Comparative effects of balm hydro alcoholic extract and diazepam on reducing anxiety of in mice
Background & aim: Anxiety is a normal feeling which is experienced in threatening situations and can affect neural system. Wide spread of anxiety forces many people to permanent use of anti-stress drugs especially benzodiazepines. This study was carried out to compare the effects of balm extract...
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| Format: | Article |
| Language: | fas |
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Yasuj University Of Medical Sciences
2016-01-01
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| Series: | Armaghane Danesh Bimonthly Journal |
| Subjects: | |
| Online Access: | http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-300-1&slc_lang=en&sid=1 |
| _version_ | 1811236688382394368 |
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| author | M Modaresi M Basravi I Sajadia |
| author_facet | M Modaresi M Basravi I Sajadia |
| author_sort | M Modaresi |
| collection | DOAJ |
| description | Background & aim: Anxiety is a normal feeling which is experienced in threatening situations and can affect neural system. Wide spread of anxiety forces many people to permanent use of anti-stress drugs especially benzodiazepines. This study was carried out to compare the effects of balm extract and diazepam on anxiety adjustment.
Methods: In this experimental study 70 female mice weighing approximately 25 to 30 g were studied in seven treatments groups including control, placebo, anxiety, diazepam, 50, 100, and 200 mg/kg of balm’s hydro-alcoholic extract. Drug and extract doses were injected IP. After receiving the last dose, anxiety was induced using dark box and was evaluated by an elevated plus-maze. In order to increase the activity, the animals were kept in a box with black walls for 5 minutes. Then, in order to evaluate the response of anxiety in elevated plus maze transferred and count the time spent in the open arms for 5 minutes (as an indicator of anxiety) were observed and recorded. Obtained data were analyzed using SPSS 11.5 software.
Results: The results of tests on the treatment showed that the hydro alcoholic extract in 200 mg/kg dose increased the time of presence in open arms significantly which shows anxiety reduction. Also, movement activities of mice were significantly higher in this dose in proportion to diazepam.
Conclusion: According to results, hydro alcoholic extract of balm in 200 mg/kg dose can be a suitable replacement for diazepam in reducing anxiety reflexes. |
| first_indexed | 2024-04-12T12:13:26Z |
| format | Article |
| id | doaj.art-5510666ee0ee4b819a2196450c8b6938 |
| institution | Directory Open Access Journal |
| issn | 1728-6506 1728-6514 |
| language | fas |
| last_indexed | 2024-04-12T12:13:26Z |
| publishDate | 2016-01-01 |
| publisher | Yasuj University Of Medical Sciences |
| record_format | Article |
| series | Armaghane Danesh Bimonthly Journal |
| spelling | doaj.art-5510666ee0ee4b819a2196450c8b69382022-12-22T03:33:31ZfasYasuj University Of Medical SciencesArmaghane Danesh Bimonthly Journal1728-65061728-65142016-01-012010848857Comparative effects of balm hydro alcoholic extract and diazepam on reducing anxiety of in miceM Modaresi0M Basravi1I Sajadia2 Department of Physiology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran 2Department of Psychology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran 2Department of Psychology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran Background & aim: Anxiety is a normal feeling which is experienced in threatening situations and can affect neural system. Wide spread of anxiety forces many people to permanent use of anti-stress drugs especially benzodiazepines. This study was carried out to compare the effects of balm extract and diazepam on anxiety adjustment. Methods: In this experimental study 70 female mice weighing approximately 25 to 30 g were studied in seven treatments groups including control, placebo, anxiety, diazepam, 50, 100, and 200 mg/kg of balm’s hydro-alcoholic extract. Drug and extract doses were injected IP. After receiving the last dose, anxiety was induced using dark box and was evaluated by an elevated plus-maze. In order to increase the activity, the animals were kept in a box with black walls for 5 minutes. Then, in order to evaluate the response of anxiety in elevated plus maze transferred and count the time spent in the open arms for 5 minutes (as an indicator of anxiety) were observed and recorded. Obtained data were analyzed using SPSS 11.5 software. Results: The results of tests on the treatment showed that the hydro alcoholic extract in 200 mg/kg dose increased the time of presence in open arms significantly which shows anxiety reduction. Also, movement activities of mice were significantly higher in this dose in proportion to diazepam. Conclusion: According to results, hydro alcoholic extract of balm in 200 mg/kg dose can be a suitable replacement for diazepam in reducing anxiety reflexes.http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-300-1&slc_lang=en&sid=1Melissa officinalis Anxiety Diazepam Mice |
| spellingShingle | M Modaresi M Basravi I Sajadia Comparative effects of balm hydro alcoholic extract and diazepam on reducing anxiety of in mice Armaghane Danesh Bimonthly Journal Melissa officinalis Anxiety Diazepam Mice |
| title | Comparative effects of balm hydro alcoholic extract and diazepam on reducing anxiety of in mice |
| title_full | Comparative effects of balm hydro alcoholic extract and diazepam on reducing anxiety of in mice |
| title_fullStr | Comparative effects of balm hydro alcoholic extract and diazepam on reducing anxiety of in mice |
| title_full_unstemmed | Comparative effects of balm hydro alcoholic extract and diazepam on reducing anxiety of in mice |
| title_short | Comparative effects of balm hydro alcoholic extract and diazepam on reducing anxiety of in mice |
| title_sort | comparative effects of balm hydro alcoholic extract and diazepam on reducing anxiety of in mice |
| topic | Melissa officinalis Anxiety Diazepam Mice |
| url | http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-300-1&slc_lang=en&sid=1 |
| work_keys_str_mv | AT mmodaresi comparativeeffectsofbalmhydroalcoholicextractanddiazepamonreducinganxietyofinmice AT mbasravi comparativeeffectsofbalmhydroalcoholicextractanddiazepamonreducinganxietyofinmice AT isajadia comparativeeffectsofbalmhydroalcoholicextractanddiazepamonreducinganxietyofinmice |