High expression of Ku70 promotes proliferation of ovarian cancer cell lines and is associated with a poor prognosis for patients
Objective To investigate the expression of X-ray-repaired cross complementary protein 6 (Ku70/XRCC6) in epithelial ovarian cancer(EOC) and its effects on the proliferation, apoptosis and migration of ovarian cancer cells. Methods Western blot was used to detect the expression of Ku70 in 9 ovarian ca...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2022-09-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-42-9-1391.pdf |
| _version_ | 1797365850267713536 |
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| author | WANG Juan, ZHANG Xiao-yan, ZHU Ai-hua, YU Hong-bo, FAN Xin-dan, TANG Chun-hui, YAO Xiu-fang |
| author_facet | WANG Juan, ZHANG Xiao-yan, ZHU Ai-hua, YU Hong-bo, FAN Xin-dan, TANG Chun-hui, YAO Xiu-fang |
| author_sort | WANG Juan, ZHANG Xiao-yan, ZHU Ai-hua, YU Hong-bo, FAN Xin-dan, TANG Chun-hui, YAO Xiu-fang |
| collection | DOAJ |
| description | Objective To investigate the expression of X-ray-repaired cross complementary protein 6 (Ku70/XRCC6) in epithelial ovarian cancer(EOC) and its effects on the proliferation, apoptosis and migration of ovarian cancer cells. Methods Western blot was used to detect the expression of Ku70 in 9 ovarian cancer tissues of different grades and 3 normal ovarian tissues. Immunohistochemistry was used to detect the expression of Ku70 in 119 ovarian cancer tissues, and the relationship between Ku70 expression and clinicopathological parameters was analyzed. Kaplan-meier survival curve was used to analyze the relationship between Ku70 expression and 5-year survival. Colony formation and CCK-8 assay were used to analyze the effect of Ku70 expression on proliferation of ovarian cancer cells, and flow cytometry was used to detect the effect of Ku70 expression on apoptosis of ovarian cancer cells. The effect of Ku70 expression on ovarian cancer cell migration was analyzed by scratch test and Transwell chamber method. Results The expression of Ku70 in ovarian cancer tissues was higher than that in normal ovarian tissues. The expression of Ku70 was closely correlated with pathological features (P＜0.05). The survival rate of ovarian cancer patients with high Ku70 expression was lower than that of patients with low Ku70 expression(P＜0.05). Inhibiting the expression of Ku70 decreased poliferation and migration, and increased apoptosis of ovarian cancer cells (P＜0.05). Conclusions The abnormal expression of Ku70 is closely related to the occurrence of ovarian cancer and so Ku70 is a potential therapeutic target for ovarian cancer treatment. |
| first_indexed | 2024-03-08T16:54:52Z |
| format | Article |
| id | doaj.art-550b1fc52dd94ca1a27f2432315e0967 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:54:52Z |
| publishDate | 2022-09-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-550b1fc52dd94ca1a27f2432315e09672024-01-05T02:36:18ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252022-09-014291391139910.16352/j.issn.1001-6325.2022.09.1391High expression of Ku70 promotes proliferation of ovarian cancer cell lines and is associated with a poor prognosis for patientsWANG Juan, ZHANG Xiao-yan, ZHU Ai-hua, YU Hong-bo, FAN Xin-dan, TANG Chun-hui, YAO Xiu-fang01. Department of Obstetrics and Gynecology, the Affiliated Maternal and child Health Hospital of Nantong University, Nantong 226001;;2. Department of Obstetrics and Gynecology, the Affiliated Rudong Hospital of Nantong University, Nantong 226400;;4. Department of Pathology, the Affiliated Rudong Hospital of Nantong University, Nantong 226400;;3. Department of Obstetrics and Gynecology, the Affiliated Hospital of Nantong University, Nantong 226001, ChinaObjective To investigate the expression of X-ray-repaired cross complementary protein 6 (Ku70/XRCC6) in epithelial ovarian cancer(EOC) and its effects on the proliferation, apoptosis and migration of ovarian cancer cells. Methods Western blot was used to detect the expression of Ku70 in 9 ovarian cancer tissues of different grades and 3 normal ovarian tissues. Immunohistochemistry was used to detect the expression of Ku70 in 119 ovarian cancer tissues, and the relationship between Ku70 expression and clinicopathological parameters was analyzed. Kaplan-meier survival curve was used to analyze the relationship between Ku70 expression and 5-year survival. Colony formation and CCK-8 assay were used to analyze the effect of Ku70 expression on proliferation of ovarian cancer cells, and flow cytometry was used to detect the effect of Ku70 expression on apoptosis of ovarian cancer cells. The effect of Ku70 expression on ovarian cancer cell migration was analyzed by scratch test and Transwell chamber method. Results The expression of Ku70 in ovarian cancer tissues was higher than that in normal ovarian tissues. The expression of Ku70 was closely correlated with pathological features (P＜0.05). The survival rate of ovarian cancer patients with high Ku70 expression was lower than that of patients with low Ku70 expression(P＜0.05). Inhibiting the expression of Ku70 decreased poliferation and migration, and increased apoptosis of ovarian cancer cells (P＜0.05). Conclusions The abnormal expression of Ku70 is closely related to the occurrence of ovarian cancer and so Ku70 is a potential therapeutic target for ovarian cancer treatment.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-42-9-1391.pdfku70/xrcc6| epithelial ovarian cancer| proliferation| apoptosis| migration |
| spellingShingle | WANG Juan, ZHANG Xiao-yan, ZHU Ai-hua, YU Hong-bo, FAN Xin-dan, TANG Chun-hui, YAO Xiu-fang High expression of Ku70 promotes proliferation of ovarian cancer cell lines and is associated with a poor prognosis for patients Jichu yixue yu linchuang ku70/xrcc6| epithelial ovarian cancer| proliferation| apoptosis| migration |
| title | High expression of Ku70 promotes proliferation of ovarian cancer cell lines and is associated with a poor prognosis for patients |
| title_full | High expression of Ku70 promotes proliferation of ovarian cancer cell lines and is associated with a poor prognosis for patients |
| title_fullStr | High expression of Ku70 promotes proliferation of ovarian cancer cell lines and is associated with a poor prognosis for patients |
| title_full_unstemmed | High expression of Ku70 promotes proliferation of ovarian cancer cell lines and is associated with a poor prognosis for patients |
| title_short | High expression of Ku70 promotes proliferation of ovarian cancer cell lines and is associated with a poor prognosis for patients |
| title_sort | high expression of ku70 promotes proliferation of ovarian cancer cell lines and is associated with a poor prognosis for patients |
| topic | ku70/xrcc6| epithelial ovarian cancer| proliferation| apoptosis| migration |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-42-9-1391.pdf |
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