Bio-Succinic Acid Production from Palm Oil Mill Effluent Using <i>Enterococcus gallinarum</i> with Sequential Purification of Biogas
Bio-succinic acid production using microorganisms has been interesting as an environmentally friendly process. Palm oil mill effluent (POME) was considered as a cheap substrate to lower the cost of production. It was revealed that 2-fold diluted POME produced more succinic acid than undiluted and 5-...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/4/369 |
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| author | Pooja Vilas Nagime Apichat Upaichit Benjamas Cheirsilp Piyarat Boonsawang |
| author_facet | Pooja Vilas Nagime Apichat Upaichit Benjamas Cheirsilp Piyarat Boonsawang |
| author_sort | Pooja Vilas Nagime |
| collection | DOAJ |
| description | Bio-succinic acid production using microorganisms has been interesting as an environmentally friendly process. Palm oil mill effluent (POME) was considered as a cheap substrate to lower the cost of production. It was revealed that 2-fold diluted POME produced more succinic acid than undiluted and 5-fold diluted POME. In addition, the effects of various neutralizing agents on succinic acid production utilized to manage pH and CO<sub>2</sub> supply indicated that the utilization of MgCO<sub>3</sub> as a neutralizing agent produced succinic acid of 11.5 g/L with a small amount of by-product synthesis. Plackett–Burman Design (PBD) was used to screen the most significant nutrients for bio-succinic acid production from 2-fold diluted POME using <i>E. gallinarum.</i> From the Pareto chart, MgCO<sub>3</sub> and peptone presented the highest positive effect on the production of succinic acid. In addition, Box–Behnken Design (BBD) was conducted to increase bio-succinic acid production. Experiments showed the highest production of succinic acid of 23.7 g/L with the addition of 22.5 g/L MgCO<sub>3</sub> and 12.0 g/L peptone in 2-fold diluted POME. Moreover, the experiment of replacing MgCO<sub>3</sub> with CO<sub>2</sub> from biogas resulted in 19.1 g/L of succinic acid, simultaneously creating the high purity of biogas and a higher CH<sub>4</sub> content. |
| first_indexed | 2024-03-11T05:02:01Z |
| format | Article |
| id | doaj.art-550a8f6e6375499d83267b9b15a468f4 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T05:02:01Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-550a8f6e6375499d83267b9b15a468f42023-11-17T19:10:56ZengMDPI AGFermentation2311-56372023-04-019436910.3390/fermentation9040369Bio-Succinic Acid Production from Palm Oil Mill Effluent Using <i>Enterococcus gallinarum</i> with Sequential Purification of BiogasPooja Vilas Nagime0Apichat Upaichit1Benjamas Cheirsilp2Piyarat Boonsawang3Center of Excellence in Innovative Biotechnology for Sustainable Utilization of Bioresources, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai 90110, ThailandCenter of Excellence in Innovative Biotechnology for Sustainable Utilization of Bioresources, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai 90110, ThailandCenter of Excellence in Innovative Biotechnology for Sustainable Utilization of Bioresources, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai 90110, ThailandCenter of Excellence in Innovative Biotechnology for Sustainable Utilization of Bioresources, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai 90110, ThailandBio-succinic acid production using microorganisms has been interesting as an environmentally friendly process. Palm oil mill effluent (POME) was considered as a cheap substrate to lower the cost of production. It was revealed that 2-fold diluted POME produced more succinic acid than undiluted and 5-fold diluted POME. In addition, the effects of various neutralizing agents on succinic acid production utilized to manage pH and CO<sub>2</sub> supply indicated that the utilization of MgCO<sub>3</sub> as a neutralizing agent produced succinic acid of 11.5 g/L with a small amount of by-product synthesis. Plackett–Burman Design (PBD) was used to screen the most significant nutrients for bio-succinic acid production from 2-fold diluted POME using <i>E. gallinarum.</i> From the Pareto chart, MgCO<sub>3</sub> and peptone presented the highest positive effect on the production of succinic acid. In addition, Box–Behnken Design (BBD) was conducted to increase bio-succinic acid production. Experiments showed the highest production of succinic acid of 23.7 g/L with the addition of 22.5 g/L MgCO<sub>3</sub> and 12.0 g/L peptone in 2-fold diluted POME. Moreover, the experiment of replacing MgCO<sub>3</sub> with CO<sub>2</sub> from biogas resulted in 19.1 g/L of succinic acid, simultaneously creating the high purity of biogas and a higher CH<sub>4</sub> content.https://www.mdpi.com/2311-5637/9/4/369succinic acidpalm oil wastewaterneutralizing agentsPlackett–Burman designBox–Behnken design |
| spellingShingle | Pooja Vilas Nagime Apichat Upaichit Benjamas Cheirsilp Piyarat Boonsawang Bio-Succinic Acid Production from Palm Oil Mill Effluent Using <i>Enterococcus gallinarum</i> with Sequential Purification of Biogas Fermentation succinic acid palm oil wastewater neutralizing agents Plackett–Burman design Box–Behnken design |
| title | Bio-Succinic Acid Production from Palm Oil Mill Effluent Using <i>Enterococcus gallinarum</i> with Sequential Purification of Biogas |
| title_full | Bio-Succinic Acid Production from Palm Oil Mill Effluent Using <i>Enterococcus gallinarum</i> with Sequential Purification of Biogas |
| title_fullStr | Bio-Succinic Acid Production from Palm Oil Mill Effluent Using <i>Enterococcus gallinarum</i> with Sequential Purification of Biogas |
| title_full_unstemmed | Bio-Succinic Acid Production from Palm Oil Mill Effluent Using <i>Enterococcus gallinarum</i> with Sequential Purification of Biogas |
| title_short | Bio-Succinic Acid Production from Palm Oil Mill Effluent Using <i>Enterococcus gallinarum</i> with Sequential Purification of Biogas |
| title_sort | bio succinic acid production from palm oil mill effluent using i enterococcus gallinarum i with sequential purification of biogas |
| topic | succinic acid palm oil wastewater neutralizing agents Plackett–Burman design Box–Behnken design |
| url | https://www.mdpi.com/2311-5637/9/4/369 |
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