>2</sub> from biogas resulted in 19.1 g/L of succinic acid, simultaneously creating the high purity of biogas and a higher CH<sub>4</sub> content.
|
|---|